A5051874072- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Electrochemical Analysis and Applications
- Molecular Sensors and Ion Detection
- Solid-state spectroscopy and crystallography
- Analytical Chemistry and Sensors
- Supramolecular Chemistry and Complexes
- Photoreceptor and optogenetics research
- Marine Toxins and Detection Methods
- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Nanopore and Nanochannel Transport Studies
- stochastic dynamics and bifurcation
- Genetic Neurodegenerative Diseases
- Neurological disorders and treatments
- Luminescence and Fluorescent Materials
- Physiological and biochemical adaptations
University of Valparaíso
2014-2022
Washington University in St. Louis
2020-2022
University of Vermont
2022
Valparaiso University
2013-2019
University of Wisconsin–Madison
2019
University of Chile
2016
Centro Científico Tecnológico de Valparaíso
2014-2015
Colciencias
2015
Despite a growing number of ion channel genes implicated in hereditary ataxia, it remains unclear how mutations lead to loss-of-function or death cerebellar neurons. Mutations the gene KCNMA1, encoding α-subunit BK have emerged as responsible for variety neurological phenotypes. We describe mutation (BKG354S) child with congenital and progressive ataxia cognitive impairment. The selectivity filter dramatically reduced single-channel conductance selectivity. BKG354S trafficked normally...
In contrast to most voltage-gated ion channels, hyperpolarization- and cAMP gated (HCN) channels open on hyperpolarization. Structure-function studies show that the voltage-sensor of HCN are unique but mechanisms determine gating polarity remain poorly understood. All-atom molecular dynamics simulations (~20 μs) HCN1 channel under hyperpolarization reveals an initial downward movement S4 following transfer last charge, breaks into two sub-helices with lower sub-helix becoming parallel...
In neurosecretion, allosteric communication between voltage sensors and Ca
Large-conductance Ca2+- and voltage-activated K+ channel (BK) open probability is enhanced by depolarization, increasing Ca2+ concentration, or both. These stimuli activate modular voltage sensors that are allosterically coupled to gating. Here, we report a point mutation of phenylalanine (F380A) in the S6 transmembrane helix that, absence internal Ca2+, profoundly hinders opening while showing only minor effects on sensor active–resting equilibrium. Interpretation these results using an...
Allosteric interactions between the voltage-sensing domain (VSD), Ca2+-binding sites, and pore govern mammalian Ca2+- voltage-activated K+ (BK) channel opening. However, functional relevance of crosstalk mechanisms on BK gating is still debated. We examined energetic interaction Ca2+ binding VSD activation by investigating effects internal currents. Our results indicate that sensor occupancy has a strong impact through coordinated mechanism in which to single α-subunit affects all VSDs...
Mammals maintain homeostatic control of their body temperature. Therefore, these organisms are expected to have adaptations that confer the ability detect and react both self ambient Temperature-activated ion channels been discovered be primary molecular determinants thermosensation. The most representative group constitutes members transient receptor potential superfamily, TRP, which activated by either low or high temperatures covering whole range physiologically relevant temperatures....
The accessory β1 subunit modulates the Ca
The cytotoxicity of dialkylated lariat ethers has been previously attributed to their ionophoric properties. Herein, we provide evidence that these effects are due loss membrane integrity rather than ion transport, a finding with important implications for the future design synthetic ionophores.
Synthetic ion channels based on benzo(crown-ether) compounds have been previously reported to function as ion-selective in planar lipid bilayers, with hydrogen bonding networks implicated the formation of self-aggregated complexes. Herein, we report synthesis and characterization two new families compounds, termed monoacylated monoalkylated benzo(crown-ethers) (MABCE), both which lack bond donors. Depending length alkyl chain substituent size macrocycle, MABCE inhibit bacterial growth...
Abstract Allosteric interplays between voltage-sensing domains (VSD), Ca 2+ -binding sites, and the pore domain govern - voltage-activated K + (BK) channel opening. However, functional relevance of mechanisms crosstalk on BK gating is still debated. We examined energetic interaction binding VSD activation measuring analyzing effects internal channels currents. Our results indicate that sensors occupancy has a strong impact through coordinated mechanism in which to single α-subunit affects...