A5084134590- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Cell death mechanisms and regulation
- DNA Repair Mechanisms
- Viral-associated cancers and disorders
- Systemic Lupus Erythematosus Research
- Glycosylation and Glycoproteins Research
- NF-κB Signaling Pathways
- RNA Research and Splicing
- Lymphoma Diagnosis and Treatment
- Histiocytic Disorders and Treatments
- Inflammatory mediators and NSAID effects
- Renal Transplantation Outcomes and Treatments
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Cancer, Lipids, and Metabolism
- RNA Interference and Gene Delivery
- Platelet Disorders and Treatments
- Endoplasmic Reticulum Stress and Disease
- Galectins and Cancer Biology
- interferon and immune responses
- Protein purification and stability
- Cancer, Hypoxia, and Metabolism
- Autoimmune Bullous Skin Diseases
Chinese University of Hong Kong
2006-2019
Prince of Wales Hospital
2000-2014
Shantou University
2008
Shantou University Medical College
2008
Key Laboratory of Guangdong Province
2008
Southeast University
2008
Medical Research Council
1995
MRC Laboratory of Molecular Biology
1995
Hammersmith Hospital
1985
Epstein-Barr virus (EBV) latent infection is a critical event in nasopharyngeal carcinoma (NPC) tumorigenesis. EBV-encoded genes have been shown to be involved immune evasion and the regulation of various cellular signaling cascades. To elucidate roles EBV NPC development, stable epithelial cell lines was established. Similar primary tumors NPC, these infected cells exhibited type II latency expression pattern. In this study, multiple pathways EBV-infected were investigated. We first...
Epstein-Barr virus (EBV) is associated with several human diseases including infectious mononucleosis and nasopharyngeal carcinoma. EBV-encoded latent membrane protein 1 (LMP1) oncogenic indispensable for cellular transformation caused by EBV. Expression of LMP1 in host cells constitutively activates both the c-Jun N-terminal kinase (JNK) NF-kappaB pathways, which contributes to effect LMP1. However, underlying signaling mechanisms are not very well understood. Based mainly on overexpression...
BRE, brain and reproductive organ-expressed protein, was found previously to bind the intracellular juxtamembrane domain of a ubiquitous death receptor, tumor necrosis factor receptor 1 (TNF-R1), down-regulate TNF-α-induced activation NF-κB. Here we show that BRE also binds another Fas, upon overexpression conferred resistance apoptosis induced by TNF-α, anti-Fas agonist antibody, cycloheximide, variety stress-related stimuli. However, down-regulation endogenous small interfering RNA...
Abstract Co‐deletion of chromosomes 1p and 19q is a common event in oligodendroglial tumors (OTs), suggesting the presence OT‐related genes. The aim this study was to identify target genes residing minimally deleted regions on chromosome 1p36.31–p36.32 that might be involved OTs. A novel gene KIAA0495/p53‐dependent apoptosis modulator (PDAM) found frequently deregulated, with 37 58 (63.8%) OTs examined showing reduced expression compared normal brain. Chromosome loss epigenetic modifications...
Abstract Epstein‐Barr virus (EBV) is closely associated with nasopharyngeal carcinoma (NPC), a common cancer in Hong Kong. The EBV‐encoded LMP1 protein believed to play an important role cell transformation. We have previously identified prevalent variant (2117‐LMP1) that expressed 86% of primary NPC In this study, the biologic phenotypes induced by 2117‐LMP1 were compared those prototypic B95.8‐LMP1 immortalized epithelial line, NP69. could induce proliferation and resistance apoptosis...
We previously demonstrated that the Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) potently activates cellular c-Jun N-terminal kinase (JNK) pathway by sequentially engaging an unknown adaptor, TRAF6, TAB1/TAK1, and JNKKs. now show BS69, a MYND domain-containing protein, is missing adaptor bridges LMP1 as domain separate region of BS69 bind to carboxyl termini respectively. While promotes interaction between complex formation TRAF6 dependent. A fraction constitutively...
<b>Background:</b> It has been proved that cyclo-oxygenase-2 (COX-2) is rapidly induced by inflammatory mediators. However, it not known whether overexpression of COX-2 in the liver sufficient to promote activation or secretion factors leading hepatitis. <b>Aim:</b> To investigate role forced expression using inducible transgenic (TG) mice. <b>Methods:</b> TG mice overexpress human gene liver-specific transthyretin promoter and non-TG littermates were derived fed normal diet for up 12...
