- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Microbial bioremediation and biosurfactants
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Aquatic Ecosystems and Phytoplankton Dynamics
- Oil Spill Detection and Mitigation
- Enzyme Production and Characterization
- Aquatic Ecosystems and Biodiversity
- RNA Interference and Gene Delivery
- Microbial Fuel Cells and Bioremediation
- Metabolomics and Mass Spectrometry Studies
- Fungal and yeast genetics research
- biodegradable polymer synthesis and properties
- Marine Toxins and Detection Methods
- Biochemical and biochemical processes
- Methane Hydrates and Related Phenomena
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Machine Learning and Data Classification
- Enzyme-mediated dye degradation
- Marine and coastal ecosystems
- Toxic Organic Pollutants Impact
- Membrane Separation Technologies
- Algal biology and biofuel production
University of Lisbon
2014-2025
Institute for Biotechnology and Bioengineering
2014-2025
National Laboratory of Energy and Geology
2014-2022
Instituto Superior Técnico
2020-2022
Aalborg University
2022
IIT@MIT
2014
National Institute of Health Dr. Ricardo Jorge
2008
Itaconic acid (IA), or 2-methylenesuccinic acid, has a broad spectrum of applications in the biopolymer industry owing to presence one vinyl bond and two groups structure. Its polymerization can follow similar mechanism as acrylic but additional functionality be incorporated into extra beta group. Currently, bio-based production IA relies on fermentation filamentous fungus Aspergillus terreus. However, difficulties associated with undertaken by fungi together pathogenic potential A. terreus...
Mannosylerythritol lipids (MEL) are glycolipids with unique biosurfactant properties and produced by Pseudozyma spp. from different substrates, preferably vegetable oils, but also sugars, glycerol or hydrocarbons. However, solvent intensive downstream processing the relatively high prices of raw materials currently used for MEL production drawbacks in its sustainable commercial deployment. The present work aims to demonstrate cellulosic investigate requirements consequences combining...
Biosurfactants can replace fossil-driven surfactants with positive environmental impacts, owing to their low eco-toxicity and high biodegradability. However, large-scale production application are restricted by costs. Such costs be reduced using renewable raw materials facilitated downstream processing. Here, a novel strategy for mannosylerythritol lipid (MEL) explores the combination of hydrophilic hydrophobic carbon sources sideways processing strategy, based on nanofiltration technology....
The presence of toxic compounds in lignocellulosic hydrolysates (LCH) is among the main barriers affecting efficiency lignocellulose-based fermentation processes, particular, to produce biofuels, hindering production intracellular lipids by oleaginous yeasts. These microbial oils are promising sustainable alternatives vegetable for biodiesel production. In this study, we explored adaptive laboratory evolution (ALE), under methanol- and high glycerol concentration-induced selective pressures,...
Synthetic oil spill dispersants have become essential in offshore response strategies. However, their use raises significant concerns regarding toxicity to phyto- and zooplankton other marine organisms, especially isolated vulnerable areas such as the Arctic shorelines. Sustainable alternatives may be developed by replacing major active components of commercial with natural counterparts. During this study, interfacial properties different types glycolipid-based biosurfactants (rhamnolipids,...
Glycolipid biosurfactants are the most prominent group of microbial biosurfactants, comprising rhamnolipids, sophorolipids and mannosylerythritol lipids (MELs). Usually, large amounts hydrophobic substrates (e.g., vegetable oils) used to achieve high titers (~200 g/L) a crude product low purity at values limited 50–60%, contaminated with unconsumed triacylglycerol residual free fatty acids monoacylglycerides. The methods reported for removal these contaminants use mixture organic solvents,...
Mannosylerythritol lipids (MELs), one of the most promising biosurfactants (BS), are glycolipids produced by yeasts or fungi, with great environmental performance and a high compatibility human body. MELs besides working as typical surfactants, can form diverse structures when in critical aggregation concentration (CAC), reducing surface tension water other solutions, being stable over wide range conditions. Among others, present anti-microbial, antitumor, antioxidant anti-inflammatory...
Fermentations are complex and often unpredictable processes. However, fermentation-based bioprocesses generate large volumes of data that currently underexplored. These can be used to develop data-driven models, such as machine learning (ML), improve process predictability. Among various fermentation products, biosurfactants have emerged promising candidates for several industrial applications. Nevertheless, biosurfactant large-scale production is not yet cost-effective. This study aims...
