A5092798220- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- RNA modifications and cancer
- Biochemical and Molecular Research
- Microbial Natural Products and Biosynthesis
- Metalloenzymes and iron-sulfur proteins
- Evolution and Genetic Dynamics
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- CRISPR and Genetic Engineering
The University of Texas at Dallas
2023-2025
Reactive oxygen species (ROS) generate DNA lesions that alter genome integrity. Among those lesions, 7,8‐dihydro‐8‐oxo‐2′‐deoxyguanosine (8‐oxodG) is particularly mutagenic. 8‐oxodG efficiently incorporates deoxycytidine monophosphate (dCMP) and deoxyadenosine (dAMP) via base pairing mediated by its anti syn conformations, respectively. In family‐A polymerases (DNAPs), the amino acids responsible for modulating dCMP or dAMP incorporation across are located in a determined structural...
ABSTRACT MUTYH is a clinically important DNA glycosylase that thwarts mutations by initiating base-excision repair at 8-oxoguanine (OG):A lesions. The roles for its [4Fe-4S] cofactor in remain enigmatic. Functional profiling of cancer-associated variants near the revealed most variations abrogate both retention and enzyme activity. Surprisingly, R241Q N238S retained metal center bound substrate tightly but were completely inactive. We solved crystal structure human to transition state mimic...
Abstract MUTYH is a clinically important DNA glycosylase that thwarts mutations by initiating base-excision repair at 8-oxoguanine (OG):A lesions. The roles for its [4Fe-4S] cofactor in remain enigmatic. Functional profiling of cancer-associated variants near the reveals most variations abrogate both retention and enzyme activity. Surprisingly, R241Q N238S retained metal cluster bound substrate tightly, but were completely inactive. We determine crystal structure human to transition state...
Polyketide synthases (PKSs) are multidomain enzymatic assembly lines that biosynthesize a wide selection of bioactive natural products from simple building blocks. In contrast to their cis-acyltransferase (AT) counterparts, trans-AT PKSs rely on stand-alone ATs load extender units onto acyl carrier protein (ACP) domains embedded in the core PKS machinery. Trans-AT gene clusters also encode hydrolases (AHs), which predicted share overall fold but function like type II thioesterases (TEIIs),...
Enzymes are critical biological catalysts involved in maintaining the intricate balance of metabolic processes within living organisms. Mutations enzymes can result disruptions to their functionality, that may lead a range diseases. This review focuses on computational studies investigate effects disease-associated mutations various enzymes. Through molecular dynamics simulations, multiscale calculations, and machine learning approaches, provide detailed insights into how impact enzyme...
Polyketide synthases (PKSs) are multi-domain enzymatic assembly lines that biosynthesise a wide selection of bioactive natural products from simple building blocks. In contrast to their cis -acyltransferase (AT) counterparts, trans -AT PKSs rely on stand-alone AT domains load extender units onto acyl carrier protein (ACP) embedded in the core PKS machinery. Trans gene clusters also encode hydrolase (AH) domains, which predicted share overall fold but hydrolyse aberrant chains ACP thus...