A5000186424- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Photoreceptor and optogenetics research
- Genetic Neurodegenerative Diseases
- Laser Applications in Dentistry and Medicine
- Autophagy in Disease and Therapy
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
- Cell death mechanisms and regulation
- Optical Imaging and Spectroscopy Techniques
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- interferon and immune responses
- Photosynthetic Processes and Mechanisms
- Advanced Proteomics Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Biotin and Related Studies
- Muscle Physiology and Disorders
- Cellular transport and secretion
- Sirtuins and Resveratrol in Medicine
- Parasitic infections in humans and animals
Wayne State University
2013-2025
The lineage relationship between short-lived effector T cells and long-lived memory is not fully understood. We have described T-GFP mice previously, in which naive early activated express GFP uniformly, whereas that differentiated into cytotoxic selectively lose expression. Here we studied antigen-specific CD8 cell differentiation using crossed to the TCR transgenic (Tg) P14 (specific for lymphocytic choriomeningitis virus glycoprotein peptide, gp33-41). After activation with antigenic...
Huntingtin interacting protein 14 (HIP14, ZDHHC17) is a huntingtin (HTT) with palmitoyl transferase activity. In order to interrogate the function of Hip14, we generated mice disruption in their Hip14 gene. Hip14-/- displayed behavioral, biochemical and neuropathological defects that are reminiscent Huntington disease (HD). Palmitoylation other HIP14 substrates, but not Htt, was reduced mice. dysfunctional presence mutant htt YAC128 mouse model HD, suggesting altered palmitoylation mediated...
Huntington disease (HD) is caused by polyglutamine expansion in the huntingtin (HTT) protein. Huntingtin-interacting protein 14 (HIP14), one of 23 DHHC domain-containing palmitoyl acyl transferases (PATs), binds to HTT and robustly palmitoylates at cysteine 214. Mutant exhibits reduced palmitoylation interaction with HIP14, contributing neuronal dysfunction associated HD. In this study, we confirmed that, among PATs, HIP14 its homolog DHHC-13 (HIP14L) are two major PATs that palmitoylate...
Cytochrome c (Cytc) is a multifunctional protein, acting as an electron carrier in the transport chain (ETC), where it shuttles electrons from bc1 complex to cytochrome oxidase (COX), and trigger of type II apoptosis when released mitochondria. We previously showed that Cytc regulated highly tissue-specific manner: isolated heart, liver, kidney phosphorylated on Y97, Y48, T28, respectively. Here, we have analyzed effect new phosphorylation site, threonine 58, which mapped rat by mass...
Cytochrome c (Cyt ) is a multifunctional protein that operates as an electron carrier in the mitochondrial transport chain and plays key role apoptosis. We have previously shown tissue‐specific phosphorylations of Cyt heart, liver, kidney play important regulation cellular respiration cell death. Here, we report purified from mammalian brain phosphorylated on S47 this phosphorylation lost during ischemia. characterized functional effects vitro using pig tissue recombinant phosphomimetic...
Prostate cancer is the second leading cause of cancer-related death in men. Two classic hallmarks are a metabolic switch from oxidative phosphorylation (OxPhos) to glycolysis, known as Warburg effect, and resistance cell death. Cytochrome c (Cytc) at intersection both pathways, it essential for electron transport mitochondrial respiration trigger intrinsic apoptosis when released mitochondria. However, its functional role has never been studied. Our data show that Cytc acetylated on lysine...
Cytochrome c (Cytc) is important for both mitochondrial respiration and apoptosis, of which are altered in cancer cells that switch to Warburg metabolism manage evade apoptosis. We earlier reported lysine 53 (K53) Cytc acetylated prostate cancer. K53 conserved mammals known be essential binding cytochrome oxidase apoptosis protease activating factor-1 (Apaf-1). Here we report the effects this acetylation on main functions by expressing acetylmimetic K53Q double knockout cells. Other variants...
Skeletal muscle is more resilient to ischemia-reperfusion injury than other organs. Tissue specific post-translational modifications of cytochrome c (Cytc) are involved in by regulating mitochondrial respiration and apoptosis. Here, we describe an acetylation site Cytc, lysine 39 (K39), which was mapped ischemic porcine skeletal removed sirtuin5 vitro. Using purified protein cellular double knockout models, show that K39 acetylmimetic K39Q replacement increases oxidase (COX) activity ROS...
It was previously reported that tyrosine 97 (Y97) of cytochrome c is phosphorylated in cow heart tissue under physiological conditions. Y97 phosphorylation shown to partially inhibit respiration vitro the reaction with purified oxidase. Here, we use phosphomimetic Y97E Cytc further characterize functional effects this modification both and cell culture models. In vitro, showed lower activity oxidase (COX), decreased caspase-3 activity, reduced rate reduction. Additionally, tended be...
The magnitude and diversity of Ag-specific T cell effector activity have been proposed to be controlled by an integration positive signals transduced the TCR negative originating from inhibitory surface molecules. Although lectin family NK cell-associated receptors has reported regulate function murine CTLs, gp49B1, Ig superfamily member is not known expressed on cells. Moreover, consequences lack endogenously receptor functions are known. We report that gp49B1 nearly all activated CD8 CD4...
We previously reported that serine-47 (S47) phosphorylation of cytochrome c (Cytc) in the brain results lower oxidase (COX) activity and caspase-3 vitro. here analyze effect S47 modification fibroblast cell lines stably expressing S47E phosphomimetic Cytc, unphosphorylated WT, or S47A Cytc. Our show Cytc partial inhibition mitochondrial respiration corresponding with membrane potentials (ΔΨm) reduced reactive oxygen species (ROS) production. When exposed to an oxygen-glucose...
The objective of the present study was to determine if combination alkaloids from Sophora moorcroftiana seeds and albendazole might be effective in treatment experimental echinococcosisin female NIH mice (6 weeks old weighing 18-20 g, N = 8 each group) infected withprotoscolices Echinococcus granulosus. Viable protoscolices (N 6 x 103) were cultured vitro 1640 medium mortality calculated daily. To vivo efficacy, inoculated intraperitoneally with viable then treated once daily by gavage for...
Abstract Background Brain injury is a leading cause of morbidity and mortality in patients resuscitated from cardiac arrest. Mitochondrial dysfunction contributes to brain following arrest; therefore, therapies that limit mitochondrial have the potential improve neurological outcomes. Generation reactive oxygen species (ROS) during ischemia–reperfusion critical component dependent on hyperactivation mitochondria resuscitation. Our previous studies provided evidence modulating function with...
Summary BNIP3 is a recently described pro‐apoptotic member of the Bcl‐2 family and in cDNA‐transfected cell lines, death occurs via caspase‐independent pathway with opening mitochondrial permeability transition (PT) pore rapid loss transmembrane potential (Δψ m ). However, its expression or function physiologic types not known. Our results using T‐cell receptor transgenic mice P14, specific for lymphocyte choreomeningitis virus (LCMV) glycoprotein, show that contrast to other molecules,...