A5009587954- Vagus Nerve Stimulation Research
- Neuroscience and Neural Engineering
- Cardiac Arrhythmias and Treatments
- Muscle activation and electromyography studies
- Neurological disorders and treatments
- Heart Rate Variability and Autonomic Control
- Peptidase Inhibition and Analysis
- Pain Management and Treatment
- Connective tissue disorders research
- Genetics and Neurodevelopmental Disorders
- Ultrasound and Hyperthermia Applications
- Cardiac pacing and defibrillation studies
- Botulinum Toxin and Related Neurological Disorders
- Autism Spectrum Disorder Research
- EEG and Brain-Computer Interfaces
- Peripheral Nerve Disorders
- Nicotinic Acetylcholine Receptors Study
- Biological Stains and Phytochemicals
- Cancer and Skin Lesions
- Skin and Cellular Biology Research
- Electrical and Bioimpedance Tomography
- Spinal Dysraphism and Malformations
- Molecular Biology Techniques and Applications
- Thermal Regulation in Medicine
- Neonatal skin health care
University College London
2019-2024
University of London
2022
Universidad de Londres
2022
Stellenbosch University
2018-2019
Medical University of South Carolina
1974-1975
Due to the lack of understanding fascicular organisation, vagus nerve stimulation (VNS) leads unwanted off-target effects. Micro-computed tomography (microCT) can be used trace fascicles from periphery and image anatomy. In this study, we present a simple reproducible method for imaging in peripheral nerves with iodine staining microCT determination anatomy organisation. At determined optimal pre-processing steps scanning parameters, protocol allowed segmentation tracking within nerves. This...
Introduction Despite detailed characterization of fascicular organization somatic nerves, the functional anatomy fascicles evident in human and large mammal cervical vagus nerve is unknown. The a prime target for intervention field electroceuticals due to its extensive distribution heart, larynx, lungs, abdominal viscera. However, current practice approved stimulation (VNS) technique stimulate entire nerve. This produces indiscriminate non-targeted effectors undesired side effects. Selective...
Cardiac disease progression reflects the dynamic interaction between adversely remodeled neurohumoral control systems and an abnormal cardiac substrate. Vagal nerve stimulation (VNS) is attractive neuromodulatory option to dampen this interaction; however, it limited by off-target effects. Spatially-selective VNS (sVNS) offers a promising solution induce cardioprotection while mitigating effects specifically targeting pre-ganglionic parasympathetic efferent fibers. This approach also has...
Abstract Imaging compound action potentials (CAPs) in peripheral nerves could help avoid side effects neuromodulation by selective stimulation of identified fascicles. Existing methods have low resolution, limited imaging depth, or are invasive. Fast neural electrical impedance tomography (EIT) allows fascicular CAP with a resolution <200 µm, <1 ms using non-penetrating flexible nerve cuff electrode array. Here, we validate EIT rat sciatic comparison to micro-computed (microCT) and...
Abstract Spatially selective vagus nerve stimulation (sVNS) offers a promising approach for addressing heart disease with enhanced precision. Despite its therapeutic potential, VNS is limited by off‐target effects and the need time‐consuming titration. Our research aimed to determine spatial organization of cardiac afferent efferent fibres within pigs achieve targeted neuromodulation. Using trial‐and‐error sVNS in vivo ex micro‐computed tomography fascicle tracing, we found significant...
Verhoeff's iron hematoxylin (VIH) followed by lead citrate (LC) applied to epoxy thin sections stained the dense component of elastic fibers heavily and peripheral microfibrillar lightly in guinea pig trachea mouse testis fixed with a glutaraldehyde-osmium tetroxide sequence. This method large fimbriated beneath tracheal epithelium, small stacked aggregates thereof deep lamina propria, cartilage adventitia fibroelastic band trachea. Fibers fetus differed from those adult, especially...
