A5019715571- RNA regulation and disease
- CRISPR and Genetic Engineering
- Biomedical and Engineering Education
- Scientific Computing and Data Management
- Retinal Development and Disorders
- Recommender Systems and Techniques
- Biomedical Text Mining and Ontologies
- Advanced biosensing and bioanalysis techniques
- Functional Brain Connectivity Studies
- Forensic Toxicology and Drug Analysis
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Biomedical Ethics and Regulation
Synthego (United States)
2018-2022
Menlo School
2020
University of California, San Diego
2014
Efficient and precise genome editing requires a fast, quantitative, inexpensive assay to assess genotype following editing. Here, we present ICE (Inference of CRISPR Edits), which enables robust analysis edits using Sanger data. proposes potential outcomes for with guide RNAs, then determines are supported by the data via regression. The algorithm is reproducible, it can be used analyze experiments within days after transfection. We also confirm that produces accurate estimates across...
Abstract Efficient precision genome editing requires a quick, quantitative, and inexpensive assay of outcomes. Here we present ICE (Inference C RISPR E dits), which enables robust analysis CRISPR edits using Sanger data. proposes potential outcomes for with guide RNAs (gRNAs) then determines are supported by the data via regression. Additionally, develop score called ICE-D (Discordance) that can provide information on large or unexpected edits. We empirically confirm through over 1,800...
Abstract Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target chromosomal translocations. To address this, we develop method for light-induced sgRNA termed CRISPRoff. Here show that inactivation ribonucleoprotein attenuates within cells allows titratable levels efficiency spatial...