Fermentations are complex and often unpredictable processes. However, fermentation-based bioprocesses generate large volumes of data that currently underexplored. These can be used to develop data-driven models, such as machine learning (ML) improve process predictability. Among various fermentation products, biosurfactants have emerged promising candidates for several industrial applications. Nevertheless, the large-scale production is not yet cost-effective. This study aims forecasting...
The increasing demand for sustainable, economical and environmentally friendly solutions has positioned microalgae as promising candidates in biotechnology, particularly food, feed, nutraceutical, pharmaceutical, biofuel bioremediation applications. This review explores the role of Nannochloropsis genus other marine oleaginous co-cultivation systems, highlighting their mechanisms interaction with various microorganisms potential biotechnological purposes. Case studies sp. co-cultures...
The increasing demand for sustainable, economical, and environmentally friendly solutions has positioned microalgae as promising candidates in biotechnology, particularly food, feed, nutraceutical, pharmaceutical, biofuel, bioremediation applications. This review explores the role of Nannochloropsis genus other marine oleaginous co-cultivation systems, highlighting their mechanisms interaction with various microorganisms potential biotechnological purposes. Case studies sp. co-cultures...
The discovery of itaconic acid as a product citric pyrolytic distillation in 1837 opened the possibility using it polymer building block. Itaconic acid, featuring two carboxylic acids and an unsaturated group, can potentially be used block several chemical syntheses, with particular emphasis on manufacture. elucidation biochemical pathways originating from first Aspergillus terreus and, recently, species Ustilago genus, has intensified diversified research focused microbe-based production,...
Research on biosurfactants (BS) is consistently increasing in the past years, driven from their beneficial attributes over chemical surfactants. Lactobacilli (LAB) and yeast BS producers could prevail pathogenic strains, owing to GRAS status, broaden end-applications toward food pharmaceutical industries. However, increased cost of production confers a bottleneck for industrial manufacture. Hence, exploitation agro-industrial waste by-products as fermentation supplements currently spotlight....
Abstract BACKGROUND Moesziomyces antarcticus is an efficient producer of mannosylerythritol lipids (MEL), a biosurfactant with wide range potential applications. The use lignocellulosic biomass can contribute to sustainable MEL production. While sugars (e.g. D‐glucose and D‐xylose) be converted MEL, the required pretreatment releases by‐products that are potentially inhibitory for yeasts. A design experiment (DoE) was performed evaluate effect furfural, acetate formate on M. their capacity...
Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, most the studies focusing on MELs high titre production have been relying in use vegetable oils impact sustainability process economy. Herein, we report for first time using produced from microalgae. The bio-oil was extracted
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 53:129-140 (2008) - DOI: https://doi.org/10.3354/ame01228 Special: 'Progress and Perspectives in Ecology: Highlights of SAME 10, Faro, Portugal, September 2007' Cyanobacterial blooms natural waters southern Portugal: a water management perspective Helena M. Galvão1,*, Margarida P. Reis1, Elisabete Valério2, Rita B....
Toxic cyanobacteria are common in Portuguese freshwaters and a cause of concern, given that exposure to subacute levels cyanobacterial toxins through drinking recreational water might have deleterious effects on human health. Since 1996 several laboratories been involved the screening cyanotoxins different freshwater bodies, some regularly basis but most sporadically basis. Here we present data phytoplankton communities microcystins production occurring six reservoirs located dry region...
Mannosylerythritol lipids (MELs), one of the most promising biosurfactants (BS), are glycolipids produced by yeasts or fungi, which have great environmental performance and high compatibility with human body. MELs, besides working as typical surfactants, can form diverse structures when at above critical aggregation concentration (CAC), reduce surface tension water other solutions, be stable over a wide range conditions. Among others, MELs present antimicrobial, antitumor, antioxidant...
Mannosylerythritol lipids (MELs) are a group of biosurfactants with wide range potential applications, due to their excellent tensioactive properties, biocompatibility, and biodegradability. One the envisioned uses for MELs is in bioremediation marine terrestrial environments. However, knowledge regarding ecotoxicity limited. The current costs production this biosurfactant too high make it competitive surfactant market. In an effort facilitate use purposes, using sea water medium formulation...
Abstract Background Enzymes involved in xylan hydrolysis have several industrial applications. Selection of efficient microbial hosts and scalable bioreaction operations can lower enzyme production costs contribute to their commercial deployment. This work aims at investigating the Moesziomyces aphidis yeast cultivation conditions that deliver maximal xylanase titres, yields productivities using low‐cost nitrogen (N) carbon (C) sources. Results NaNO 3 KNO supplementation improved 2.9‐...