A5090125523- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Adrenal Hormones and Disorders
- Birth, Development, and Health
- Blood Pressure and Hypertension Studies
- Extracellular vesicles in disease
- Adrenal and Paraganglionic Tumors
- Hormonal and reproductive studies
- Cardiovascular Disease and Adiposity
- Adipokines, Inflammation, and Metabolic Diseases
- Stress Responses and Cortisol
- Sodium Intake and Health
- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Estrogen and related hormone effects
- Adipose Tissue and Metabolism
- Neonatal Respiratory Health Research
- Pharmacogenetics and Drug Metabolism
- Nutritional Studies and Diet
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Apelin-related biomedical research
- Metabolism, Diabetes, and Cancer
- Eicosanoids and Hypertension Pharmacology
Pontificia Universidad Católica de Chile
2015-2025
Millennium Institute on Immunology and Immunotherapy
2014-2021
Millennium Institute
2014
University of Chile
1996
Blood pressure (BP) regulation is a complex process involving various hormones, including aldosterone and its mineralocorticoid receptor. Mineralocorticoid receptor expressed in several tissues, the kidney, plays crucial role regulating BP by controlling sodium water balance. During different stages of life, hormonal changes can affect activity levels, leading to BP. Increasing evidence suggests that sex steroids modulate levels. Estrogens, particularly estradiol, mediate biosynthesis...
Nurr1 is a transcription factor essential for the development of ventral dopaminergic neurons. In search regulatory mechanisms function, we identified SUMO (small ubiquitin-like modifier)-E3 ubiquitin-protein isopeptide ligase, PIASγ, as an interaction partner Nurr1. Overexpressed PIASγ and co-localize in nuclei transfected cells, their demonstrated through co-immunoprecipitation glutathione S-transferase pulldown assays. Co-expression with results potent repression Nurr1-dependent...
Classical apparent mineralocorticoid excess (AME) is a rare recessive disorder, caused by severe 11β-hydroxysteroid dehydrogenase type 2 enzyme (11β-HSD2) deficiency. AME manifests as low-renin pediatric hypertension, hypokalemia and high cortisol/cortisone (F/E) ratio. To evaluate nonclassic (NC-AME) due to partial 11β-HSD2 insufficiency its association with receptor (MR) activation, inflammatory parameters. Cross-sectional study. Primary care cohort. We recruited 127 adolescents adults....
The pathological hallmarks of Alzheimer's disease (AD) are amyloid-β (Aβ) plaques, neurofibrillary tangles, and glia activation. pathology also includes vascular amyloidosis cerebrovascular disease. Vascular compromise can result in hypoperfusion, local tissue hypoxia, acidosis. Activat ed microglia astrocytes phagocytose Aβ through membrane receptors that include scavenger receptors. Changes glial cells induced by extracellular acidosis could play a role the development AD. Here, we assess...
Abstract L ‐Arginine transport and nitric oxide (NO) synthesis ( ‐arginine/NO pathway) are stimulated by insulin, adenosine or elevated extracellular D ‐glucose in human umbilical vein endothelial cells (HUVEC). Adenosine uptake via the equilibrative nucleoside transporters 1 (hENT1) 2 (hENT2) has been proposed as a mechanism regulating plasma concentration, therefore its vascular effects veins. Thus, altered expression and/or activity of hENT1 hENT2 could lead to abnormal physiological...
Abstract Cortisol homeostasis is implicated in hypertension and metabolic syndrome. Two enzymes modulate cortisol availability; 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) preferentially converts inactive cortisone to cortisol, whereas 2 (11β-HSD2) cortisone. In contrast, 5α 5β reductases inactivate by conversion its tetrahydrometabolites: tetrahydrocortisol, allo-tetrahydrocortisol tetrahydrocortisone. A subtle local increase can be detected measuring 24-h urine metabolites, LC–MS/MS...
<title>Abstract</title> In obesity,white adipose tissue (WAT) undergoes hypertrophic and hyperplastic changes that are driven by phenotypical in preadipocytes adipocytes. WAT also causes a chronic inflammatory state modifies gene expression the secretome, including shedding of adipose-derived extracellular vesicles (AdEVs) into circulation, with these AdEVs affecting other cell types potentially modifying their phenotypes. <bold>Aim: </bold>To evaluate effects on renal endothelial cells...
Abstract Background Primary aldosteronism (PA) is the main cause of secondary arterial hypertension. In this study, we present medical treatment Hispanic patients with PA followed for up to 5 years, highlighting complete cure pharmacological in one our patients. Methods We studied 32 patients, every 6 months after starting MRA. A clinical response was normalization blood pressure (BP) absence other antihypertensive drugs. The biochemical considered potassium and renin. Responses were...
