A5005161996- Blood Coagulation and Thrombosis Mechanisms
- Mass Spectrometry Techniques and Applications
- Muscle Physiology and Disorders
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Hemophilia Treatment and Research
- Muscle activation and electromyography studies
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Proteoglycans and glycosaminoglycans research
- Microfluidic and Bio-sensing Technologies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cardiomyopathy and Myosin Studies
- Advanced Biosensing Techniques and Applications
- Protease and Inhibitor Mechanisms
- Isotope Analysis in Ecology
- Muscle metabolism and nutrition
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- HER2/EGFR in Cancer Research
- Metabolism and Genetic Disorders
- Blood properties and coagulation
GlaxoSmithKline (United States)
2022
South College
2022
University of Vermont
2005-2021
St Bartholomew's Hospital
2000
We hypothesize that age-related skeletal muscle dysfunction and physical disability may be partially explained by alterations in the function of myosin molecule. To test this hypothesis, at whole muscle, single fiber, molecular levels was measured young (21–35 yr) older (65–75 male female volunteers with similar activity levels. After adjusting for size, adults had knee extensor isometric torque values compared young, but lower isokinetic power, most notably women. At single-fiber levels,...
A classic problem in analytical chemistry has been determination of individual components a mixture without availability the pure components. Measurement distribution isotopomers labeled compound or compounds is an example this that commonly encountered when stable isotopically metabolites are used to determine vivo kinetics and metabolism. We present method uses measured mass spectral data unlabeled material represent any all combinations isotopomer variations abundances these isotopomers....
The effect of glycosylation on tissue factor (TF) activity was evaluated, and site-specific full-length recombinant TF (rTF) that natural from human placenta (pTF) were studied by liquid chromatography-tandem mass spectrometry. amidolytic the TF.factor VIIa (FVIIa) complex toward a fluorogenic substrate showed catalytic efficiency (V(max)) increased in order rTF(1-243) (Escherichia coli) < rTF(1-263) (Sf9 insect cells) pTF for glycosylated deglycosylated forms. Substrate hydrolysis unaltered...
Peptide identification by liquid chromatography–mass spectrometry (LC–MS) requires retention and elution of peptides from the LC column. Although medium hydrophobic are readily retained C18 columns that commonly used in proteomics, short hydrophilic not nor measured MS due to their void volume after sample injection. These nonretained can possess important post-translational modifications, such as glycosylation or phosphorylation. We describe a total LC–MS method employs reverse phase column...
Drug-load (DL) characterization of antibody-drug conjugates (ADCs) is an important analytical task due to its designation as a critical quality attribute (CQA) affecting potency and stability. Intact subunit liquid chromatography-mass spectrometry (LC-MS) analyses can determine global drug-to-antibody ratios (DARs) that correlate well with other orthogonal methods; however, peptide mapping chromatography-tandem mass (LC-MS/MS) analysis has struggled provide complementary site-specific...
The possibilities to directly couple different separation methods with mass spectrometry (MS) for the analysis of intact proteins has attracted significant attention over last decade. While sample preparation is critical MS analyses, coupling an efficient method before can significantly improve ability resolve protein heterogeneity and reveal masses or higher-order structure information (e.g., PTM conformation changes). To that end, this review focuses on common online techniques coupled...