Fibroblast growth factor 23 (FGF23) is a hormone released primarily by osteocytes that regulates phosphate and vitamin D metabolism. Recent observational studies in humans suggest circulating FGF23 independently associated with cardiac hypertrophy increased mortality, but it unknown whether can directly alter function. We found significantly cardiomyocyte cell size vitro, the expression of gene markers hypertrophy, total protein content muscle. In addition, FGFR1 FGFR3 mRNA were most...

Fibroblast growth factor 23 (FGF23) is secreted primarily by osteocytes and regulates phosphate vitamin D metabolism. Elevated levels of FGF23 are clinically associated with endothelial dysfunction arterial stiffness in chronic kidney disease (CKD) patients; however, the direct effects on function unknown. We hypothesized that directly impairs vasorelaxation hindering nitric oxide (NO) bioavailability. detected expression all four subtypes FGF receptors (Fgfr1-4) male mouse aortas. Exogenous...

Aging is associated with insulin resistance and decreased insulin-stimulated glucose uptake into skeletal muscle. Although the mechanisms underlying age-related are not clearly defined, impaired defense against inflammation tissue oxidative stress likely causes. Heat shock proteins (HSPs) have been shown to protect from inhibit activation of kinases such as JNK, known interfere signaling pathway. While induction HSPs via chronic heat treatment has muscle obesity-related resistance, ability...

Heat shock proteins (HSPs) are chaperones that known to have important roles in facilitating protein synthesis, assembly and cellular protection. While HSPs be induced by damaging exercise, little is about how actually mediate skeletal muscle adaption exercise. The purpose of this study was determine the effects a heat pretreatment ensuing increase HSP expression on early remodeling signaling (2 48 h) events soleus (Sol) following bout downhill running. Male Wistar rats (10 weeks old) were...

Skeletal muscle dysfunction accompanies the clinical disorders of chronic kidney disease (CKD) and hereditary hypophosphatemic rickets. In both disorders, fibroblast growth factor 23 (FGF23), a bone-derived hormone regulating phosphate vitamin D metabolism, becomes chronically elevated. FGF23 has been shown to play direct role in cardiac dysfunction; however, it is unknown whether signaling can also directly induce skeletal dysfunction. We found expression potential receptors ( Fgfr1-4)...

Kulik, JR, Touchberry, CD, Kawamori, N, Blumert, PA, Crum, AJ, and Haff, GG. Supplemental carbohydrate ingestion does not improve performance of high-intensity resistance training. J Strength Cond Res 22: 1101-1107, 2008-The effects supplemental (CHO) on the squats to exhaustion (STE) were investigated with eight resistance-trained men. Subjects participated in a randomized, counterbalanced, double-blind, placebo-controlled protocol testing separated by 7 days. consumed 0.3g·kgCHO·bodymass−1...

We have recently reported that left atrial injections of the thromboxane A₂ (TXA₂) mimetic, (5<i>Z</i>)-7-[(1<i>R</i>,4<i>S</i>,5<i>S</i>,6<i>R</i>)-6-[(1<i>E</i>,3<i>S</i>)-3-hydroxy-1-octenyl]-2 -oxabicyclo[2.2.1]hept-5-yl]-5-heptenoic acid (U46619), induced ventricular arrhythmias in anesthetized rabbit. Data from this study led us to hypothesize TXA₂ may be inducing direct actions on myocardium induce these arrhythmias. The aim was further elucidate...

Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is the most recently identified phosphoinositide, and its functions have yet to be fully elucidated. Recently, members of our muscle group shown that PI(3,5)P2 plays an important role in skeletal function by altering Ca2+ homeostasis. Therefore, we hypothesized may also modulate cardiac contractility intracellular ([Ca2+]i) myocytes. We first confirmed was present increased insulin treatment cardiomyocytes via immunohistochemistry. To examine...

Autosomal recessive hypophosphatemic rickets (ARHR) is a heritable disorder characterized by hypophosphatemia, osteomalacia, and poor bone development. ARHR results from inactivating mutations in the DMP1 gene with human phenotype being recapitulated Dmp1 null mouse model which displays elevated plasma fibroblast growth factor 23. While has been well characterized, it not known what effects may also have on skeletal, cardiac, or vascular smooth muscle function, critical to understand treat...

The effects of resistance exercise on fiber-type-specific expression insulin-like growth factor I receptor (IGF-1R) and glucose transporter 4 (GLUT4) was determined in 6 healthy males. both genes increased Type fibers (p < 0.05), but only GLUT4 0.05) II fibers. These data demonstrates that an acute bout can up-regulate mechanisms uptake slow fast-twitch fibers, the IGF signaling axis may not be as effective

We have previously shown that the thromboxane (TXA2) receptor agonist, U46619, can directly induce ventricular arrhythmias were associated with increases in intracellular calcium cardiomyocytes. Since TXA2 is an inflammatory mediator and induces direct changes cardiomyocytes, we hypothesized released during ischemia or inflammation could also cause cardiac remodeling. U46619 (0.1-10 μM) was applied to isolated adult mouse primary muscle strips, cultured HL-1 cardiomyocytes markers of...