Nurr1 (NR4A2) is a transcription factor that belongs to the orphan NR4A group of nuclear receptor superfamily. plays key roles in origin and maintenance midbrain dopamine neurons, peripheral inflammatory processes. PIASγ, SUMO-E3 ligase, represses transcriptional activity. We report SUMOylated by SUMO-2 lysine 91 located activation function 1 domain Nurr1. SUMOylation markedly facilitated overexpressing wild type but not mutant form PIASγ lacking its first LXXLL motif (PIASγmut1). This...
High sodium intake has been associated with various noncommunicable disease like hypertension, cardiovascular disease, or stroke. To estimate accurately is challenging in clinical practice. We investigate the usefulness and limitations of assessing simultaneously by dietary assessment urinary samples both children adults. used a cross-sectional study design inviting 298 Chilean subjects (74 222 adults) aged between 9 66 years genders. Sodium was obtained from food composition data, based on...
Abstract Background The “nonclassic” apparent mineralocorticoid excess (NC-AME) has been identified in approximately 7% of general population. This phenotype is characterized by low plasma renin activity (PRA), high serum cortisol (F) to cortisone (E) ratio, cortisone, Fractional Excretion potassium (FEK) and normal-elevated systolic blood pressure (SBP). An early detection and/or identification novel biomarkers this could avoid the progression or future complications leading arterial...
Pathogenic variations in HSD11B2 gene triggers the apparent mineralocorticoid excess syndrome (AME). There is scarce information regarding phenotypes of subjects carrying heterozygous pathogenic variants gene. We investigated if serum cortisol/cortisone (F/E) ratio and cortisone are useful for identifying partial 11βHSD2 deficiency those subjects.We studied two patients diagnosed with AME their families either D223N or R213C mutation. also evaluated 32 healthy control (13 children 19 adults)...
Abstract High D ‐glucose reduces human equilibrative nucleoside transporter 1 (hENT1)‐mediated adenosine uptake involving endothelial nitric oxide synthase (eNOS), mitogen‐activated protein (MAP) kinase kinases and 2/MAP p42/44 (MEK/ERKs), C (PKC) activation in umbilical vein endothelium (HUVEC). Since NO represses SLC29A1 gene (hENT1) promoter activity we studied whether ‐glucose‐reduced hENT1‐adenosine transport results from lower expression HUVEC primary cultures. incubation (24 h) with...
To identify novel biomarkers associated with pediatric primary hypertension.We recruited 350 participants (4-16 years). Anthropometric parameters and aldosterone, plasma renin activity, cortisol, cortisone, Homeostasis Model Assessment Insulin Resistance (HOMA-IR), high-sensitivity C-reactive protein, adiponectin, IL-6, plasminogen activator inhibitor type 1 levels matrix metalloproteinase-9 metalloproteinase-2 (MMP-9 MMP-2) activities were measured. Genomic DNA was isolated. Patients...
Mineralocorticoid receptor (MR) activation within adipose tissue, triggers inflammation and metabolic syndrome development. The pharmacological blockade of MR provides beneficial effects for tissue. Our study evaluates the impact eplerenone implantation upon obesity. Experimental Approach: Mice with implanted placebo pellets were fed a normal diet (ND) or high fat (HFD). Additionally, group mice HFD an pellet. Metabolic biochemical parameters assessed in each animal group. Adipocyte size...
Primary aldosteronism (PA) represents 6% to 10% of all essential hypertension patients and is diagnosed using the aldosterone-to-renin ratio (ARR) confirmatory studies. The complexity PA diagnosis encourages identification novel biomarkers. Urinary extracellular vesicles (uEVs) are a potential source biomarkers, considering that their cargo reflects content parent cell.We aimed evaluate proteome uEVs from identify biomarker candidates for PA.Second morning spot urine was collected healthy...
Abstract BACKGROUND We aimed to study the associations of adipocytokines, endothelial damage markers, and high-sensitivity C-reactive protein (hs-CRP) with metabolic syndrome (MetS) components. METHODS This cross-sectional included 202 subjects categorized into MetS No-MetS according Harmonizing Adult Treatment Panel III. RESULTS Subjects showed higher levels proinflammatory molecules but significantly lower adiponectin than No-MetS. Among studied plasminogen activator inhibitor-1 (PAI-1)...
Abstract Background Familial hyperaldosteronism type I (FH-I) is caused by the unequal recombination between 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes, resulting in generation of a CYP11B1/B2 chimeric gene abnormal adrenal production. Affected patients usually show severe hypertension an elevated frequency stroke at young age. Aldosterone levels rise during pregnancy, yet pregnant women with FH-1, their hypertensive condition either remains unchanged or may even...
BACKGROUND Rac1 upregulation has been implicated in salt-sensitive hypertension as a modulator of mineralocorticoid receptor (MR) activity. could affect the expression oxidative stress markers, such hemoxigenase-1 (HO-1) or nuclear factor-B (NF-κB), and neutrophil gelatinase-associated lipocalin (NGAL), cytokine upregulated upon MR activation. AIM We evaluated RAC1 relation high salt intake association with MR, NGAL, HO-1, NF-κB expression, mineralo- glucocorticoids levels, inflammatory...