A5019067997- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Nerve injury and regeneration
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Immune cells in cancer
- Axon Guidance and Neuronal Signaling
- Cell Adhesion Molecules Research
- Calpain Protease Function and Regulation
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Planarian Biology and Electrostimulation
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Proteoglycans and glycosaminoglycans research
- Mesenchymal stem cell research
- Lipid Membrane Structure and Behavior
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- RNA Interference and Gene Delivery
- Apelin-related biomedical research
- Nuclear Receptors and Signaling
University of Edinburgh
2011-2024
Technical University of Munich
2015-2022
Munich Cluster for Systems Neurology
2017-2022
Ludwig-Maximilians-Universität München
2018-2019
Mott MacDonald (United Kingdom)
2017
Ruhr University Bochum
2009-2015
MRC Centre for Regenerative Medicine
2011-2014
Multiple Sclerosis Society
2013-2014
The majority of axons in the central nervous system (CNS) are eventually myelinated by oligodendrocytes, but whether timing and extent myelination vivo reflect intrinsic properties or regulated axons, remains undetermined. Here, we use zebrafish to study CNS at single-cell resolution vivo. We show that large caliber Mauthner axon is first be (shortly before smaller caliber) presence supernumerary can profoundly affect single oligodendrocytes. Oligodendrocytes typically myelinate just one...
The length of myelin sheaths affects conduction speed along axons and information propagation. It has recently become clear that may be adaptively modified to modulate circuit function, implying remodeling should occur. However, direct evidence for such events is lacking. We have investigated how myelination patterns are formed, maintained, remodeled using long-term imaging ablation in zebrafish. demonstrate differences between established by rapid variable growth within 3 days after their...
Abstract Many oligodendrocyte precursor cells (OPCs) do not differentiate to form myelin, suggesting additional roles of this cell population. The zebrafish optic tectum contains OPCs in regions devoid myelin. Elimination these impaired precise control retinal ganglion axon arbor size during formation and maturation retinotectal connectivity degraded functional processing visual stimuli. Therefore, fine-tune neural circuits independently their canonical role make
Abstract Membrane formation and the initiation of myelin gene expression are hallmarks differentiation oligodendrocytes from their precursors. Here, we compared roles two related extracellular matrix (ECM) glycoproteins Tenascin C (Tnc) R (Tnr) in oligodendrocyte differentiation. Oligodendrocyte precursors Tnr‐deficient mice exhibited reduced differentiation, as revealed by retarded basic protein (MBP) culture. This could be rescued with purified Tnr. In contrast, when cultured on a...
Here, we present mechanisms for the inhibition of oligodendendrocyte precursor cell (OPC) differentiation, a biological function neural extracellular matrix (ECM). The differentiation oligodendrocytes is orchestrated by complex set stimuli. In study, investigated signaling pathway elicited ECM glycoprotein tenascin C (Tnc). Tnc substrates inhibit myelin basic protein (MBP) expression cultured rat oligodendrocytes, and, conversely, found that emergence MBP accelerated in forebrains...
The correct targeting of myelin is essential for nervous system formation and function. Oligodendrocytes in the CNS myelinate some axons, but not others, do structures including cell bodies dendrites [1Lubetzki C. Demerens Anglade P. Villarroya H. Frankfurter A. Lee V.M. Zalc B. Even culture, oligodendrocytes solely axons.Proc. Natl. Acad. Sci. USA. 1993; 90: 6820-6824Crossref PubMed Scopus (111) Google Scholar]. Recent studies indicate that extrinsic signals, such as neuronal activity...
Chondroitin sulfate proteoglycans (CSPGs) have been proven to inhibit morphological maturation of oligodendrocytes as well their myelination capabilities. Yet, it remained unclear, whether CSPGs and/or respective chondroitin glycosaminoglycan (CS-GAG) side chains also regulate the oligodendrocyte lineage progression. Here, we initially show that CS-GAGs detected by monoclonal antibody 473HD are expressed primary rat NG2-positive precursor cells (OPCs) and O4-positive immature...
Abstract Protecting the nervous system from chronic effects of physical and chemical stress is a pressing clinical challenge. The obligate pro-degenerative protein Sarm1 essential for Wallerian axon degeneration. Thus, blocking function emerging as promising neuroprotective strategy with therapeutic relevance. Yet, conditions that will most benefit inhibiting remain undefined. Here we combine genome engineering, pharmacology high-resolution intravital videmicroscopy in zebrafish to show...
There is increasing evidence that the stem and progenitor cell population builds central nervous system very heterogeneous. Stem markers with potential to divide this pool into subpopulations distinct characteristics are sparse. We were looking for new type-specific antigens further subdivide pool. Here, we introduce novel monoclonal antibody clone 5750. show it specifically labels surfaces of neural cells. When 5750-expressing cells isolated by fluorescence-activated sorting from embryonic...
Demyelination is observed in numerous diseases of the central nervous system, including multiple sclerosis (MS). However, endogenous regenerative process remyelination can replace myelin lost disease, and various animal models. Unfortunately, often fails, particularly with ageing. Even when occurs, it characterised by regeneration sheaths that are abnormally thin short. This imperfect likely to have implications for restoration normal circuit function possibly optimal metabolic support...
The velocity of nerve conduction along vertebrate axons depends on their ensheathment with myelin. Myelin membranes comprise specialized proteins well characterized in mice. Much less is known about the protein composition myelin non-mammalian species. Here, we assess proteome biochemically purified from brains adult zebrafish ( Danio rerio ), considering its increasing popularity as model organism for biology. Combining gel-based and gel-free proteomic approaches, identified > 1,000...
The spacing of nodes Ranvier crucially affects conduction properties along myelinated axons. It is assumed that node position primarily driven by growing myelin sheaths. Here, we reveal an additional mechanism positioning the axon. Through longitudinal live imaging formation dynamics in zebrafish central nervous system, show stable clusters cell adhesion molecule neurofascin a can accumulate at specific sites axons prior to myelination. While some these are pushed into future extending...
The oligodendrocyte progenitors (OPCs) are at the front of glial reaction to traumatic brain injury. However, regulatory pathways steering OPC as well role reactive OPCs remain largely unknown. Here, we compared a long-lasting, exacerbated adult zebrafish injury with timely restricted activation identify specific molecular mechanisms regulating reactivity and their contribution regeneration. We demonstrated that influx cerebrospinal fluid into parenchyma after simultaneously activates...
Neuronal remodeling and myelination are two fundamental processes during neurodevelopment. How they influence each other remains largely unknown, even though their coordinated execution is critical for circuit function often disrupted in neuropsychiatric disorders. It unclear whether stabilizes axon branches or ongoing delays myelination. By modulating synaptic transmission, cytoskeletal dynamics, axonal transport mouse motor axons, we show that local onset node formation. Conversely, glial...