Impact statement Methane oxidizing microbes play a key role in reducing the emission of this potent greenhouse gas to atmosphere. The known versatility recently discovered anaerobic Methylomirabilota methanotrophs is limited. Here, we report novel uncultured Methylomirabilis species, Candidatus iodofontis , with genetic potential iodate respiration from biofilm iodine‐rich cavern spring water. Star‐like cells resembling oxyfera were directly observed and high‐quality metagenome‐assembled...

Abstract Massive biofilms have been discovered in the cave of an iodine-rich former medicinal spring southern Germany. The completely cover walls and ceilings cave, giving rise to speculations about their metabolism. Here we report on first insights into structure function biofilm microbiota, combining geochemical, imaging molecular analytics. Stable isotope analysis indicated that thermogenic methane emerging served as important driver formation. undisturbed cavern atmosphere contained up...

Effluents from wastewater treatment plants (WWTPs) containing micro-organisms and residual nitrogen can stimulate nitrification in freshwater streams. We hypothesized that different ammonia-oxidizing (AOB) nitrite-oxidizing (NOB) bacteria present WWTP effluents differ their potential to colonize biofilms the receiving In an experimental approach, we monitored biofilm colonization by nitrifiers ammonium- or nitrite-fed microcosm flumes after inoculation with activated sludge. a field study,...

Groundwater ecosystems are typically poor in organic carbon and productivity sustaining a low standing stock of microbial biomass. In consequence, food webs oligotrophic groundwater hypothesized to be bottom-up controlled. To date, quantitative information on communities, web interactions, flow is relatively lacking comparison that surface waters. Studying shallow, porous alpine aquifer we collected data the numbers prokaryotes, virus-like particles heterotrophic nanoflagellates (HNFs),...

Dissolved organic matter (DOM) plays a pivotal role in aquatic ecosystems, influencing water quality, microbial activity, and carbon cycling. This study investigates the composition, sources, dynamics of DOM Berlin’s urban groundwater, focusing on its variability across aquifer layers implications for quality ecosystem health. Groundwater samples collected over three years (2021–2023) were analyzed using fluorescence spectroscopy excitation-emission matrices (EEMs). The...

Dispersal and colonization are important for the assembly biodiversity of microbial communities. While emigration as initial step dispersal has become increasingly understood in model bacterial biofilms, drivers complex biofilms remain elusive. We grew microcosms from natural surface water laminar turbulent flow, investigated patterns fluorescently labeled cells microbeads nascent mature biofilms. Settling occurred nonrandom spatial governed by interplay local flow biofilm topography. was...

The microbial monitoring of drinking water production systems is essential to assure quality and minimize possible risks. However, the comparative impact microbes from surrounding aquifer those established within wells on parameters remains poorly understood. High pressure jetting a routine method impede well clogging by fine sediments also biofilms. In present study, bacterial communities were investigated in system before, during, after hydraulic purging. Variations observed between...

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 76:243-255 (2016) - DOI: https://doi.org/10.3354/ame01781 FEATURE ARTICLE Organic contamination versus mineral properties: competing selective forces shaping bacterial community assembly in aquifer sediments Michael Grösbacher1, Carolin Spicher1, Anne Bayer2, Martin Obst3, Clemens Karwautz1, Giovanni Pilloni1,6,...

Rivers are the "tip of iceberg", with underlying groundwater being unseen freshwater majority. Microbial community composition and dynamics shallow ecosystems thus crucial, due to their potential impact on ecosystem processes functioning. In early summer late autumn, samples river water from 14 stations 45 wells were analyzed along a 300 km transect Mur River valley, Austrian alps flats at Slovenian border. The active total prokaryotic communities characterized using high-throughput gene...

Sampling groundwater biodiversity is difficult because of limited access and issues with species identification. Environmental DNA (eDNA) provides a viable alternative to traditional sampling approaches, however knowledge the abundance fate in hinders interpretation data from these environments. Groundwater environments are dark have lower oxygen concentrations microbial activity than surface waters. Consequently, assumptions about ecosystems may not apply groundwaters. Here, we test...

Biodiversity, the source of origin, and ecological roles fungi in groundwater are to this day a largely neglected field fungal freshwater ecology. We used DNA-based Illumina high-throughput sequence analysis both gene markers 5.8S internal transcribed spacers region 2 (ITS2), improving taxonomic classification. This study focused on river mycobiome along an altitudinal longitudinal transect pre-alpine valley Austria two seasons. Using Bayesian network modeling approaches, we identified...

Abstract Microbial biofilms occur in many shapes and different dimensions. In natural semi‐artificial caves they are forming pendulous structures of 10 cm more. this study a methane driven microbial community former medicinal spring was investigated. The habitat completely covered by massive snottites with wobbly, gelatinous appearance. By using fluorescence techniques combination confocal laser scanning microscopy the architecture these so far unknown examined. imaging approaches applied...

The Vjosa River and its tributaries represent a large dynamic river network characterized by near natural flow regime largely undisturbed hydromorphological dynamics. Due to the high connectivity (e.g. lack of dams regulation in main stem), is hotspot biodiversity may serve as an ecological reference system. self-purification potential ecosystem describes resistance resilience contamination (disturbances). In rivers, contaminants are attenuated degraded not only visible channels, but most...

Increasing urbanization puts pressure on urban subsurface temperatures and the impact groundwater quality human health. However, consequences of heat island phenomenon, i.e. anomalies due to lifestyle, ecosystems have rarely been addressed date. Of particular interest are microorganisms, who omnipresent in intimately involved cycling carbon nutrients, (im)mobilization metals, natural attenuation contaminants, providing essential ecosystem services. In framework research project...

Springs are vital water sources, and their vulnerability to environmental changes, particularly climate change, is of growing concern. The collaborative research project ECOSPRING, funded by the Austrian Academy Sciences, focuses on assessment microbial communities quality patterns in selected springs all over Austria. Here, we will focus understanding response hydrological events change. first aim characterize based temporal dynamics terms physical-chemical characteristics. A detailed...

Dispersal and colonization are important for the assembly biodiversity of microbial communities. While emigration as initial step dispersal has become increasingly understood in model bacterial biofilms, drivers complex biofilms remain elusive. We grew microcosms from natural surface water laminar turbulent flow, investigated patterns fluorescently labeled cells microbeads nascent mature biofilms. Settling occurred nonrandom spatial governed by interplay local flow biofilm topography. was...