A5060012394- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- bioluminescence and chemiluminescence research
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Phytase and its Applications
- Enzyme Production and Characterization
- Bacillus and Francisella bacterial research
- Redox biology and oxidative stress
- Antibiotic Resistance in Bacteria
- Cassava research and cyanide
- Environmental Toxicology and Ecotoxicology
Georgetown University
2018-2020
Bradley University
2016
Staphylococcus aureus requires branched-chain amino acids (BCAAs; isoleucine, leucine, valine) for protein synthesis, fatty acid and environmental adaptation by responding to their availability via the global transcriptional regulator CodY. The importance of BCAAs S. physiology necessitates that it either synthesize them or scavenge from environment. Indeed uses specialized transporters BCAAs, however, its ability has remained conflicted reports is auxotrophic leucine valine despite carrying...
In Staphylococcus aureus, the transcription factor CodY modulates expression of hundreds genes, including most virulence factors, in response to availability key nutrients like GTP and branched-chain amino acids. Despite numerous studies examining how controls gene directly or indirectly, virtually nothing is known about extent which exerts its effect through small regulatory RNAs (sRNAs). Herein, we report first set sRNAs under control CodY. We reveal that staphylococcal sRNA RsaD...
ABSTRACT In Staphylococcus aureus , the global transcriptional regulator CodY modulates expression of hundreds genes in response to availability GTP and branched-chain amino acids isoleucine, leucine, valine (ILV). DNA-binding activity is high when ILV are abundant. When limited, CodY's affinity for DNA drops, altering CodY-regulated targets. this work, we investigated impact guanine nucleotides (GNs) on S. physiology by constructing a guaA null mutant (Δ strain). De novo biosynthesis...
ABSTRACT Organisms growing aerobically generate reactive oxygen-containing molecules, such as hydrogen peroxide (H 2 O ). These oxygen molecules damage enzymes and DNA may even cause cell death. In response, Bacillus subtilis produces at least nine potential peroxide-scavenging enzymes, two of which appear to be the primary responsible for detoxifying peroxides during vegetative growth: a catalase (encoded by katA ) an alkylhydroperoxide reductase (Ahp, encoded ahpC AhpC uses redox-active...
Organisms growing aerobically generate reactive oxygen species such as hydrogen peroxide. These molecules damage enzymes and DNA, potentially causing cell death. In response, Bacillus subtilis produces at least nine potential peroxide-scavenging enzymes; two belong to the alkylhydroperoxide reductase (Ahp) class of peroxidases. Here, we explore role one these Ahp homologs, AhpA. While previous studies demonstrated that AhpA can scavenge peroxides thus defend cells against peroxides, they did...