A5077014370- Atmospheric chemistry and aerosols
- Atmospheric and Environmental Gas Dynamics
- Atmospheric Ozone and Climate
- Atmospheric aerosols and clouds
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Air Quality and Health Impacts
- Analytical Chemistry and Chromatography
- Isotope Analysis in Ecology
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Advanced Chemical Sensor Technologies
- Astro and Planetary Science
- Bee Products Chemical Analysis
- Groundwater and Isotope Geochemistry
- Vehicle emissions and performance
- Nuclear Physics and Applications
- Methane Hydrates and Related Phenomena
- Air Quality Monitoring and Forecasting
- Healthcare and Venom Research
- Advanced Aircraft Design and Technologies
- Enzyme Catalysis and Immobilization
- Protein Structure and Dynamics
- Insect and Pesticide Research
- Energy and Environment Impacts
Max Planck Institute for Chemistry
2017-2022
Johannes Gutenberg University Mainz
2017-2021
Utrecht University
1983-2016
Unilever (Netherlands)
1990-2016
University of Eastern Finland
2013-2016
Vrije Universiteit Amsterdam
2004
Institute of Molecular Life Sciences
1990
Unilever (United Kingdom)
1990
Abstract The European Union (EU)-funded project Dynamics–Aerosol–Chemistry–Cloud Interactions in West Africa (DACCIWA) investigates the relationship between weather, climate, and air pollution southern Africa—an area with rapid population growth, urbanization, an increase anthropogenic aerosol emissions. over this region contains a unique mixture of natural gases, liquid droplets, particles, emitted environment which multilayer clouds frequently form. These exert large influence on local...
The interaction of cardiotoxin II Naja mossambica with cardiolipin model membranes was investigated by binding, fluorescence, resonance energy transfer, fluorescence quenching, 31P NMR, freeze-fracture, and small-angle X-ray experiments. An initially electrostatic binding appeared to be accompanied a deep penetration, most likely into the acyl chain region phospholipids, indicating hydrophobic contribution strong (KD congruent 5 X 10(-8) M). This results in fusion unilamellar vesicles as...
In order to obtain more insight in the initial steps of process protein translocation across membranes, biophysical investigations were undertaken on lipid specificity and structural consequences penetration PhoE signal peptide into model membranes conformation adopted upon interaction with lipids. When monolayer technique differential scanning calorimetry are used, a stronger is observed for negatively charged lipids, significantly influenced by physical state but not temperature or acyl...
Abstract. Vast stretches of agricultural land in southern and central Africa are burnt between June September each year, which releases large quantities aerosol into the atmosphere. The resulting smoke plumes carried west over Atlantic Ocean at altitudes 2 4 km. As only limited observational data West have existed until now, whether this pollution has an impact lower remained unclear. Dynamics-aerosol-chemistry-cloud interactions (DACCIWA) aircraft campaign took place during July 2016, with...
The lipA gene encoding the extracellular lipase produced by Pseudomonas glumae PG1 was cloned and characterized. A sequence analysis revealed an open reading frame of 358 codons mature (319 amino acids) preceded a rather long signal 39 acids. As first step in structure-function analysis, we determined Ser-Asp-His triad which makes up catalytic site this lipase. On basis primary homology with other known lipases, number putative active residues located conserved areas were found. To determine...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMelittin-induced changes of the macroscopic structure phosphatidylethanolaminesA. M. Batenburg, J. H. Van Esch, and B. De KruijffCite this: Biochemistry 1988, 27, 7, 2324–2331Publication Date (Print):April 5, 1988Publication History Published online1 May 2002Published inissue 5 April 1988https://pubs.acs.org/doi/10.1021/bi00407a013https://doi.org/10.1021/bi00407a013research-articleACS PublicationsRequest reuse permissionsArticle...
The behavior of the chemically synthesized PhoE signal peptide and fragments on hydrophilic-hydrophobic interfaces was studied with circular dichroism monolayer techniques. experimental results were compared computer-calculated predictions structure, orientation, molecular area. complete sequence found to aggregate in a beta-sheet structure when introduced an aqueous environment; other hand, sodium dodecyl sulfate micelles approximately 75% alpha-helical observed. Assuming this reflect...