Cyclooxoygenase (COX)-2 overexpression is involved in gastric carcinogenesis. While high-salt intake a known risk factor for cancer development, we determined the effects of high salt on chemical carcinogenesis COX-2 transgenic (TG) mice. TG mice were developed C57/BL6 strain using full-length human cox-2 complementary DNA construct. Six-week-old and wild-type (WT) littermates randomly allocated to receive alternate week N -methyl- -nitrosourea (MNU, 240 p.p.m.) drinking water or control 10...
Cytokines are important mediators involved in the development of effector cells and regulation immune responses. The gene expression these T cell subset has yet to be fully elucidated. Using sensitive reverse transcription-polymerase chain reaction (RT-PCR), kinetics cytokine human CD4+ CD8+ were examined. more readily activated by phorbol myristate acetate (PMA) phytohaemagglutinin (PHA) than terms IL-2 receptor (IL-2R) mRNA expression. Quantitative differences between confirmed higher...
Esophageal tumorigenesis is a complex and cascading process, involving the interaction of many genes proteins. In this study, we have used comparative proteomic approach to identify tumor-associated proteins explore carcinogenic mechanisms. Two-dimensional electrophoresis (2-DE) MALDI-TOF MS analysis esophageal carcinoma control cells revealed 10 that were upregulated. A further downregulated. Among these 20 differentially expressed proteins, brain reproductive organ-expressed (BRE) protein...
Abstract IgA nephropathy (IgAN) is characterized by raised serum 1 deposits. We have previously shown increased T‐cell activation in IgAN. Recently, transforming growth factor‐β (TGF‐β) has been to induce isotype switch at a clonal level and interleukin 5 (IL5) promotes differentiation into IgA‐bearing B cells. In the present study we examined TGF‐β IL5 mRNA expression mitogen‐activated CD4‐positive T cells from patients with IgAN ( n =25), other primary nephritides (CGN) =24), healthy...
The brain and reproductive organ expressed (BRE) gene encodes a highly conserved stress-modulating protein. To gain further insight into the function of this gene, we used comparative proteomics to investigate protein profiles C2C12 D122 cells resulting from small interfering RNA (siRNA)-mediated silencing as well overexpression BRE. Silencing BRE in cells, using siRNA, resulted up-regulated Akt-3 carbonic anhydrase III expression, while 26S proteasome regulatory subunit S14 prohibitin were...
BRE is a multifunctional adapter protein involved in DNA repair, cell survival and stress response. To date, most studies of this have been focused the tumor model. The role stem biology has never investigated. Therefore, we used HUCPV progenitor cells to elucidate function BRE. are multipotent fetal which possess ability differentiate into multitude mesenchymal lineages when chemically induced can be more easily amplified culture. In study, established that expression was normally expressed...
Abstract The BRE gene, alias BRCC45, produces a 44 kDa protein that is normally distributed in both cytoplasm and nucleus. In this study, we used adult fibroblasts isolated from wild-type (WT) knockout (BRE −/− ) mice to investigate the functional role of DNA repair cellular senescence. We compared WT with at different cell passages observed mutant entered replicative senescence earlier than fibroblasts. With use gamma irradiation induce damage fibroblasts, percentage SA-β-Gal + cells was...
The origin of anti-DNA antibodies remains speculative. We argue that some these may arise inadvertently in nature during the course a normal immune response due to their induction by which bear structures (mimotopes) mimic DNA. These are not necessarily DNA specific but, like T15 idiotype (id)-positive bind phosphorylcholine, produced normally environmental or microbial antigen. Such mimotope was found T15+ antibody at highly region encoded principally D gene, DFL16.1. This also human DXP'1,...
Abstract In this study, we used comparative proteomics to identify proteins that were involved in the regulation of interdigital cell death. The protein profiles embryonic day (E) 12.5 and 13.5 mouse hindlimb tissues compared differentially expressed. cells are irreversibly committed programmed death (PCD) at E13.5, whereas they developmentally plastic E12.5. We established disulfide isomerase (PDI) expression was up‐regulated while peroxiredoxin 1 (Prdx1) down‐regulated time point....