A5075136946- Gene Regulatory Network Analysis
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
- Cancer-related gene regulation
- Advanced Drug Delivery Systems
- Fungal and yeast genetics research
- COVID-19 Clinical Research Studies
- Mathematical Biology Tumor Growth
- COVID-19 epidemiological studies
- Microbial Metabolic Engineering and Bioproduction
- Mosquito-borne diseases and control
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Oral and gingival health research
- Malaria Research and Control
- Antibiotics Pharmacokinetics and Efficacy
- Chemical Reactions and Isotopes
- RNA modifications and cancer
- Pharmacological Effects and Toxicity Studies
- Mathematical and Theoretical Epidemiology and Ecology Models
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
Joint Research Centre
2024
European Commission
2024
University of Veterinary Medicine Hannover, Foundation
2023
University of Veterinary Medicine
2023
Technical University of Munich
2019-2022
Helmholtz Zentrum München
2019-2022
University of Pennsylvania
2019-2020
Chromatin states must be maintained during cell proliferation to uphold cellular identity and genome integrity. Inheritance of histone modifications is central in this process. However, the modification landscape challenged by incorporation new unmodified histones each cycle, principles governing heritability remain unclear. We take a quantitative computational modeling approach describe propagation H3K27 H3K36 methylation states. measure combinatorial patterns mass spectrometry on...
Interactions between SARS-CoV-2 and the immune system during infection are complex. However, understanding within-host dynamics is of enormous importance for clinical public health outcomes. Current mathematical models focus on describing acute phase. Thereby they ignore important long-term post-acute effects. We present a model, which not only describes phase, but extends current approaches by also recapitulating clinically observed effects, such as recovery number susceptible epithelial...
The effectiveness of antivirals in mitigating influenza outbreaks depends on both their ability to reduce the number infections and risk drug resistance. We extended a previously developed mathematical model investigate impact mitigation strategies, including mono or combination antiviral treatment chemoprophylaxis vaccination, transmission dynamics. Our findings indicate that is more effective than reducing burden, except when resistant strain has high rate, which case may trigger...
ABSTRACT The effectiveness of antiviral treatment with remdesivir against COVID-19 has been investigated in clinical trials suggesting earlier recovery. However, this effect seems to be rather modest. In study, we assessed the course SARS-CoV-2 infections 369 individuals across a spectrum illness severities, including both untreated and who received remdesivir. Moreover, using process-based mathematical model, quantified analyzed within-host infection dynamics 69 19 antiviral-treated...
DNA replication during cell division leads to dilution of histone modifications and can thus affect chromatin-mediated gene regulation, raising the question how cell-cycle shapes modification landscape, particularly embryogenesis. We tackled this problem by manipulating cycle early Xenopus laevis embryogenesis analyzing in vivo H4K20 methylation kinetics. The global distribution un-, mono-, di-, tri-methylated was measured mass spectrometry normal cell-cycle-arrested embryos over time. Using...
Cells must continuously adjust to changing environments and, thus, have evolved mechanisms allowing them respond repeated stimuli. While faster gene induction upon a stimulus is known as reinduction memory, responses repression been less studied so far. Here, we across carbon source shifts in over 1,500 single Saccharomyces cerevisiae cells. By monitoring the expression of source-responsive gene, galactokinase 1 ( Gal1 ), and fitting mathematical model single-cell data, observed response...
The effectiveness of antiviral treatment with remdesivir against COVID-19 has been investigated in clinical trials suggesting earlier recovery. However, this effect seems to be rather modest. In study, we tracked the course SARS-CoV-2 infections 369 individuals across a spectrum illness severities, including both untreated and who received remdesivir. Moreover, using process-based mathematical model, quantified analysed within-host infection dynamics total 88 individuals, which 69 were 19...
SUMMARY Chromatin states must be maintained during cell proliferation to uphold cellular identity and genome integrity. Inheritance of histone modifications is central in this process. However, the modification landscape challenged by incorporation new unmodified histones each cycle principles governing heritability remain unclear. Here, we take a quantitative computational modeling approach describes propagation K27 K36 methylation states. We measure combinatorial patterns mass spectrometry...
SUMMARY Histone modifications regulate chromatin architecture and thereby control gene expression. Rapid cell divisions DNA replication however lead to a dilution of histone can thus affect mediated regulation So how does the cell-cycle shape modification landscape, in particular during embryogenesis when fast precise cell-specific expression is required? We addressed this question vivo by manipulating early Xenopus laevis embryogenesis. The global distribution un-, mono- di- tri-methylated...
This study aimed to determine a pharmacokinetic profile for single dosage of cyclosporine A (CsA) clinically used immunosuppression in cats. Blood-CsA-concentrations were measured before and 1, 2, 4, 6, 8, 12 24 hours after oral administration 7 mg/kg body weight (BW) CsA (Atopica® solution) 8 healthy adult cats using high-performance liquid chromatography coupled mass spectrometry. Pharmacokinetic parameters calculated WinNonLin software mainly based on 1-compartment-model additional...
Interactions between SARS-CoV-2 and the immune system during infection are complex. However, understanding within-host dynamics is of enormous importance, especially when it comes to assessing treatment options. Mathematical models have been developed describe dissect mechanisms underlying COVID-19 pathogenesis. Current mathematical focus on acute phase, thereby ignoring important post-acute effects. We present a model, which not only describes but also reflects recovery number susceptible...
SUMMARY Non-genetic transcriptional variability at the single-cell level is a potential mechanism for therapy resistance in melanoma. Specifically, rare subpopulations of melanoma cells occupy transient pre-resistant state characterized by coordinated high expression several genes. Importantly, these are able to survive drug treatment and develop resistance. How might extremely states arise disappear within population? It unclear whether canonical stochastic models probabilistic pulsing can...
ABSTRACT Cells must continuously adapt to changing environments and, thus, have evolved mechanisms allowing them respond repeated stimuli. For example, faster gene induction upon a stimulus aids adaptation - process known as reinduction memory. However, whether such memory exists for repression is unclear. Here, we studied across carbon source shifts in over 2,500 single Saccharomyces cerevisiae cells. By monitoring the expression of source-responsive gene, galactokinase 1 ( Gal1 ), and...
ABSTRACT Accurately functioning genetic networks should be responsive to signals but prevent transmission of stochastic bursts expression. Existing data in mammalian cells suggests that such transcriptional “noise” is transmitted by some genes and not others, suggesting noise tunable, perhaps at the expense other signal processing capabilities.However, systematic claims about have been limited inability directly measure transmission. Here we build a mathematical framework capable modeling...