A5048051646- RNA Research and Splicing
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Wnt/β-catenin signaling in development and cancer
- Advanced Fluorescence Microscopy Techniques
- Mathematical Biology Tumor Growth
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Heat shock proteins research
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Hippo pathway signaling and YAP/TAZ
- Nanopore and Nanochannel Transport Studies
- Arctic and Antarctic ice dynamics
- Probabilistic and Robust Engineering Design
- Art Therapy and Mental Health
- Neuroscience and Neural Engineering
- Advanced Mathematical Modeling in Engineering
- Digestive system and related health
- Educational Methods and Teacher Development
- Microtubule and mitosis dynamics
- Tissue Engineering and Regenerative Medicine
- stochastic dynamics and bifurcation
- Neural dynamics and brain function
University of Notre Dame
2023-2024
University of Ottawa
2021-2022
University of North Carolina at Chapel Hill
2019-2021
Florida State University
2020
North Carolina State University
2017-2018
The RNA binding protein TDP-43 forms intranuclear or cytoplasmic aggregates in age-related neurodegenerative diseases. In this study, we found that binding-deficient (produced by neurodegeneration-causing mutations posttranslational acetylation its recognition motifs) drove demixing into liquid spherical shells with cores. These droplets, which named "anisosomes", have exhibit birefringence, thus indicating crystal formation. Guided mathematical modeling, identified the primary components of...
An emerging mechanism for intracellular organization is liquid-liquid phase separation (LLPS). Found in both the nucleus and cytoplasm, liquidlike droplets condense to create compartments that are thought promote inhibit specific biochemistry. In this work, a multiphase, Cahn-Hilliard diffuse interface model used examine RNA-protein interactions driving LLPS. We bivalent system allows two different species of protein-RNA complexes competition arises shared binding partner, free protein. With...
Following the formation of a primary carcinoma, neoplastic cells metastasize by undergoing epithelial mesenchymal transition (EMT), which is triggered cues from inflammatory and stromal in microenvironment. EMT allows to lose their highly adhesive nature instead adopt spindle-like appearance, as well invasive migratory behavior, cells. We hypothesize that bistable switch between phenotypes governs EMT, allowing cell maintain its phenotype even after it leaves tumor microenvironment...
Abstract Liquid-liquid phase separation is an intracellular mechanism by which molecules, usually proteins and RNAs, interact then rapidly demix from the surrounding matrix to form membrane-less compartments necessary for cellular function. Occurring in both cytoplasm nucleus, properties of resulting droplets depend on a variety characteristics specific molecules involved, such as valency, density, diffusion within crowded environment. Capturing these complexities biologically relevant model...
The epithelial mesenchymal transition (EMT) is one step in the process through which carcinoma cells metastasize by gaining cellular mobility associated with cells. This work examines dual influence of TGF-β pathway and intercellular contact on activation EMT colon (SW480) breast (MCF7) While SW480 population revealed an intermediate state between states, MC7 exhibited highly adhesive behavior. However, for both cell lines, exogenous signal a reduction confluence can push subgroup towards...
Abstract The RNA binding protein TDP-43 naturally phase separates within cell nuclei and forms cytoplasmic aggregates in age-related neurodegenerative diseases. Here we show that acetylation-mediated inhibition of to produces co-de-mixing acetylated unmodified into symmetrical, intranuclear spherical annuli whose shells cores have liquid properties. Shells are anisotropic, like crystals. Consistent with our modelling predictions annulus formation is driven by components strong...
Abstract Partial Rank Correlation Coefficient (PRCC) is a powerful type of global sensitivity analysis. Usually performed following Latin Hypercube Sampling (LHS), this analysis can highlight the parameters in mathematical model producing observed results, crucial step when using models to understand real-world phenomena and guide future experiments. Recently, Gasior et al. LHS PRCC modeling influence cell–cell contact TGF- $$\beta $$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML">...
Abstract Actin is an intracellular protein that constitutes a primary component of the cellular cytoskeleton and accordingly crucial for various cell functions. assembles into semi-flexible filaments cross-link to form higher order structures within cytoskeleton. In turn, actin cytoskeketon regulates shape, participates in migration division. A variety theoretical models have been proposed investigate dynamics across distinct scales, from stochastic nature molecular motor deterministic...
Cells rely on their cytoskeleton for key processes including division and directed motility. Actin filaments are a primary constituent of the cytoskeleton. Although actin can create variety network architectures linked to distinct cell functions, microscale molecular interactions that give rise these macroscale structures not well understood. In this work, we investigate mechanisms produce different branched using an iterative classification approach. First, employ simple yet comprehensive...
Abstract Cells rely on their cytoskeleton for key processes including division and directed motility. Actin filaments are a primary constituent of the cytoskeleton. Although actin can create variety network architectures linked to distinct cell functions, microscale molecular interactions that give rise these macroscale structures not well understood. In this work, we investigate mechanisms produce different branched using an iterative classification approach. First, employ simple yet...