A5069637366- Mast cells and histamine
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Veterinary Oncology Research
- Bone health and treatments
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Complement system in diseases
- Bone Metabolism and Diseases
- Bone fractures and treatments
- dental development and anomalies
- Angiogenesis and VEGF in Cancer
- Infectious Diseases and Mycology
- Shoulder Injury and Treatment
- Fibroblast Growth Factor Research
- Immune Response and Inflammation
- Shoulder and Clavicle Injuries
- Nerve Injury and Rehabilitation
- Cancer-related gene regulation
- Neonatal Respiratory Health Research
- Proteoglycans and glycosaminoglycans research
- Salivary Gland Disorders and Functions
- Genetic Syndromes and Imprinting
Nippon Veterinary and Life Science University
2019-2023
Showa University
2011-2017
Showa University Hospital
2017
Showa University Fujigaoka Hospital
1999-2001
The presence of macrophages in dental pulp is well known. However, whether these proliferate and differentiate the situ, or they constantly migrate from blood stream into remains unknown. We have examined compared development an organ culture system with vivo tooth organs to clarify developmental mechanism macrophages. first mandibular molar ICR mice aged between 16 days gestation (E16) 5 postnatally were used for experiments. Those E16 cultured up 14 without 10% fetal bovine serum. Dental...
Although it is known that osteoclasts are multinucleated cells responsible for bone resorption, the mechanism by which their size regulated unclear. We previously reported an actin‐rich superstructure, termed zipper‐like structure, specifically appears during fusion of large osteoclast‐like (OCLs). Actin cytoskeleton reorganization in a signaling network includes macrophage colony‐stimulating factor (M‐CSF) receptor, proto‐oncogene, Src, and small GTPases. Here, we examined role actin...
ABSTRACT The aim of this study was to elucidate the role zipper-like structure (ZLS), a podosome-related that transiently appears at cell contact zone, in osteoclast fusion. Live-cell imaging osteoclasts derived from RAW264.7 cells transfected with EGFP-actin revealed consistent symmetrical retrograde actin flow ZLS, but not podosome cluster, ring or belt. Confocal showed distributions F-actin, vinculin, paxillin and zyxin ZLS were different those Thick filament bundles running outside...
Fractures are common traumatic injuries that mainly occur in the metaphyses of long bones such as proximal humerus, distal radius, and femur. However, most studies fracture repair processes have focused on diaphyseal region. In this study, we compared bone metaphysis diaphysis mouse tibia. Bone apertures were formed tibial diaphysis. At indicated times after surgery, samples collected, healing process was investigated using micro-computed tomography, well histological, immunohistochemical,...
Abstract Tooth agenesis is described as the absence of one or more teeth. It caused by a failure in tooth development and most common human developmental anomalies. We herein report genomic analyses selective mandibular incisor (SMIA) using exome sequencing. Two Japanese families with SMIA were subjected to sequencing, family sequence similarity 65 member A ( FAM65 ), nuclear factor activated T-cells 3 NFATC3 ) cadherin-related 23 gene CDH23 detected. In follow-up study, 51 32 Korean...
Mammalian erythropoiesis can be divided into two distinct types, primitive and definitive, in which new cells are derived from the yolk sac hematopoietic stem cells, respectively. Primitive occurs within a restricted period during embryogenesis. erythrocytes remain nucleated, their hemoglobins different those definitive erythrocytes. Embryonic type hemoglobin is expressed adult animals under genetically abnormal condition, but its later expression has not been reported normal animals, even...
A 10-year-old female Papillon dog that had previously developed a mammary tumor was admitted for treatment of hypoglycemic attack. Blood examination showed severe hypoglycemia and decreased blood insulin concentration. Computed tomography indicated multiple tumors in the cranial caudal lobes right lung. These were resected surgically diagnosed as pulmonary adenocarcinomas by histopathologic examination. Hypoglycemia temporarily improved after resection, but event occurred 2 months surgery....
Abstract Histidine decarboxylase (HDC), histamine synthase, is expressed in hematopoietic stem cells and lineage‐committed progenitors the bone marrow (BM). However, role of hematopoiesis not well described. To evaluate hematopoiesis, we analyzed changes HDC expression at sites, BM, spleen, liver 2‐, 3‐, 6‐week‐old wild‐type mice. We also performed morphological analyses sites using HDC‐deficient (HDC‐KO) In adults, BM was higher than that spleen showed an age‐dependent increase....
We previously reported that the injection of nitrogen-containing bisphosphonate (NBP) induced site erythropoiesis to shift from bone marrow (BM) spleen. Our previous study established a severely anemic mouse model was treated with combination NBP phenylhydrazine (PHZ), which newly discovered hematopoietic organs in omentum. No reports have shown new form under any condition. characterized structures and factors related formation these organs. Splenectomized mice were inhibit BM then injected...
Vascular endothelial growth factor-A (VEGF-A) is a principal regulator of hematopoiesis as well angiogenesis. However, the functions VEGF-A and its receptors (VEGFRs) in differentiation mast cells (MCs) skin remain unclear. The aim this study was to determine expression patterns two VEGFRs (Flk1 Flt1) MCs during development maturation rats. From 17th days embryonic (E17) 1 day after birth (Day 1), most were immature containing predominant alcian blue (AB)+ rather than safranin O (SO)+...
Purpose: Our previous study indicated that nitrogen-containing bisphosphonate (NBP) induced the extramedullary erythropoiesis in spleen by depletion of bone marrow resident macrophages, which support erythropoiesis. In this study, we focused on liver as site hematopoiesis NBP-treated splenectomized mice. Methods: Splenectomized mice were treated with NBP (4-amino-1-hydroxybutylidene-1, 1-bisphosphonate, 40μmol/kg). At several times, livers and marrows analyzed histological,...
Introduction It is well established that multinucleated osteoclasts are the main cells to resorb calcified tissues. However, it still remains possibility mononuclear other than directly or destruct In this study, we examined ontogenic development of long bones in vivo and vitro process bone destruction during experimentally induced mouse arthritis model detect tissue by cells. Materials Methods Humeral from E16 Balb/c mice were dissected processed for transmission electron microscopy (TEM)....