Vardiman, JP, Riggs, CE, Galloway, DL, Waxman, MB, Touchberry, CD, and Gallagher, PM. Salivary IgA is not a reliable indicator of upper respiratory infection in collegiate female soccer athletes. J Strength Cond Res 25(7): 1937-1942, 2011—It has been shown that mucosal immunity measures such as salivary immunoglobulin A (s-IgA) can be affected by sport activities resulted an increased susceptibility to infection. However, there limited research evaluated the change s-IgA throughout full...

ABSTRACT Introduction α7β1 integrin links the extracellular matrix to focal adhesion (FA) in skeletal muscle and serves as a stabilizing signal relayer. Heat shock (HS) induces expression of proteins that interact with FA. Methods Male Wistar rats were assigned 1 3 groups: control (CON); eccentric exercise (EE); or EE+HS (HS). Soleus was analyzed at 2 h 48 post‐exercise. Results The 120‐kDa α7 decreased EE HS groups, 70‐kDa peptide group Total kinase (FAK) RhoA Expression phosphorylated FAK...

Context: Therapeutic modalities that can increase intramuscular temperature commonly are used to treat injuries in the clinical setting. Researchers recently have suggested physiologic changes occurring during an also could provide a cytoprotective effect for exercise-induced muscle damage. Objective(s): To determine if Fluidotherapy treatment increases inducible expression of heat shock protein (HSP), identify rate heating occurs lower extremity with treatment, and evaluate relationship...

Forskolin (FSK) is capable of both stimulating and inhibiting the intracellular signaling pathways protein synthesis tissues other than skeletal muscle. The purpose this investigation was to determine if FSK administration affects various elements kinase B (Akt)–mammalian target rapamycin (mTOR) pathway in human Ten (n = 10) healthy, young (21.6 ± 1.3 years), nonobese (body mass index 25.5 3.5 kg·m –2 ), recreationally active males were selected for participation. Following an 8 h fast, 2...

Hyperthermia is an important approach for the treatment of several diseases. also thought to induce hypertrophy skeletal muscles in vitro and vivo, has been used as a therapeutic tool millennia. In first part our work, we revise relevant patents related utilization hyperthermia diagnostic human second part, present exciting new data on effects forced natural overexpression HSP72, using murine (muscle cells) ex vivo (primary muscles) models. These studies help demonstrate that are...

The purpose of this study was to examine the interaction amino acid supplementation and resistance exercise on Akt p70s6k phosphorylation in skeletal muscle. Four groups healthy college-aged males (N=5/group) volunteered for investigation: Branched-chain with (AA+EX), only (AA), Placebo (PCBO+EX), (PCBO). diet activity level subjects controlled 24 hours prior each trial. Three muscle biopsies (pre (T1), 30 minutes (T2) 4 (T3) post ingestion supplementation) were obtained during samples...

We hypothesize that bone acts as an endocrine organ by sending regulatory signals to skeletal, smooth, and cardiac muscles reciprocally muscle regulates mass strength independent of mechanical loading. To test this hypothesis we measured in vitro contractility function intact aortas, cerebral arteries, hearts, skeletal muscles, determined tibial trabecular cortical BV/TV a mouse lacking specific phosphatase (MIP/MTMR14; MIPKO). Systemic ablation MIP induces intracellular elevation PI(3,5)P2...

MSM is known to provide anti‐inflammatory and anti‐oxidant effects in both animals humans. Because exercise chronic disease have the potential induce inflammation oxidative stress skeletal muscle, may be a novel therapy counter negative consequences of these stressors on muscle function. Recent evidence has shown that supplementation human subjects can increase serum concentrations 2–3 mM. These been associated with reduced pain markers damage (creatine kinase) following damaging exercise....

We have shown that the inflammatory mediator thromboxane A2 (TxA2) can induce ventricular arrhythmias were associated with increased intracellular calcium in cardiomyocytes. able to prevent both and changes by inhibiting inositol trisphosphate (IP3) signaling. In this investigation, we sought determine if TxA2 receptor (TxA2R) activation would trigger apoptosis isolated adult mouse Our hypothesis is TxA2R‐stimulation induces IP3 formation subsequent triggers apoptosis. found TxA2Rs expressed...

Heat shock proteins are cytoprotective and have recently been shown to be involved in the repair process of myofibrils. PURPOSE: Thus, purpose this investigation was determine whether short wave diathermy can increase heat protein 70 (Hsp70) content skeletal muscle. METHODS: Eight subjects (6 females 2 males, 18-35 yrs) received a muscle biopsy from v. lateralis one leg then underwent 20-minutes followed by 20 minutes hot pack heating on other leg. Twenty-four hours following session,...