A5050182392- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- RNA regulation and disease
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
Friedrich Miescher Institute
2017-2022
University of Basel
2021-2022
University of Tübingen
2015
Site-directed RNA editing allows for the manipulation of and protein function by reprogramming genetic information at level. For this we assemble artificial RNA-guided editases demonstrate their transcript repair activity in cells developing embryos annelid Platynereis dumerilii. A hallmark our assembly strategy is covalent attachment guideRNA enzyme applying SNAP-tag technology, a process that here to be readily triggered light vitro, mammalian cell culture, also P. Lacking both...
Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed have been intensely studied, regulatory circuits protect active from inactivating signals are not well understood. Here we report a positive feedback loop preserves the transcription-competent state RNA polymerase II-transcribed genes. We found Pdp3 recruits histone acetyltransferase Mst2...
Methylation of histone H3 at lysine 9 (H3K9) is a hallmark heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- tri-methylation H3K9. Although H3K9 methylation has been intensely studied mitotic cells, its role during sexual differentiation remains unclear. Here, we map genome-wide meiosis show constitutive temporarily loses H3K9me2 becomes H3K9me3 when cells commit to meiosis. Cells...
Small non-protein coding RNAs are involved in pathways that control the genome at level of chromatin. In Schizosaccharomyces pombe, small interfering (siRNAs) required for faithful propagation heterochromatin is found peri-centromeric repeats. contrast to repetitive DNA, protein-coding genes refractory siRNA-mediated formation, unless siRNAs expressed mutant cells. Here we report identification 20 novel alleles enable de novo formation a euchromatic gene by using trans-acting as triggers....