A5027226572- Gut microbiota and health
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Genomics, phytochemicals, and oxidative stress
- Tryptophan and brain disorders
- Antimicrobial Peptides and Activities
- Pediatric health and respiratory diseases
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- MicroRNA in disease regulation
- Diet and metabolism studies
- Cannabis and Cannabinoid Research
- Immune Cell Function and Interaction
- Microscopic Colitis
- Probiotics and Fermented Foods
- Inflammatory Bowel Disease
- Dietary Effects on Health
- Stress Responses and Cortisol
- Anesthesia and Neurotoxicity Research
- Ginseng Biological Effects and Applications
- Regulation of Appetite and Obesity
- Adipokines, Inflammation, and Metabolic Diseases
- Epigenetics and DNA Methylation
- Helicobacter pylori-related gastroenterology studies
- Cancer, Stress, Anesthesia, and Immune Response
University of South Carolina
2015-2025
Abstract Inflammatory diseases of the gastrointestinal tract are often associated with microbial dysbiosis. Thus, dietary interactions intestinal microbiota, to maintain homeostasis, play a crucial role in regulation clinical disorders such as colitis. In current study, we investigated if resveratrol, polyphenol found variety foods and beverages, would reverse dysbiosis induced during Administration resveratrol attenuated colonic inflammation symptoms murine model...
Colitis, an inflammatory bowel disease, is caused by a variety of factors, but luminal microbiota are thought to play crucial roles in disease development and progression. Indole produced gut believed protect the colon from damage. In current study, we investigated whether indole-3-carbinol (I3C), naturally occurring plant product found numerous cruciferous vegetables, can prevent colitis-associated microbial dysbiosis attempted identify mechanisms. Treatment with I3C led repressed colonic...
Abstract The compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an environmental contaminant, is a potent ligand for aryl hydrocarbon receptor (AhR). In the current study, we made exciting observation that naive C57BL/6 mice were exposed i.p. to TCDD showed massive mobilization of myeloid-derived suppressor cells (MDSCs) in peritoneal cavity. These MDSCs highly immunosuppressive and attenuated Con A–induced hepatitis upon adoptive transfer. administration also led induction several...
Alpha-defensin 1 is a small antimicrobial peptide that acts as the first line of defense against pathogens. It induced following microbial cues and inflammatory signals in neutrophils Paneth cells intestine, which suggests it plays role homeostasis gut. The gut products also serve ligands for aryl hydrocarbon receptor (AhR), an environmental sensor. In current study, we investigated if there any crosstalk between AhR alpha-defensin 1. Interestingly, found positive correlation protein levels...
Inflammatory bowel disease (IBD) is known to significantly increase the risk for development of colorectal cancer (CRC), suggesting inflammation and are closely intertwined. Thus, agents that suppress may prevent onset cancer. In current study, we used resveratrol, an anti-inflammatory stilbenoid, study role microbiota in preventing inflammation-driven CRC. Resveratrol treatment azoxymethane (AOM) dextran sodium sulphate (DSS) CRC murine model caused CD4 + FOXP3 (Tregs) IL10 cells, a...
Staphylococcal enterotoxin B (SEB) is a potent superantigen produced by Staphylococcus aureus that triggers strong immune response, characterized cytokine storm, multi-organ failure, and often death. When inhaled, SEB can cause acute lung injury (ALI) respiratory failure. In this study, we investigated the effect of resveratrol (RES), phytoallexin, on SEB-driven ALI mortality in mice. We used dual-exposure model C3H/HeJ mice, which caused 100% within first 5 days exposure, treatment with RES...
Scope Colitis, an inflammatory bowel disease, is associated with aberrant regulation of the colonic mucosal immune system. Resveratrol, a natural plant product, has been found to exert anti‐inflammatory properties and attenuate development murine colitis. In current study, role microRNA (miR) in ability resveratrol suppress inflammation examined. Methods results BALB/C mice 2,4,6‐Trinitrobenzenesulfonic acid solution (TNBS)‐induced colitis, when treated resveratrol, show improved clinical...
With increased climate change pressures likely to influence harmful algal blooms, exposure microcystin, a known hepatotoxin and byproduct of cyanobacterial blooms can be risk factor for NAFLD associated comorbidities. Using both in vivo vitro experiments we show that microcystin mice cause rapid alteration gut microbiome, rise bacterial genus mediating inflammation lactate production. Changes the microbiome were strongly with inflammatory pathology intestine, leaching, tight junction protein...