Verhoeff's iron hematoxylin (VIH) applied to epoxy thin sections provides ultrastructural staining of selected sites including elastic fibers, ribosomes, heterochromatin in nuclei somatic cells, chromatin clumps germ cells and the nucleolonema ovoid body nucleolus primary spermatocytes. Granules rat parotid acinar show several patterns not otherwise easily recognized. Mucous droplets or granules goblet Paneth ileum stain. Staining defined polymers indicates that VIH reacts with polycations...
Abstract Objective : The main objective of this study was to investigate which injection pattern led the best imaging fascicular compound activity in fast neural EIT peripheral nerve using an external cylindrical 2 × 14-electrodes cuff. Specifically, addressed identification optimal and region reconstructed volume image fascicles. Approach effect three different measurement protocol features (transversal/longitudinal injection, drive electrode spacing, referencing configuration) over...
The lack of understanding fascicular organisation in peripheral nerves limits the potential vagus nerve stimulation therapy. Two promising methods may be employed to identify functional anatomy fascicles within nerve: fast neural electrical impedance tomography (EIT), and penetrating multi-electrode arrays (MEA). These could provide a means image compound action nerve. We compared ability localise fascicle activity between silicon shanks (SS) carbon fibre (CF) EIT, with micro-computed...
Abstract Despite detailed characterization of fascicular organization somatic nerves, the functional anatomy fascicles evident in human and large mammal cervical vagus nerve is unknown. The a prime target for intervention field electroceuticals due to its extensive distribution heart, larynx, lungs, abdominal viscera. However, current practice approved stimulation (VNS) technique stimulate entire nerve. This produces indiscriminate non-targeted effectors undesired side effects. Selective...
Abstract Background Previous research has revealed the logical mapping of fascicles in both human somatic and pig vagus nerves, but organization within nerve remains largely unknown. Understanding its fascicular arrangement would significantly advance our knowledge autonomic nervous system facilitate studies application selective stimulation to avoid off-target effects. The purpose this study was trace thoracic branches investigate their organization, analyze nerves histologically...
Abstract Vagus nerve stimulation (VNS) is a promising therapy for treatment of various conditions resistant to standard therapeutics. However, due the lack understanding fascicular organisation vagus nerve, VNS leads unwanted off-target effects. Micro-computed tomography (microCT) can be used trace fascicles from periphery and image anatomy. In this work we optimised microCT protocol rat sciatic subsequent pig nerves. After differential staining, optimal staining time was selected scanning...
Imaging of the compound action potential (CAP) in fascicles peripheral nerves could help avoid side effects neuromodulation by selective stimulation identified fascicles. Existing methods have low resolution, limited imaging depth, or are invasive. We propose fast neural electrical impedance tomography (EIT), which allows fascicular CAP with a high resolution ∼200 μm, <1 ms. This uses non-penetrating flexible cuff electrode array 14 circumferential electrodes. has been validated rat...
Vagus nerve stimulation (VNS) is a promising therapy for the treatment of conditions resistant to standard therapeutics. However, due lack understanding functional fascicular organisation cervical vagus nerve, VNS leads unwanted off‐target effects. Contrast‐enhanced micro‐computed tomography (microCT) was optimised provide rapid visualisation fascicles within in 3D with little distortion sample and minimal artefacts rat sciatic nerves. The purpose this work trace anatomy autonomic nerves...
Background and Objectives: Spatially-selective vagus nerve stimulation (sVNS) offers a promising solution to mitigate the off-target effects associated with traditional VNS, potentially serving as precise method for addressing chronic heart failure (HF) by specifically targeting efferent cardiac fibers. This approach holds potential enhance therapeutic outcomes avoiding eliminating current need time-consuming titration required optimal VNS. Recent studies have demonstrated independent...
This protocol describes the scanner setup, parameters required and reconstruction of microCT scans for imaging pig vagus nerves fascicles within them.
This protocol describes the preparation and staining process of pig vagus nerves for microCT scanning.