Increasing studies have shown that obesity is the primary cause of cardiovascular diseases, non-alcoholic fatty liver type 2 diabetes, and a variety cancers. The dysfunction gut microbiota was proved to result in obesity. Recent research indicated ANGPTL4 key regulator lipid metabolism circulating medium for fat deposition. present study conducted investigate alteration expression gastrointestinal tract mice treated by high-fat diet. Ten C57BL/6J were randomly allocated two groups fed with...

Sucralose is a non-nutritive artificial sweetener (NNS) used in foods or beverages to control blood glucose levels and body weight gain. The consumption of NNS has increased recent years over the world, many researches have indicated long-term sucralose administration altered gut microbiome composition mice. These studies all focus on US Food Drug Administration (FDA) defined acceptable daily intake (ADI), approximately 5 mg/kg BW/day for human. In our study, mice were given with T1-4...

Excessive utilization of chemical fertilizers degrades the quality medicinal plants and soil. Bio-organic (BOFs) including microbial inoculants microalgae have garnered considerable attention as potential substitutes for fertilizer to enhance yield. In this study, a field experiment was conducted investigate effects BOF partially substituting on growth plant Polygala tenuifolia. The parameters, bioactive component contents, soil properties composition rhizosphere microorganisms were...

Abstract Quorum sensing (QS) is a process that enables bacteria to communicate using secreted signaling molecules, and then makes population of regulate gene expression collectively control behavior on community-wide scale. Theoretical studies efficiency have suggested both mass-transfer performance in the local environment spatial distribution cells are key factors affecting QS. Here, an experimental model based hydrogel microcapsules with three-dimensional structure was established...

Rationale: PCDH19-related epilepsy manifested various clinical features, including febrile epilepsy, with or without intellectual disability, and psych-behavioral disorders. However, there are few studies demonstrating abdominal pain as the first symptom. Patient concerns: A 3-year-old Chinese girl presented clustered seizures of fever sensitivity accompanied by pain. Diagnoses: After ultrasonography ruled out organic lesions, electroencephalographic (EEG) identified was a seizure feature....

Late gestation is a critical period for regulating maternal peripartum physiological metabolism and gut microbiota balance. Fermented diets have been widely recognized as effective exogenous nutritional interventions capable of modulating the maintenance homeostasis. However, mechanism through which fermented modulate in late-gestation remains poorly understood. In this study, an vitro fermentation model combined with chemical composition analysis, untargeted metabolomics, high-throughput...

The present study was conducted to investigate the effects of dietary supplementation selenium from different sources on growth performance, nutrient digestibility, and blood immune indices piglets orally challenged with Salmonella typhimurium (ST). In a 2 × factorial arrangement, 32 (6.43 ± 0.54 kg body mass) were assigned into four groups or without inclusion sodium selenite (SS) yeast (YS) ST challenge (5 ml 1 10 9 cfu/ml 5 saline) d 13. each period, YS increased average daily feed intake...

Owing to its unique physiochemical properties similar the extracellular matrix (ECM), three-dimensional (3D) crosslinked hydrogels are widely studied materials for tissue engineering. In this study, mimic ECM microenvironment, a two-step covalent cross-linking with hyaluronic acid and gelatin was performed form an interpenetrating polymer network structure. Gelatin as first greatly improved mechanical strength of hydrogels, while second tenacity biological activity. Compared single hydrogel,...

The objective of this study was to investigate the rumen degradation characteristics grain amaranth hay (Amaranthus hypochondriacus) at four different growth stages. aim evaluate nutritional value stages by chemical composition, in vivo digestibility, and situ degradability. Three Boer goats with permanent ruminal fistulas were selected study. Amaranthus (squaring stage (SS), initial bloom (IS), full-bloom (FS) mature (MS)) crushed placed into nylon bags. Each sample set up three replicates,...

Regulation of the expression prepro-TRH (ppTRH) gene by epidermal growth factor (EGF) was investigated. The i.p. injection EGF significantly stimulated hypothalamic ppTRH messenger RNA levels in rats. To clarify whether this stimulatory effect could be exerted at level transcription, 5'-flanking region (-1893/+127) mouse fused to a luciferase reporter transiently transfected into pituitary GH4C1 cells, and on transcription measured assay. promoter activity time- dose-dependent manner....

The study was conducted to investigate the effects of maternal supplementation with rare earth elements (REEs) on sows and their offspring. During late gestation, 120 multiparous were divided randomly into control group (Basal diet) REE-G diet supplemented 200 mg REE/kg). After delivery, further two groups: REE-L- (Change basal during lactation) REE-L+ (REE all time). Our results showed that REE improved antioxidant immunity piglets. Additionally, supply gestation significantly decreased...

The present study was carried out in Luoma Lake, a shallow lake temperate eastern China. Based on two-year study, the dynamics of phytoplankton functional groups and influencing factors were analyzed. A total 178 taxa identified sorted into 20 codons, according to group classification. In order find environmental driving variations, fifteen analyzed detail using redundancy analysis. Groups P (Fragilaria crotonensis), X2 (Chlamydomonas globosa, C. microsphaera Chroomonas acuta), MP (Navicula...

Nerium oleander is one of the most poisonous plants, and its accidental ingestion has frequently occurred in humans livestock. It vital to develop a rapid accurate identification method for timely rescue oleander-poisoned patients investigation poisoning cases. In this study, specific highly sensitive quantitative real-time PCR (qPCR)-based was developed identify mixture systems simulated forensic specimens (SFS). First, new pair oleander-specific primers, JZT-BF/BR, designed validated....

Unsaturated fatty acids with odd-numbered double bonds, e.g. oleic acid, can be degraded by β-oxidation via the isomerase-dependent pathway or reductase-dependent that differ respect to metabolism of bond. In an attempt elucidate metabolic functions two pathways and determine their contributions unsaturated acids, degradation 2-trans,5-cis-tetradecadienoyl-CoA, a metabolite was studied rat heart mitochondria. Kinetic measurements cofactor formation demonstrated more than 80% oleate occurs...

Chinese patent medicines (CPMs) are of great value for the prevention and treatment diseases. However, adulterants pesticide residues in CPMs have become "bottleneck" impeding globalization traditional medicine. In this study, 12 batches commercially available Qipi pill (a famous CPM recorded Pharmacopeia) from different manufacturers were investigated to evaluate their authenticity quality safety. Considering severely degraded DNA CPMs, kompetitive allele specific PCR (KASP) technology...