A characterization of the structural alterations induced by melittin in model-membranes dioleoylphosphatidic acid and egg phosphatidylglycerol is presented, based on use 31P-NMR, freeze-fracture electron microscopy small angle X-ray scattering. In accordance with earlier findings cardiolipin-melittin system, found to have an inverted phase inducing effect these negatively charged lipids, contrast influence zwitterionic phospholipids. phosphatidic this expressed formation HII phase; a less...
Abstract. The isotopic composition of molecular hydrogen (H2) contains independent information for constraining the global H2 budget. To explore this, we have implemented sources and sinks, including their stable isotope fractionation constants, into chemistry transport model TM5. For first time, a now includes simplified but explicit reaction scheme photochemical production H2. We present comparison modelled results mixing ratio with available measurements on seasonal to inter annual time...
Abstract. As part of the Dynamics-Aerosol-Chemistry-Cloud Interactions in West Africa (DACCIWA) project, an airborne campaign was designed to measure a large range atmospheric constituents, focusing on effect anthropogenic emissions regional climate. The presented study details results French ATR42 research aircraft, which aimed characterize gas-phase, aerosol and cloud properties region during field carried out June/July 2016 combination with German Falcon 20 British Twin Otter aircraft....
This work reassesses the global atmospheric budget of H 2 with TM5 model. The recent adjustment calibration scale for translates into a change in tropospheric burden. Furthermore, ECMWF Reanalysis‐Interim (ERA‐Interim) data from European Centre Medium‐Range Weather Forecasts (ECMWF) used this study show slower vertical transport than operational before. Consequently, more is removed by deposition. deposition parametrization updated because significant fluxes snow, water, and vegetation...
An obstacle in the application of many "health ingredients" is their alleged off-flavor. We used a combination chemical, sensory, and biological analyses to identify bitter components citrus peel-derived polymethoxyflavone preparations, claimed be functional lowering cholesterol. Nobiletin (56-81%) tangeretin (10-33%) were found main components. Using vitro receptor assays, hTAS2R14 was shown involved perception, with EC50 values 14 63 μM, respectively. Our analysis provided several routes...
The feasibility of stabilizing proteins towards proteolytic degradation was explored by engineering the primary cleavage site(s). This novel approach does not require information on 3-D structure native enzyme. As a model system, extracellular lipase Pseudomonas glumae chosen, which is sensitive subtilisin-tvpe proteases. in appeared to be located between amino acids serine 153 and histidine 154. Since subtUisins are known show preference acid residues surrounding scissile bond,...
Abstract. Despite the potential of isotope measurements to improve our understanding global atmospheric molecular hydrogen (H2) cycle, few H2 data have been published so far. Now, within EUROpean network for HYDRogen Observations and Studies project (EUROHYDROS), weekly monthly air samples from six locations in a sampling analysed mixing ratio (m(H2)) stable isotopic composition (δ(D,H2), hereafter referred as δD). The time series thus obtained now cover one five years all stations. This is...
Abstract. In-situ, quasi-continuous measurements of atmospheric hydrogen (H2) have been performed since October 2007 at the Cabauw tall tower station in Netherlands. Mole fractions H2, CO and several greenhouse gases are determined simultaneously air sampled successively four heights, between 20 200 m above ground level. 222Rn m. This H2 dataset represents first in-situ, long-term measurement series vertical profiles lower continental boundary layer. Seasonal cycles present all heights both...
Abstract. Molecular hydrogen (H2) is the second most abundant reduced trace gas (after methane) in atmosphere, but its biogeochemical cycle not well understood. Our study focuses on soil production and uptake of H2 associated isotope effects. Air samples from a grass field forest site Netherlands were collected using chambers. The results show that emission occurred simultaneously at all sampling sites, with strongest grassland sites where clover (N2 fixing legume) was present. mole fraction...