A5003782759- Gene Regulatory Network Analysis
- Mosquito-borne diseases and control
- MicroRNA in disease regulation
- RNA Research and Splicing
- HIV Research and Treatment
- Photoreceptor and optogenetics research
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Protein Structure and Dynamics
- Cancer-related molecular mechanisms research
- Effects and risks of endocrine disrupting chemicals
- Advanced Fluorescence Microscopy Techniques
- Toxic Organic Pollutants Impact
- Cellular transport and secretion
- Molecular Biology Techniques and Applications
- Diffusion and Search Dynamics
- Molecular Communication and Nanonetworks
- Wnt/β-catenin signaling in development and cancer
- Chemical and Physical Studies
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Evolution and Genetic Dynamics
- RNA Interference and Gene Delivery
Heidelberg University
2008-2022
University Medical Centre Mannheim
2015
University Hospital Heidelberg
2015
German Cancer Research Center
2014-2015
DKFZ-ZMBH Alliance
2010-2011
National Academy of Sciences of Belarus
2003-2005
Institute of Biophysics and Cell Engineering
2003
A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA replicating virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate ultrastructural details RFs their relationship host cell organelles. Analyses human hepatic cells neural progenitor infected revealed endoplasmic reticulum (ER) membrane invaginations...
Stress granules (SGs) are formed in the cytosol as an acute response to environmental cues and activation of integrated stress (ISR), a central signaling pathway controlling protein synthesis. Using chronic virus infection model, we previously uncovered unique temporal control ISR resulting recurrent phases SG assembly disassembly. Here, elucidate molecular network generating this fluctuating by integrating quantitative experiments with mathematical modeling find that operates stochastic...
Adaptation of the chemotaxis sensory pathway bacterium Escherichia coli is integral for detecting chemicals over a wide range background concentrations, ultimately allowing cells to swim towards sources attractant and away from repellents. Its biochemical mechanism based on methylation demethylation chemoreceptors has long been known. Despite importance adaptation cell memory behavior, dynamics are difficult reconcile with current models precise adaptation. Here, we follow time courses...
The microRNA (miRNA) landscape changes during the progression of cancer. We defined a metastasis-associated miRNA using systematic approach. profiled and validated mRNA expression in unique series human colorectal metastasis tissues together with their matched primary tumors corresponding normal tissues. identified an exclusive signature that is differentially expressed metastases. Three these miRNAs were as key drivers EMT-regulating network acting though number novel targets. These targets...
// Olga Oleksiuk 1, 2, * , Mohammed Abba Kerem Can Tezcan 2 Wladimir Schaufler 3, 4 Felix Bestvater 3 Nitin Patil Udo Birk 5 Mathias Hafner 6 Peter Altevogt 7 Christoph Cremer 5, 8 Heike Allgayer 1 Department of Experimental Surgery, Medical Faculty Mannheim, University Heidelberg, Germany Centre for Biomedicine and Technology Light Microscopy Facility, German Cancer Research (DKFZ), Karlsruhe Institute Technology, University, Karlsruhe, Molecular Biology (IMB), Mainz, Cellular Biology,...
Abstract In the present work we studied, by chemiluminescence measurements, influence of lead on production reactive oxygen species (ROS) in haemolysates obtained from human erythrocytes incubated presence different concentrations acetate. Moreover, evaluated modification proteins and lipids erythrocyte lymphocyte membranes using fluorescence probes N ‐(1‐pyrene)maleimide (PM), laurdan pyrene. No significant changes were detected for with 1–10 µM acetate 3 h at 37 °C. By increasing...
Abstract Distant metastasis is the major determinant of patient outcome in colorectal cancer. By systematically analysing miR expression profiles resected metastasis-, corresponding primary tumor and normal tissues cancer patients, we were able to delineate a miR-signature indicative metastatically critical microRNA landscape. This “hot list” miRNAs, including established metastasis-related miRs like miR-34- let -7 families, as well rather novel miR-candidates miR-552, -218, -135, -210 -654,...
Members of the Flavivirus genus rely extensively on host cell endomembrane network to generate complex membranous replication organelles (ROs) that facilitate viral genome and production virus particles. For dengue Zika virus, these ROs included vesicles which are formed through membrane invagination into endoplasmic reticulum (ER) lumen, termed invaginated or vesicle packets (VPs), as well large areas bundled smooth ER, convoluted membranes. Though morphology virus-induced structures has...
Abstract We propose a novel approach for the detection of small non-coding RNAs in cells by Single Molecule Localization Microscopy (SMLM). describe modified and upgraded SMLM-setup apply this instrument first proof-of-principle concept to human cancer cell lines. Our method is able visualize single microRNA molecules fixed with localization accuracy 10-15 nm, quantify numbers analyse clustering particular subcellular sites, including exosomes. compared metastatic-site derived primary site...
Abstract Stress granules (SGs) are formed in the cytosol as an acute response to environmental cues and activation of integrated stress (ISR), a central signaling pathway controlling protein synthesis. Using chronic virus infection model, we previously uncovered unique temporal control ISR resulting recurrent phases SG assembly disassembly. Here, elucidate molecular network generating this fluctuating response, by integrating quantitative experiments with mathematical modeling, find that...