- Marine Invertebrate Physiology and Ecology
- Genomics and Phylogenetic Studies
- Marine Ecology and Invasive Species
- Genetics, Bioinformatics, and Biomedical Research
- Protist diversity and phylogeny
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- Biomedical Text Mining and Ontologies
- Machine Learning in Bioinformatics
- Gene expression and cancer classification
- Developmental Biology and Gene Regulation
- Genomics and Rare Diseases
- Planarian Biology and Electrostimulation
- BRCA gene mutations in cancer
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Nutrition, Genetics, and Disease
- Advanced Proteomics Techniques and Applications
- Fractal and DNA sequence analysis
- Marine Sponges and Natural Products
- RNA modifications and cancer
- Coral and Marine Ecosystems Studies
- Connexins and lens biology
- Chromosomal and Genetic Variations
National Institutes of Health
2015-2024
National Human Genome Research Institute
2015-2024
Center for Human Genetics
1999
Cancer Genetics (United States)
1999
National Institute of Mental Health
1999
National Eye Institute
1999
University Hospitals of Cleveland
1999
Case Western Reserve University
1999
Johns Hopkins University
1990-1997
National Center for Biotechnology Information
1995-1997
The Base of the Animal Tree? identity most basal lineages animal kingdom evolutionary tree has long been contested. Ryan et al. (p. 10.1126/science.1242592 ; see Perspective by Rokas ) sequenced genome ctenophore warty comb jelly or sea walnut, Mnemiopsis leidyi , and conclude that ctenophores alone, not sponges clade consisting both cnidarians, are extant animals. results suggest a specific process likely occurred—including repeated gains loss mesoderm, expansion genes associated with cell...
The murine gene CHD1 (MmCHD1) was previously isolated in a search for proteins that bound DNA promoter element. presence of chromo (chromatin organization modifier) domains and an SNF2-related helicase/ATPase domain led to speculation this regulated chromatin structure or transcription. This study describes the cloning characterization three novel human genes related MmCHD1. Examination sequence databases produced several more genes, most which were not known be similar MmCHD1, yielding...
Hox genes were critical to many morphological innovations of bilaterian animals. However, early evolution remains obscure. Phylogenetic, developmental, and genomic analyses on the cnidarian sea anemone Nematostella vectensis challenge recent claims that code is a invention no "true" exist in phylum Cnidaria.
The abundant and highly-conserved nucleoprotelns comprising the high mobility group−1/−2 (HMG−1/−2) family contains two homologous basic domains of about 75 amino acids. These domains, termed HMG-1 boxes, are highly structured facilitate HMG-DNA interactions. Many proteins that regulate various cellular functions involving DNA binding whose target sequences share common structural characteristics have been identified as having an box; these include RNA polymer-ase I transcription factor UBF,...
The histone fold motif has previously been Identified as a structural feature common to all four core histones and is involved In both histone-histone histone- DNA Interactions. Through the use of novel searching method, group proteins containing established. in this are wide variety functions related mostly metabolism. Most these engage protein-protein or protein-DNA interactions, do their counterparts. Among these, CCAAT-specific transcription factor CBF its yeast homologue HAP two...
Cataracts are a significant public health problem. Here, we describe the genetic alteration responsible for progressive form of cataract, segregating as an autosomal dominant trait in three-generation pedigree. Unlike most cataracts, these not clinically apparent at birth but initially observed first year or two life. The opacification evolves relatively slowly, generally necessitating removal lens childhood early adolescence. A genome-wide search our kindred revealed linkage 2q33–35 where...
The much-debated phylogenetic relationships of the five early branching metazoan lineages (Bilateria, Cnidaria, Ctenophora, Placozoa and Porifera) are fundamental importance in piecing together events that occurred animal evolution. Comparisons gene content between organismal have been identified as a potentially useful methodology for reconstruction. However, these comparisons require complete genomes that, until now, did not exist ctenophore lineage. homeobox superfamily genes is...
Abstract Background Calcium-activated photoproteins are luciferase variants found in photocyte cells of bioluminescent jellyfish (Phylum Cnidaria) and comb jellies Ctenophora). The complete genomic sequence from the ctenophore Mnemiopsis leidyi , a representative earliest branch animals that emit light, provided an opportunity to examine genome organism uses this class for bioluminescence look genes involved light reception. To determine when photoprotein first arose, we examined other...
Abstract Retroviruses integrate into the host genome in patterns specific to each virus. Understanding causes of these can provide insight viral integration mechanisms, pathology and evolution, is critical development safe gene therapy vectors. We generated murine leukemia virus integrations human HepG2 K562 cells subjected them second-generation sequencing, using a DNA barcoding technique that allowed us quantify independent events. characterized >3 700 000 unique events two...
Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How fate is induced in these animals, and whether this process related bilaterian embryonic germline induction, unknown. We show transcription factor AP2 (Tfap2), regulator of mammalian lines, acts commit cells, known as i-cells, the cell clonal cnidarian
The epithelial and interstitial stem cells of the freshwater polyp
Cell fate stability is essential to maintaining "law and order" in complex animals. However, high comes at the cost of reduced plasticity and, by extension, poor regenerative ability. This evolutionary trade-off has resulted most modern animals being rather simple or non-regenerative. The mechanisms mediating cellular allowing for regeneration remain unknown. We show that signals emitted senescent cells can destabilize differentiated state neighboring somatic cells, reprogramming them into...
Hirschsprung disease (HSCR) is a multigenic neurocristopathy clinically recognized by aganglionosis of the distal gastrointestinal tract. Patients presenting with in association hypopigmentation are classified as Waardenburg syndrome type 4 (Waardenburg-Shah, WS4). Variability phenotype WS4 patients equivalent mutations suggests influence genetic modifier loci this disorder. Sox10(Dom)/+ mice exhibit variability and influenced background similar to that observed patients. We have constructed...
Abstract Background Intercellular signaling pathways are a fundamental component of the integrating cellular behavior required for evolution multicellularity. The genomes three four early branching animal phyla (Cnidaria, Placozoa and Porifera) have been surveyed key components, but not fourth (Ctenophora). Genomic data from ctenophores could be particularly relevant, as proposed to one earliest metazoan phyla. Results A preliminary assembly lobate ctenophore Mnemiopsis leidyi genome...
<i>Background:</i> Advances in technology have made individual access to personal genetic information foreseeable the near future. Policy makers and media forecast that ready availability of profiles would benefit both health care system by improving outcomes decreasing cost. However, there is a significant gap between having data either wanting or understanding it provides. <i>Objective:</i> Our primary aim was evaluate, using population-based sample healthy adults,...
The TGF-β signaling pathway is a metazoan-specific intercellular known to be important in many developmental and cellular processes wide variety of animals. We investigated the complexity possible functions this member one earliest branching metazoan phyla, ctenophore Mnemiopsis leidyi. A search recently sequenced genome revealed an inventory genes encoding ligands rest components superfamily pathway. contains nine ligands, two TGF-β-like family members, BMP-like five gene products that were...
Recent advances in sequencing technology have led to a rapid accumulation of mitochondrial DNA (mtDNA) sequences, which now represent the wide spectrum animal diversity. However, one phylum—Ctenophora—has, date, remained completely unsampled. Ctenophores, small group marine animals, are interest due their unusual biology, controversial phylogenetic position, and devastating impact as invasive species. Using data from Mnemiopsis leidyi genome project, we Polymerase Chain Reaction (PCR)...