A5034393430- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Vestibular and auditory disorders
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Nitric Oxide and Endothelin Effects
- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Hearing, Cochlea, Tinnitus, Genetics
- Neuropeptides and Animal Physiology
- Pain Mechanisms and Treatments
- Neuroscience and Neural Engineering
- Prenatal Substance Exposure Effects
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Nicotinic Acetylcholine Receptors Study
- Apelin-related biomedical research
- Nuclear Receptors and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Material Properties and Processing
- Motor Control and Adaptation
- Acupuncture Treatment Research Studies
- Food Allergy and Anaphylaxis Research
Tokyo University of Pharmacy and Life Sciences
2025
Juntendo University
2025
Hokkaido University
1993-2016
Sapporo Medical University
2001-2016
Hiroshima University
2016
Tokushima Prefectural General Education Center
2005
Hitachi (Japan)
1997
Climbing fiber (CF) synapse formation onto cerebellar Purkinje cells (PCs) is critically dependent on the synaptogenesis from parallel fibers (PFs), other input to PCs. Previous studies revealed that deletion of glutamate receptor delta2 subunit (GluRdelta2) gene results in persistent multiple CF innervation PCs with impaired PF synaptogenesis, whereas mutation metabotropic subtype 1 (mGluR1) causes normal synaptogenesis. We demonstrate atypical CF-mediated EPSCs (CF-EPSCs) slow rise times...
Organized synapse formation on to Purkinje cell (PC) dendrites by parallel fibers (PFs) and climbing (CFs) is crucial for cerebellar function. In PCs lacking glutamate receptor δ2 (GluRδ2), PF synapses are reduced in number, numerous free spines emerge, multiple CF innervation persists adulthood. the present study, we conducted anterograde immunohistochemical labelings investigate how CFs innervate PC under weakened synaptogenesis PFs. GluRδ2 knock-out mouse, were distributed molecular layer...
Significance Of the two distinguished features in synaptic wiring of cerebellar Purkinje cells (PCs), developmental process and underlying mechanism mono-innervation by single climbing fibers (CFs) are well understood. However, those segregated dendritic innervation CFs parallel (PFs) remain unknown. Here, we show that CF PF territories initially expand with enlargement region overlapping innervation, segregation occurs as a result elimination synapses from proximal dendrites. synapse is...
Vascular abnormalities are a major cause of postoperative complications in irradiated tissues. Endothelial cell dysfunction characterized by diminished endothelium-dependent relaxation may be involved. We examined the and morphology endothelium human cervical arteries.Irradiated arteries were taken from neck region patients who had radiation therapy. Arteries did not receive therapy used as controls. Endothelium-dependent to acetylcholine A23187 was impaired arteries. Norepinephrine-induced...
In early postnatal development, perisomatic innervation of cerebellar Purkinje cells (PCs) switches from glutamatergic climbing fibers (CFs) to GABAergic basket cell (BFs). Here we examined the switching process in C57BL/6 mice. At day 7 (P7), most synapses were formed by CFs on somatic spines. The density CF–spine peaked at P9, when pericellular nest around PCs was developed, and constituted 88% total synapses. Thereafter, dropped 63% P12, 6% P15, <1% P20, whereas BF increased...
Abstract Telencephalin (TLCN) is a cell adhesion molecule selectively expressed in the telencephalon of mammalian brain. The mutant mice lacking TLCN had no detectable abnormalities their neural development and synaptic structures. Ablation increased hippocampal long‐term potentiation its saturation level. mutation enhanced performance radial maze water‐finding tasks, learning tasks with appetitive reinforcers, but not contextual fear conditioning Morris water aversive stimuli for...
WE used silver-enhanced immunogold electron microscopy to reveal synaptic localization of the glutamate transporter EAAT4 in mouse cerebellar Purkinje cells (PCs). Gold—silver particles representing were densely localized on extra-junctional membrane, but not junctional membrane PC spines contact with parallel fiber or climbing terminals. No particle accumulations observed at inhibitory synapses formed cell body and dendritic shafts PCs. Therefore, is selectively targeted site excitatory...
Estrogen status is known to affect the incidence of cardiovascular disease. Experiments were designed prove influences in vivo estrogen manipulations on vascular hyperpolarization and relaxation mediated by endothelium-derived hyperpolarizing factor (EDHF), explore possible mechanism contributing altered EDHF responses estrogen-deficient states. Mesenteric arteries with intact endothelium isolated from sham-operated (control), ovariectomized (OVX), or OVX 17β-estradiol replacement (OVX + E2)...
Tardive dyskinesia involves involuntary movements of body parts and is often observed in individuals taking antipsychotics for extended periods. Initial treatment strategies include reducing medication dosage, switching medications, or using drugs to suppress symptoms. One the therapeutic targets tardive vesicular monoamine transporter-2 (VMAT-2, also known as solute carrier family 18 member A2 [SLC18A2]), which functions an energy-dependent transporter monoamines. The are used during...
Introduction Pyramidal-like projection neurons in the cerebral cortex exhibit layer-specific positioning of their cell bodies and target specific cortical regions with apical dendrites. Reeler mutant mice, which lack gene for reelin protein secreted by Cajal–Retzius cells have scattered throughout cortex, display relatively intact global local neuronal network connections compared wild-type mice. The irregular morphologies these cells, extend dendrites a neuron-disoriented direction, are...
Although injured axons usually do not regenerate in the adult CNS, parallel fibers (PFs) synaptic connections onto cerebellar Purkinje cells (PCs). In this study, we investigated role of GluD2 regenerative process after PF transection using GluD2-knock-out (KO) mice. All dendritic spines on distal dendrites were innervated by PFs sham-operated wild-type controls, whereas one-third devoid innervation GluD2-KO both genotypes, a steep drop number synapses occurred with reciprocal surge free...
The glutamatergic transmission system plays a key role in afferent and efferent pathways involved micturition. By situ hybridization combined with retrograde Fast Blue labeling, expression of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor (GluR-A to -D) N-methyl-D-aspartate (NMDA) (NR1 NR2A-D) subunit mRNAs were examined visceromotor somatomotor neurons the rat lumbosacral spinal cord. Parasympathetic preganglionic (PGNs) intermediolateral nucleus highly expressed...