- Algal biology and biofuel production
- Meat and Animal Product Quality
- Ubiquitin and proteasome pathways
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Plant Virus Research Studies
- Protein Hydrolysis and Bioactive Peptides
- Protein Structure and Dynamics
- 14-3-3 protein interactions
- RNA modifications and cancer
- Legume Nitrogen Fixing Symbiosis
- Calcium signaling and nucleotide metabolism
- Marine and coastal ecosystems
- Microencapsulation and Drying Processes
- Soybean genetics and cultivation
- Lipid metabolism and biosynthesis
- Catalysis for Biomass Conversion
- Microbial Community Ecology and Physiology
- Protein Degradation and Inhibitors
- Phytase and its Applications
- Covalent Organic Framework Applications
- Proteins in Food Systems
- Microbial bioremediation and biosurfactants
Qingdao University
2024
Qingdao Municipal Hospital
2024
Tianjin University of Technology
2023
Dalian University
2021-2023
Dalian University of Technology
2021-2023
Shanghai Institute of Applied Physics
2016-2022
Chinese Academy of Sciences
2011-2022
Shanghai Advanced Research Institute
2022
Shanghai Synchrotron Radiation Facility
2022
Nanjing Agricultural University
2019-2022
Implementation of a non-natural cofactor alternative to the ubiquitous redox nicotinamide adenosine dinucleotide (NAD) is great scientific and biotechnological interest. Several enzymes have been engineered favor cytosine (NCD), smaller-sized NAD analogue. However, molecular interactions involving analogues remain elusive, preventing us from devising more accept those analogues. Here we took semirational approach evolve phosphite dehydrogenase (Pdh) identified variants with substantially...
Acyl-ACP thioesterase (TE) catalyzes the hydrolysis of thioester bonds during type II fatty acid synthesis and directly determines chain length. Most TEs are responsible for recognition 16:0 18:1 substrates, while specific interrupt acyl-ACP elongation at C8–C14. However, acyl selection mechanism TE has not been thoroughly elucidated to date. In this study, crystal structure C12-specific FatB from Umbellularia californica, which consists two independent hotdog domains, was determined. An...
Microalgae are potential starch producers as alternatives to agricultural crops. This study disclosed the effects and mechanism of phosphorus availability exerted on storage production in a starch-producing microalga Tetraselmis subcordiformis nitrogen starvation conditions. Excessive supply facilitated production, which differed from conventional cognition that would inhibit transitory biosynthesis plants. Phosphorus enhanced energy utilization efficiency for biomass production. ADP-glucose...
Aragonite is a metastable mineral, which easily transformed into calcite, and generally difficult to preserve in the stratum. However, large amounts of aragonites were found Paleogene shale Jiyang Depression. The characteristics preservation mechanisms these analyzed through series analytical methods, including cathodoluminescence, field-emission scanning electron microscopy (FESEM), laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), micro-area carbon oxygen isotopes,...
Adiponitrile (ADN) is a critical raw material for nylon 66, and its efficient, green production vital securing the supply chain of key materials. Among domestic ADN methods, traditional butadiene hydrocyanation process faces challenges such as complex procedures, high energy consumption, significant safety risks. In contrast, adipic acid (AA) ammoniation method has garnered attention due to simplified process, product yield, relatively low investment. However, industrial applications still...
The selective production of C4 bulk chemicals from biomass is significant to replace the traditional method petroleum resource. In this work, succinic anhydride (SAN) directly prepared bio-based furanic platform compounds utilizing visible light-induced oxygenation process, in which m-tetraphenyl porphyrin (H2TPP) and molecular oxygen was employed as photocatalyst terminal oxidant, respectively. Under optimal conditions, a 99.9% conversion with 97.8% selectivity SAN obtained furoic acid...
ABSTRACT Soybean mosaic virus (SMV) is one of the most devastating viral pathogens soybean ( Glycine max (L.) Merr). In total, 22 Chinese SMV strains (SC1–SC22) have been classified based on responses 10 cultivars to these pathogens. However, although several SMV‐resistance loci in identified, no gene conferring resistance resistant cultivar (cv.) Kefeng No.1 has cloned and verified. Here, using F 2 ‐derived 3 (F 2:3 ) recombinant inbred line (RIL) populations from a cross between...
Microbial lipids with fatty acid compositional profiles similar to vegetable oils are of great potential for the production biofuels and oleochemicals. However, costs microbial remain prohibitively high broader applications. Microalgae known as third-generation biomass resources may be explored reduce feedstock lipid production. Here, hydrolysates microalga Arthrospira platensis were converted into by oleaginous yeast Cryptococcus curvatus ATCC 20509. Results indicated that hydrothermal...
Microbial fatty acids are synthesized by Type II acid synthase and could be tailored acyl-ACP thioesterase. With the prospects of medium-chain fatty-acid-derivative biofuels, selectivity thioesterase has been studied to control product chain length. Here, we report an alternative approach manipulating acyl carrier protein portion switch length propensity It was demonstrated that ChFatB2 from Cuphea hookeriana preferred C10-ACP C8-ACP with ACP E. coli, while converting preference lanceolate....
Accumulation of β-carotene in Dunaliella salina is highly dependent on light exposure intensity and duration, but quantitative analysis photon numbers received per cell for triggering accumulation not available so far. In this study, experiment results showed that significant occurred after at least 8 h illumination 400 µmol photons·m
The leaves of soybean cultivar ZheA8901 show various symptoms (necrosis, mosaic, and symptomless) when infected with different strains mosaic virus (SMV). Based on a proteomic analysis performed tandem mass tags (TMT), 736 proteins were differentially expressed from samples that showed asymptomatic, necrosis induced by SMV SC3, SC7, SC15, respectively. Among these, GmGSTU13 ascorbate peroxidase (APX) only upregulated in symptomless leaves, protein level determined western blot was consistent...
To study the influence of a helical wire coil on convective heat transfer capacity cylindrical cooling tube, then to obtain enhanced characteristics, comparative analysis is performed between cases with and without based finite element analysis. In particular, specific model tube both smooth inner wall areas area established. By comparing flow velocities, fluid pressures, coefficients, etc., significant effect improving explained quantitatively. addition, mechanism can be described in three...