A5028891927- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Insect and Pesticide Research
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Helicobacter pylori-related gastroenterology studies
- Biosensors and Analytical Detection
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Bacillus and Francisella bacterial research
- Glycosylation and Glycoproteins Research
- Bacterial Identification and Susceptibility Testing
- Bee Products Chemical Analysis
- Streptococcal Infections and Treatments
- RNA Interference and Gene Delivery
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
University of Minho
2016-2025
University of Coimbra
2024-2025
Institute for Biotechnology and Bioengineering
2011-2013
Universidade Federal de São Paulo
2007
Phages are recognized as the most abundant and diverse entities on planet. Their diversity is determined predominantly by their dynamic adaptation capacities when confronted with different selective pressures in an endless cycle of coevolution a widespread group bacterial hosts. At end infection cycle, progeny virions rigid cell wall that hinders release into environment opportunity to start new cycle. Consequently, phages encode hydrolytic enzymes, called endolysins, digest peptidoglycan....
Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence a biofilm contributes to an increased tolerance of the antibiotics. Combined treatments bacteriophages antibiotics have shown promising antibiofilm activity, due profound differences their mechanisms action. In this study, 48 h old mono dual-species biofilms were treated with newly isolated P. infecting phage...
Proteus mirabilis is an enterobacterium that causes catheter-associated urinary tract infections (CAUTIs) due to its ability colonize and form crystalline biofilms on the catheters surface. CAUTIs are very difficult treat, since biofilm structures highly tolerant antibiotics. Phages have been used widely control a diversity of bacterial species, however limited number phages for P. isolated studied. Here we report isolation two novel virulent phages, podovirus vB_PmiP_5460 myovirus...
Chronic wound management is extremely challenging because of the persistence biofilm-forming pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, which are prevailing bacterial species that co-infect chronic wounds. Phage therapy has gained an increased interest to treat biofilm-associated infections, namely when combined with antibiotics. Here, we tested effect gentamicin a co-adjuvant phages in dual species-biofilm model formed on artificial dermis. The biofilm-killing...
Chronic wounds afford a hostile environment of damaged tissues that allow bacterial proliferation and further wound colonization.
Chronic skin wounds represent a major burn both economically and socially. Pseudomonas aeruginosa Escherichia coli are among the most common colonizers of infected prolific biofilm formers. Biofilms problem in infections due to their increasingly difficult control eradication, tolerance multiple prescribed drugs. As so, alternative methods necessary. Bacteriophages (phages) honey seen as promising approach for related infections. Phages have specificity toward bacterial genus, species or...
Staphylococcus epidermidis is considered an important nosocomial pathogen, being very tolerant to the host immune system and antibiotherapy, particularly when in biofilms. Due its high resistance, alternative antimicrobial strategies are under development. The use of bacteriophages seen as strategy combat pathogenic organisms. In this study, a S. myovirus, SEP1, was isolated characterized. genome phage sequenced shown be related peripherally genus Twortlikevirus. However, compared with other...
Bacteriophages are the most abundant and diverse entities in biosphere, this diversity is driven by constant predator–prey evolutionary dynamics horizontal gene transfer. Phage genome sequences under-sampled therefore present an untapped uncharacterized source of genetic diversity, typically characterized highly mosaic genomes no universal genes. To better understand relationships among phages infecting human pathogens, we have analysed complete 205 Staphylococcus sp. These predicted to...
Abstract Chronic wounds affect thousands of people worldwide, causing pain and discomfort to patients represent significant economical burdens health care systems. The treatment chronic is very difficult complex, particularly when are colonized by bacterial biofilms which highly tolerant antibiotics. Enterococcus faecium faecalis within the most frequent bacteria present in wounds. Bacteriophages (phages) have been proposed as an efficient alternative against antibiotic-resistant infections,...
Staphylococcus aureus is one of the most relevant opportunistic pathogens involved in many biofilm-associated diseases, and a major cause nosocomial infections, mainly due to increasing prevalence multidrug-resistant strains. Consequently, alternative methods eradicate pathogen are urgent. It has been previously shown that polyvalent staphylococcal kayviruses their derived endolysins excellent candidates for therapy. Here we present characterization new bacteriophage: vB_SauM-LM12 (LM12)....
Studies involving antimicrobial-coated endotracheal tubes are scarce, and new approaches to control multidrug-resistant Pseudomonas aeruginosa biofilm on these devices should be investigated. In this study, five P. bacteriophages from domestic sewage were isolated. All of them belong the order Caudovirales, Myoviridae family. They pH heat stable produce 27 46 particles after a latent period 30 min at 37oC. Their dsDNA genome (ranging ⁓62 kb ⁓65 kb), encode 65 89 different putative proteins....
Endolysins, which are peptidoglycan-degrading enzymes expressed during the terminal stage of reproduction cycle bacteriophages, have great potential to control Gram-positive pathogens. This work describes characterization a novel endolysin (PlyPl23) encoded on genome Paenibacillus larvae phage phiIBB_Pl23 with high American foulbrood. bacterial disease, caused by P. larvae, is widespread in North America and Europe causes important economic losses apiculture. The restriction antibiotic...
Staphylococcus epidermidis is a major causative agent of nosocomial infections, mainly associated with the use indwelling devices, on which this bacterium forms structures known as biofilms. Due to biofilms’ high tolerance antibiotics, virulent bacteriophages were previously tested novel therapeutic agents. However, several staphylococcal shown be inefficient against In study, characterized S. epidermidis-specific Sepunavirus phiIBB-SEP1 (SEP1), has broad spectrum and activity planktonic...
Abstract Bloodstream infections (BSIs) are considered a major cause of death worldwide. Staphylococcus spp. one the most BSIs prevalent bacteria, classified as high priority due to increasing multidrug resistant strains. Thus, fast, specific and sensitive method for detection these pathogens is extreme importance. In this study, we have designed novel assay in blood culture samples, which combines advantages phage endolysin cell wall binding domain (CBD) probe with accuracy high-throughput...