A5059510199- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Bacterial Genetics and Biotechnology
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Protein Structure and Dynamics
- CRISPR and Genetic Engineering
- Streptococcal Infections and Treatments
- Lipid Membrane Structure and Behavior
- Artificial Intelligence in Healthcare
- Enterobacteriaceae and Cronobacter Research
- Genomics and Phylogenetic Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Artificial Intelligence in Healthcare and Education
- Healthcare cost, quality, practices
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
University of Sheffield
2019-2025
University of York
2024
Abstract Many bacteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface plays crucial roles in physiology. Here, we report crystal structure of SlpA, main S-layer bacterial pathogen Clostridioides difficile , use electron microscopy to study organisation assembly. The SlpA lattice mimics assembly cell, through tiling triangular prisms above wall, interlocked by distinct ridges facing environment. Strikingly, array is very compact, with pores only...
The molecular details of phage tail contraction and bacterial cell envelope penetration remain poorly understood are completely unknown for phages infecting bacteria enveloped by proteinaceous S-layers. Here, we reveal the extended contracted atomic structures an intact contractile-tailed (φCD508) that binds to penetrates protective S-layer Gram-positive human pathogen Clostridioides difficile . is unusually long (225 nm), it also notable contracts less than those studied in related...
Abstract The alpha helical CytolysinA family of pore forming toxins (α-PFT) contains single, two, and three component members. Structures the single Eschericia coli ClyA two Yersinia enterolytica YaxAB show both undergo conformational changes from soluble to forms, oligomerization produce active pore. Here we identify tripartite α-PFTs in pathogenic Gram negative bacteria, including Aeromonas hydrophila (AhlABC). We that AhlABC toxin requires all components for maximal cell lysis. present...
Abstract Tripartite members of the ClyA family α-PFTs have recently been identified in a number pathogenic Gram-negative bacteria, including human pathogen Serratia marcescens . Structures A component and tripartite α-PFT complete pore are unknown mechanism for formation is still uncertain. Here we characterise SmhABC toxin from S. propose assembly. We present structure soluble SmhA, as well forms SmhB. show that β-tongue conserved two latches between head tail domains broken on to...
Abstract Transducing bacteriophages and gene transfer agents (GTA) are constrained by their capsids’ structural properties in the length of host DNA they can package. Nanopore sequencing intact capsid-packaged molecules with full-sized reads be used to establish precise lengths identity individual packaged association specific bacterial hosts. This approach was validated using a few well-characterised transducing systems, then applied study encapsidation faecal microbiomes from three healthy...
Abstract Viruses that infect bacteria (bacteriophages or phages) attach to the host cell envelope, inject their genetic material into cytosol and either persist as prophage hijack machinery produce progeny virions. Attachment is mediated through phage receptor binding proteins are specific for different surface molecules. A subset of phage, myoviruses, possess contractile tails, outer sheath which contracts upon binding, driving an inner tail tube envelope delivering genome cytosol. The...
Abstract Many bacteria and archaea possess a cell surface layer – S-layer made of 2D protein array that covers the entire cell. As outermost component envelope, S-layers play crucial roles in many aspects physiology. Importantly, clinically relevant bacterial pathogens distinct forms an initial interface with host, making it potential target for development species-specific antimicrobials. Targeted therapeutics are particularly important antibiotic resistant such as Clostridioides difficile...
The Artificial Intelligence (AI) for Human-Robot Interaction (HRI) Symposium has been a successful venue of discussion and collaboration since 2014. During that time, these symposia provided fertile ground numerous collaborations pioneered many discussions revolving trust in HRI, XAI service robots, interactive learning, more. This year, we aim to review the achievements AI-HRI community last decade, identify challenges facing ahead, welcome new researchers who wish take part this growing...