We previously reported that an aryl hydrocarbon receptor (AhR) ligand, indole-3-carbinol (I3C), was effective at reducing colitis severity through immune cell-mediated interleukin-22 (IL-22) production. Intestinal epithelial cells (IECs) are also involved in regulating colitis, so we investigated their AhR-mediated mechanisms the current report. A transcriptome analysis of IECs wildtype (WT) mice revealed during I3C regulated select mucin proteins, which could be attributed to goblet cell...
Staphylococcal enterotoxin B (SEB) is a potent superantigen capable of inducing inflammation characterized by robust immune cell activation and proinflammatory cytokine release. Exposure to SEB can result in food poisoning as well fatal conditions such toxic shock syndrome. In the current study, we investigated effect natural indoles including indole-3-carbinol (I3C) 3,3'-diindolylmethane (DIM) on SEB-mediated liver injury. Injection into D-galactosamine-sensitized female C57BL/6 mice...
Dysbiosis in gut microbiome has been shown to be associated with inflammatory and autoimmune diseases. Previous studies from our laboratory demonstrated the pivotal role played by CD44 regulation of EAE, a murine model multiple sclerosis. In current study, we determined whether these effects resulted an alteration microbiota short‐chain fatty acid (SCFA) production knockout (CD44KO) mice. Fecal transfer naïve CD44KO but not C57BL/6 wild type (CD44WT) mice, into EAE‐induced CD44WT led...
Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) potent ligand for AhR known carcinogen. While activation by TCDD leads to significant immunosuppression, how this translates into carcinogenic signal unclear. Recently, we demonstrated that of in naïve C57BL6 mice massive induction myeloid derived-suppressor cells (MDSCs). In the current study, investigated role gut microbiota TCDD-mediated MDSC induction. caused...
Acute respiratory distress syndrome (ARDS) is a serious lung condition characterized by severe hypoxemia leading to limitations of oxygen needed for function. In this study, we investigated the effect anandamide (AEA), an endogenous cannabinoid, on Staphylococcal enterotoxin B (SEB)-mediated ARDS in female mice. Single-cell RNA sequencing data showed that epithelial cells from AEA-treated mice increased levels antimicrobial peptides (AMPs) and tight junction proteins. MiSeq 16S LEfSe...
The Aryl Hydrocarbon Receptor (AhR) is a transcription factor that, when activated by ligand-binding, has been shown to regulate the immune response. Pertussis Toxin (PTX) virulence found in Bordetella pertussis, human respiratory pathogen that causes whooping cough. PTX promotes colonization and disease promotion triggering heightened inflammatory role of AhR regulation PTX-mediated inflammation not previously studied. In current study, we investigate if activation...
Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that regulates T cell function. The aim of this study was to investigate the effects AhR ligands, 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), and 6-Formylindolo[3,2-b]carbazole (FICZ), on gut-associated microbiota responses during delayed-type hypersensitivity (DTH) reaction induced by methylated bovine serum albumin (mBSA) in mouse model. Mice with DTH showed significant changes gut including an increased abundance...
Colitis is an inflammatory bowel disease (IBD) characterized by immune cell dysregulation and alterations in the gut microbiome. In our previous report, we showed a natural product cruciferous vegetables ligand of aryl hydrocarbon receptor (AhR), indole-3-carbinol (I3C), was able to reduce colitis-induced severity microbial dysbiosis interleukin-22 (IL-22) dependent manner.
Abstract Background Posttraumatic stress disorder (PTSD) is a psychiatric accompanied by chronic peripheral inflammation. What triggers inflammation in PTSD currently unclear. In the present study, we identified potential defects signaling pathways blood mononuclear cells (PBMCs) from individuals with PTSD. Methods RNAseq (5 samples each for controls and PTSD), ChIPseq each) miRNA array (6 were used combination bioinformatics tools to identify dysregulated genes PBMCs. Real time qRT-PCR (24...
Post-traumatic stress disorder (PTSD) is a psychiatric and patients diagnosed with PTSD often express other comorbid health issues, particularly autoimmune inflammatory disorders. Our previous reports investigating peripheral blood mononuclear cells (PBMCs) from showed that these exhibit an increased T helper (Th) cell phenotype widespread downregulation of microRNAs (miRNAs), key molecules involved in post-transcriptional gene regulation. A combination analyzing prior datasets on miRNA...
Cannabinoid receptor [CB] activation can attenuate inflammatory bowel disease [IBD] in experimental models and human cohorts. However, the roles of microbiome, metabolome, respective contributions haematopoietic non-haematopoietic cells anti-colitic effects cannabinoids have yet to be determined.Female C57BL/6 mice were treated with either cannabidiol [CBD], Δ 9-tetrahydrocannabinol [THC], a combination CBD THC, or vehicle, several chemically induced colitis. Clinical parameters colitis...