A5076330048- Polyomavirus and related diseases
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Full-Duplex Wireless Communications
- Antenna Design and Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Viral-associated cancers and disorders
- Parvovirus B19 Infection Studies
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Tumors and Oncological Cases
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Energy Harvesting in Wireless Networks
- Cancer Cells and Metastasis
- Animal Genetics and Reproduction
- Spaceflight effects on biology
- Glycosylation and Glycoproteins Research
Baylor College of Medicine
2009-2020
Baylor University
1964-2018
Dalhousie University
2010
Public Health Agency of Canada
2010
GlaxoSmithKline (United States)
2003
National Institute of Allergy and Infectious Diseases
2003
Deleted Institution
2003
Access to Wholistic and Productive Living Institute
2003
National Space Biomedical Research Institute
2002
Johns Hopkins University Center for AIDS Research
2001
Ependymomas and papillomas of the choroid plexus occur in early childhood. The ubiquitous human polyomaviruses, BK virus JC virus, have been associated with induction these neoplasms animal models. A related monkey polyomavirus, simian 40 (SV40), is highly tumorigenic rodents also induces papillomas.
An epithelial cell line, designated COMMA-1D, was derived from mammary tissue of BALB/c mice in the middle pregnancy. This continuous culture for 12 months, exhibits several characteristics distinctive normal cells, including induction casein synthesis vitro and duct morphogenesis cleared fat pads syngeneic mice. The cells also form domes high density are positive keratin intermediate filaments by indirect immunofluorescence. COMMA-1D have a near diploid number chromosomes do not grow...
Mouse, hamster, and human cells were transformed at the permissive temperature by mutants from simian virus 40 (SV40) complementation group A in order to ascertain role of gene function transformation. The following parameters transformation monitored with under nonpermissive conditions: morphology; saturation density; colony formation on plastic, cell monolayers, soft agar; uptake hexose; expression SV40 tumor (T) surface (S) antigens. Cells temperature-sensitive (ts) exhibited phenotype...
Simian virus 40 (SV40), a polyomavirus of rhesus macaque origin, was discovered in 1960 as contaminant polio vaccines that were distributed to millions people from 1955 through early 1963. SV40 is potent DNA tumor induces tumors rodents and transforms many types cells culture, including those human origin. This has been favored laboratory model for mechanistic studies molecular processes eukaryotic cellular transformation. The viral replication protein, named large T antigen (T-ag), also the...
The hepatitis B virus X protein (HBx) is a broadly acting transactivator implicated in the development of liver cancer. Recently, HBx has been reported to interact with several different cellular proteins, including our report its binding XAP-1, human homolog simian repair UVDDB. In present study, mutants were used localize minimal domain required for XAP-1/UVDDB amino acids 55 101. normal function thought involve damaged DNA, first step nucleotide excision (NER); therefore, we hypothesized...
The mechanism of action hepatitis B virus (HBV) X protein in transcriptional transactivation and tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular that can interact with HBV protein. This protein, designated X-associated 1 (XAP-1), is human homolog UV-damaged DNA-binding (UV-DDB) recovered from monkey cell cDNA library. UV-DDB presumed be involved DNA repair. interaction between XAP-1 was verified by immunoprecipitation lysates expressing both...
The hepatitis B virus X protein acts as a transcriptional transactivator in vitro. To elucidate possible biological effects of on liver cells vivo, we generated four lines transgenic mice carrying the gene open reading frame under control human alpha-1-antitrypsin regulatory region. plasmid construct used to introduce transgene was shown encode 16-kDa with transactivating capability. expression detectable tissue animals three by immunoblot analysis; levels were highest first month after...
Hamsters bearing tumors induced either by simian papovavirus 40 (SV40) or cells tranformed in vitro the virus develop antibodies capable of detecting a new complement-fixing antigen transformed cells.1 Antibodies also for intranuclear present all SV40 and detected immunofluorescence technique.2, 3 It appears that antigens complement-fixation are similar identical immunologically.4 The tumor is presumably under control at least portion genome, but some cell lines producing do not go on to...
Laser ablation MC-ICP-MS allows in situ strontium isotope data to be obtained for incrementally formed bioapatites such as enamel with extremely high spatial resolution. Here, we provide a large-scale application of the method comparing ...Understanding mobility and landscape use is important reconstructing subsistence behavior, range, group size, it may contribute our understanding phenomena dynamics biological cultural interactions between distinct ...
The effect of DNA antagonists and various antibiotics on steps in the synthesis SV40 virus green monkey kidney cells was investigated. Both early forming tumor (T) antigen, as well later synthesized (V) were presence fluorouracil iododeoxyuridine. Cytosine arabinoside (and fluorodeoxyuridine starved cells) prevented V antigen but not T antigen. therefore does require DNA. Virus particles formed only iododeoxyuridine they non-infectious. Actinomycin D inhibited both antigens, suggesting that...
SummaryHamsters bearing virus-free tumors induced by cells transformed in vitro or vivo papovavirus SV40 produce circulating antibody capable of reacting with intranuclear antigens synthesized SV40-transformed cells. The reaction is particulate and does not involve the nucleolus. All cultures, regardless whether they are hamster human origin, synthesize antigen.We wish to acknowledge able technical assistance supplied Myron Katz.
The role of the hepatitis B virus (HBV) X protein in liver tumorigenesis is unresolved. Transgenic mice harboring gene (nt 1376–1840 under control human α-1-antitrypsin regulatory elements) (ATX mice) display only minor histopathologic alterations liver. To determine if ATX are more susceptible to effects hepatocarcinogens, 12- 15-d-old male and littermate were injected with a single dose (2 μg/g body weight) diethylnitrosamine (DEN). animals killed 6–10 mo after exposure analyzed for...
Humans are infected with viruses that establish long-term persistent infections. To address whether immunocompetent individuals control virus reactivation globally or independently and to identify patterns of sporadic reactivation, we monitored herpesviruses polyomaviruses in 30 adults, over 14 months. Epstein-Barr (EBV) DNA was quantitated saliva peripheral blood mononuclear cells (PBMCs), cytomegalovirus (CMV) assayed urine, JC (JCV) BK (BKV) DNAs were urine PBMCs. All shed EBV saliva,...