A5055687012- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Total Knee Arthroplasty Outcomes
- Various Chemistry Research Topics
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Pesticide Residue Analysis and Safety
- Retinal Development and Disorders
- Photosynthetic Processes and Mechanisms
- Hemoglobin structure and function
- Shoulder Injury and Treatment
- Cancer Research and Treatments
- DNA and Nucleic Acid Chemistry
- Porphyrin Metabolism and Disorders
- Knee injuries and reconstruction techniques
- Orthopaedic implants and arthroplasty
- Orthopedic Surgery and Rehabilitation
- Protein Structure and Dynamics
- Hormonal and reproductive studies
- Pharmacological Effects and Assays
- Advanced biosensing and bioanalysis techniques
University of Manchester
2013-2024
AstraZeneca (United Kingdom)
2022-2023
The initial stages of protein unfolding may reflect the stability entire fold and can also reveal which parts a be perturbed, without restructuring rest. In this work, we couple UVPD with activated ion mobility mass spectrometry to measure how three model proteins start unfold. Ubiquitin, cytochrome c myoglobin ions produced via nESI from salty solutions are subjected UV irradiation pre-mobility separation; experiments conducted range source conditions alter conformation precursor as shown...
Introduction The infrapatellar branch of the saphenous nerve is often injured during total knee arthroplasty (TKA), leading to numbness in its distribution distal incision. This illustrated a patient who suffered full-thickness burns lateral aspect from hot water bottle. However, proportion patients are informed this phenomenon (as well as effect informing numbness) has not previously been studied according authors’ knowledge. aim study was establish with whom postoperative discussed consent...
The infrapatellar branch of the saphenous nerve is often injured during total knee arthroplasty (TKA), leading to numbness in its distribution distal incision. This illustrated a patient who suffered full-thickness burns lateral aspect from hot water bottle. However, proportion patients are informed this phenomenon (as well as effect informing numbness) has not previously been studied according authors' knowledge. aim study was establish with whom postoperative discussed consent procedure...
The function of the bacterial photoreceptor protein, CarH, is regulated by changes to its oligomeric state. Camacho <italic>et al.</italic> detail how binding vitamin B<sub>12</sub> in dark drives assembly protein tetramer that turn blocks transcription.
A lab-based experiment, combining ion mobility mass spectrometry (IM-MS) with computational modelling techniques for the analysis of small molecules, has been developed use in undergraduate teaching laboratories over eight years. Students link IM-MS calculations to show how their interplay can elucidate structures molecules chosen by students. This experiment offers an opportunity gain experience a wide range skills including experimental design, sample preparation, instrument tuning,...
Activated ion mobility measurements provide Insights to the stability of tertiary and quaternary structures proteins pairing such approaches with fragmentation can delineate which part(s) primary sequence are disrupted from a folded structure. In this work we use 213 nm photodissociation coupled mass spectrometry collisional activation determine conformational landscape model proteins. UVPD experiments performed on following in source as well collisionally activated photoproducts post...
In this work we couple UVPD with activated ion mobility mass spectrometry to measure how three model proteins start unfold. Ubiquitin, cytochrome c and myoglobin ions produced via nESI from salty solutions are subjected UV irradiation pre-mobility separation, experiments conducted a range of source conditions which alter the conformation precursor as shown by drift time profiles. For all compact structures result in less fragmentation than more extended emerge following progressive in-source...
In this work we couple UVPD with activated ion mobility mass spectrometry to measure how three model proteins start unfold. Ubiquitin, cytochrome c and myoglobin ions produced via nESI from salty solutions are subjected UV irradiation pre-mobility separation, experiments conducted a range of source conditions which alter the conformation precursor as shown by drift time profiles. For all compact structures result in less fragmentation than more extended emerge following progressive in-source...
<p>Activated ion mobility measurements provide Insights to the stability of tertiary and quaternary structures proteins pairing such approaches with fragmentation can delineate which part(s) primary sequence are disrupted from a folded structure. In this work we use 213 nm photodissociation coupled mass spectrometry collisional activation determine conformational landscape model proteins. UVPD experiments performed on following in source as well collisionally activated photoproducts...