A5100723655- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Advanced NMR Techniques and Applications
- Lipid Membrane Structure and Behavior
- Antimicrobial Resistance in Staphylococcus
- Bacterial Infections and Vaccines
- Quantum-Dot Cellular Automata
- Carcinogens and Genotoxicity Assessment
- Analytical Methods in Pharmaceuticals
- Inflammatory mediators and NSAID effects
- Chromosomal and Genetic Variations
- Knee injuries and reconstruction techniques
- Nitric Oxide and Endothelin Effects
- Microbial Metabolism and Applications
- RNA Interference and Gene Delivery
- Dye analysis and toxicity
- Antibiotic Resistance in Bacteria
- Neuroinflammation and Neurodegeneration Mechanisms
- Analytical Chemistry and Chromatography
- Glycogen Storage Diseases and Myoclonus
- Lipid metabolism and biosynthesis
- Pneumonia and Respiratory Infections
Utrecht University
2021-2025
Aeronautical Development Agency
2022
Royal Preston Hospital
2009
Manchester Royal Infirmary
2009
The University of Texas Health Science Center at San Antonio
1987-1988
Abstract Antibiotics that use novel mechanisms are needed to combat antimicrobial resistance 1–3 . Teixobactin 4 represents a new class of antibiotics with unique chemical scaffold and lack detectable resistance. targets lipid II, precursor peptidoglycan 5 Here we unravel the mechanism teixobactin at atomic level using combination solid-state NMR, microscopy, in vivo assays molecular dynamics simulations. The enduracididine C-terminal headgroup specifically binds pyrophosphate-sugar moiety...
Antimicrobial resistance is a leading mortality factor worldwide. Here, we report the discovery of clovibactin, an antibiotic isolated from uncultured soil bacteria. Clovibactin efficiently kills drug-resistant Gram-positive bacterial pathogens without detectable resistance. Using biochemical assays, solid-state nuclear magnetic resonance, and atomic force microscopy, dissect its mode action. blocks cell wall synthesis by targeting pyrophosphate multiple essential peptidoglycan precursors...
Human cathelicidin LL‐37 is a widely studied antimicrobial 37‐mer peptide with various ascribed functions, that serves as template for designing novel peptides. Its derivative, the 24‐mer SAAP‐148, highly effective in vitro eradicating multidrug‐resistant bacteria, persistent cells, and biofilms, without inducing resistance. SAAP‐148 characterized by high cationic charge (+11) 46% hydrophobicity, which, once folds into an alpha helix, forms wide hydrophobic face. This amphipathic nature...
This paper aims to design logic gates and flip flops using QCA. demonstrates the implementation of gates. (AND GATE, OR NOR NOT EXORGATE, X-NOR NAND GATE) (SR flop, JK D flop T flop) QCA designer tool software. Using QCA, we can reduce power dissipation, increase speed operation, decrease area size. is known as Quantum Dot Cellular Automata, it a transistor less model. We don't need transistors, resistors& capacitors build or any flops. square shape nano-structure that perform computations....
Bacterial non-ribosomally produced peptides (NRPs) form a rich source of antibiotics, including more than 20 these antibiotics that are used in the clinic, such as penicillin G, colistin, vancomycin, and chloramphenicol. Here we report identification, purification, characterization novel NRP, i.e., brevibacillin 2V (lipo-tridecapeptide), from Brevibacillus laterosporus DSM 25. Brevibacillin has strong antimicrobial activity against Gram-positive bacterial pathogens (minimum inhibitory...
The effectiveness of acetolysis as a tool in structural characterization mono-and di-0-alkyl phosphoglycerides was investigated.Surprisingly, it found that the were resistant to attack during acetolysis, whereas mono-ether types, with free hydroxyl function or an ester on carbon-2, easily attacked at glycerolphosphate bond.On other hand, Vitride reduction occurred readily di-ether phosphoglycerides.The implications these findings they relate identification ether phospholipids tissues are discussed.-
Abstract Specific interactions with phospholipids are often critical for the function of proteins or drugs, but studying these at high resolution remains difficult, especially in complex membranes that mimic biological conditions. In principle, molecular could be directly probed by solid‐state NMR (ssNMR). However, due to challenge detect specific lipids mixed liposomes and limited spectral sensitivity, ssNMR studies scarce. Here, using purified 13 C, 15 N‐labeled phospholipids, we show can...
Lipo-tridecapeptides, a class of bacterial non-ribosomally produced peptides, show strong antimicrobial activity against Gram-positive pathogens, including antibiotic-resistant Staphylococcus aureus and Enterococcus spp. However, many these lipo-tridecapeptides have shown high hemolytic cytotoxicity, which has limited their potential to be developed into antibiotics. Recently, we reported novel lipo-tridecapeptide, brevibacillin 2V, showed no human red blood cells at concentration 128 mg/L,...
Bacterial infections present a major global health threat, often displaying resistance to various antibiotics. Lipoteichoic acid (LTA) is vital component of bacterial cell envelopes Gram-positive bacteria, crucial for integrity, division, and host inflammation. Due its essential role LTA biosynthesis are also attractive drug targets, however, there only scant molecular knowledge on precursor molecules in membranes. Here, we report the isolation characterization diglucosyldiacylglycerol (Glc
Abstract Bacterial non-ribosomally produced peptides (NRPs) form a rich source of antibiotics, including more than twenty these antibiotics that are used in the clinic, such as penicillin G, colistin, vancomycin, and chloramphenicol. Here we report identification, purification, characterization novel NRP, i.e., brevibacillin 2V, from Brevibacillus laterosporus DSM 25. Brevibacillin 2V has strong antimicrobial activity against Gram-positive bacterial pathogens, difficult-to-treat...