A5066063513- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Calcium signaling and nucleotide metabolism
- Photosynthetic Processes and Mechanisms
- Toxin Mechanisms and Immunotoxins
- Endoplasmic Reticulum Stress and Disease
- Retinal Development and Disorders
- Kruppel-like factors research
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Genetic and Kidney Cyst Diseases
- Cancer-related gene regulation
- Lipid metabolism and biosynthesis
- PARP inhibition in cancer therapy
- Genetic Syndromes and Imprinting
- Adenosine and Purinergic Signaling
- Protist diversity and phylogeny
- Analytical Methods in Pharmaceuticals
- Plant nutrient uptake and metabolism
- Peroxisome Proliferator-Activated Receptors
- Click Chemistry and Applications
- Neonatal Health and Biochemistry
- Analytical Chemistry and Chromatography
- Structural and Chemical Analysis of Organic and Inorganic Compounds
National Research Council
2015-2025
Institute for Experimental Endocrinology and Oncology
2022-2025
Institute of Protein Biochemistry
2010-2020
Institute of Cell Biology and Neurobiology
2019
National Academies of Sciences, Engineering, and Medicine
2013-2015
Mario Negri Sud Foundation
1997-2012
In-Q-Tel
2012
Telethon Institute Of Genetics And Medicine
2009-2010
University of California, San Diego
1997-2003
Mario Negri Institute for Pharmacological Research
1999
Organelle inheritance is an essential feature of all eukaryotic cells. As with other organelles, the Golgi complex partitions between daughter cells through fission its membranes into numerous tubulovesicular fragments. We found that protein CtBP3/BARS (BARS) was responsible for driving during mitosis in vivo. Moreover, by vitro analysis, we identified two stages this fragmentation process: disassembly stacks a tubular network, and BARS-dependent these tubules. Finally, BARS-induced...
ABSTRACT In mammalian cells, the Golgi complex is composed of stacks that are connected by membranous tubules. During G2, disassembled into isolated stacks. This process required for entry mitosis, indicating correct inheritance organelle monitored a ‘Golgi mitotic checkpoint’. However, regulation and molecular mechanisms underlying this disassembly still poorly understood. Here, we show JNK2 has crucial role in G2-specific separation through phosphorylation Ser277 Golgi-stacking protein...
Incubation of permeabilized cells with mitotic extracts results in extensive fragmentation the pericentriolarly organized stacks cisternae. The fragmented Golgi membranes are subsequently dispersed from pericentriolar region. We have shown previously that this process requires cytosolic protein mitogen-activated kinase 1 (MEK1). Extracellular signal–regulated (ERK) and ERK2, known downstream targets MEK1, not required for (Acharya et al. 1998). now provide evidence MEK1 is specifically...
Abstract The Golgi apparatus is composed of stacks cisternae laterally connected by tubules to form a ribbon-like structure. At the onset mitosis, ribbon broken down into discrete stacks, which then undergo further fragmentation. This cleavage required for G2/M transition, thus indicates that ‘Golgi mitotic checkpoint’ couples inheritance with cell cycle transition. We previously showed Golgi-checkpoint regulates centrosomal recruitment kinase Aurora-A; however, how unlinking this was...
Brefeldin A (BFA) is a fungal metabolite that exerts profound and generally inhibitory actions on membrane transport. At least some of the BFA effects are due to inhibition GDP-GTP exchange ADP-ribosylation factor (ARF) catalyzed by protein(s). ARF activation likely be key event in association non-clathrin coat components, including itself, onto transport organelles. ARF, addition participating transport, known function as cofactor enzymatic activity cholera toxin, bacterial...
ADP-ribosylation is a posttranslational modification that modulates the functions of many target proteins. We previously showed fungal toxin brefeldin A (BFA) induces C-terminal–binding protein-1 short-form/BFA–ADP-ribosylation substrate (CtBP1-S/BARS), bifunctional protein with roles in nucleus as transcription factor and cytosol regulator membrane fission during intracellular trafficking mitotic partitioning Golgi complex. Here, we report CtBP1-S/BARS by BFA occurs via nonconventional...
Amitotically activated mitogen-activated protein kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells. We show that MEK1 is found on apparatus late prophase. The fragmented and dispersed membranes prometaphase later stages of mitosis do not contain MEK1. MEK1-dependent complex fragmentation through activation by RAF1 1. propose a RAF1-dependent its presence prophase required for fragmentation.
At the onset of mitosis, Golgi complex undergoes a multistep fragmentation process that is required for its correct partitioning into daughter cells. Inhibition this results in cell cycle arrest at G2 stage, suggesting inheritance monitored by "Golgi mitotic checkpoint." However, molecular basis block not known. Here, we show G2-specific stage concomitant with centrosome recruitment and activation kinase Aurora-A, an essential regulator entry mitosis. We impairs which progression....