Glycerol and other polyols are used as osmoprotectants by many organisms. Several yeasts fungi can take up glycerol proton symport. To identify genes involved in active uptake Saccharomyces cerevisiae we screened a deletion mutant collection comprising 321 encoding proteins with 6 or more predicted transmembrane domains for impaired growth on medium. Deletion of STL1, which encodes member the sugar transporter family, eliminates transport. Stl1p is present plasma membrane S. during...

This work presents the application of a new framework for sustainability metrics to industrial processes, in particular, chemical processes. The an process can be evaluated using set three-dimensional (3D) indicators that represent all three dimensions sustainability: economic, environmental, and societal. four 3D proposed this worknamely, material intensity, energy potential risk, environmental impactare applicable wide range systems. first two are associated with operation. remaining...

Several microalgae species have been exploited due to their great biotechnological potential for the production of a range biomolecules that can be applied in large variety industrial sectors. However, major challenge processes is make them economically viable, through commercially valuable compounds. Most these compounds are accumulated inside cells, requiring efficient technologies extraction, recovery and purification. Recent improvements approaching physicochemical treatments (e.g.,...

Industrial production and commercialization of biodiesel from microalgae have become a good alternative to conventional feedstock. Microalgae show high growth rate carbon sequestration can be easily cultivate in fresh and/or marine water, using non-arable soil. This study aims analyze the technical economic feasibility Phaeodactylum tricornutum, an algae biomass scaled-up scenario, considering local reality prices available technologies. The model assumes 80,000 m3 cultivation, set bubble...

The world population is ageing, in particular the developed world, with a significant increase percentage of people above 60 years old. They represent segment that more vulnerable to adverse environmental conditions. Among them, indoor air quality one most relevant, as elders spend comparatively time indoors than younger generations. Furthermore, recent COVID-19 pandemic contributed immensely raising awareness importance breathing for human health and fact vector airborne infections...

Energy has been the engine of nations' development, and this driven mankind towards growing energy needs, in particular for transportation, agricultural industrial activities buildings. transportation is based on oil derived fuel, whereas buildings consists mainly electricity, which produced from fossil fuels, nuclear power and/or renewable sources, such as hydro solar. Agricultural use a combination fuels electric energy. To increase sustainability production efficient use, it urgent that...

This study assesses the environmental impacts of producing 1000 tinplate aerosol cans at a Portuguese packaging company using life cycle assessment (LCA) methodology. The inventory analysis is based on real industrial data collected from for foreground processes, complemented with literature background processes. Two energy scenarios are compared: current production setup, which relies electricity grid and 100% natural gas, an optimized renewable scenario powered entirely by photovoltaic...

Potential of Microalgae Scenedesmus obliquus Grown in Brewery Wastewater for Biodiesel Production Teresa M. Mata*, Ana C. Melo, Sonia Meireles, Adelio Mendes, Antonio A. Martins, Nidia S. Caetano LEPAE – Laboratory Process, Environmental and Energy Engineering, Faculty University Porto (FEUP), R. Dr. Roberto Frias S/N, 4200-465 Porto, Portugal Unicer Bebidas, SA, Leca do Balio, 4466-955, Matosinhos, Department Natural Sciences, Engineering Technology (FCNET), Oporto Lusophone University,...

This work evaluates the embodied carbon and energy of an industrial building construction with 6733 m² built area, following a LCA methodology, on gate-to-gate approach. For LCI it was used primary data electricity, water diesel fuel, directly measured at site, information about materials in architecture stability phases, obtained from specialty design documents, complemented secondary literature. Results show 508.57 kgCO2-eq/m² 4908.68 MJ/m2 for building, being main contributor.

aLEPABE – Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of University Porto (FEUP), R. Dr. Roberto Frias S/N, 4200-465 Porto, Portugal bDepartment Chemical School Engineering (ISEP), Polytechnic Institute (IPP), Antonio Bernardino de Almeida 4200-072 cDepartment Environmental Natural Sciences, Technology (FCNET), Oporto Lusophone University, Augusto Rosa, 24, 4000-098 PortugalPortugal *tmata@fe.up.pt

This work aims to evaluate the water footprint of microalgae cultivation in a closed pilot-scale multi-tubular photobioreactor, taking into account life cycle stages reactor construction and its operation for cultivation, on gate-to-gate approach. The data was obtained from real production conditions complemented with literature inventory databases. Results indicate that total lies range 2.4-6.8 m3/kg dry biomass, being PBR stage responsible largest contribution (> 60 %), mainly due...

The increase in the world's energy demand has contributed to emergence of new sustainable sources, such as microalgae, with their great potential provide biofuels and other high value co-products for food health's markets. However, current biorefinery methodologies are either too complex extract targeted components high-value products, or require solvents toxicity humans environment. This work aims evaluate different lipid extraction approaches applied three microalgal species: Chlorella...