A5071232562- Congenital gastrointestinal and neural anomalies
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Digestive system and related health
- Wnt/β-catenin signaling in development and cancer
- Intestinal Malrotation and Obstruction Disorders
- DNA Repair Mechanisms
- Kruppel-like factors research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Cystic Fibrosis Research Advances
- Gastrointestinal motility and disorders
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
Royal Netherlands Academy of Arts and Sciences
2013-2017
Cancer Genomics Centre
2017
Hubrecht Institute for Developmental Biology and Stem Cell Research
2013-2017
University Medical Center Utrecht
2013-2017
The Francis Crick Institute
2013
The University of Texas Medical Branch at Galveston
2012
A signature event for organoids Human cancer genomes harbor cryptic mutational signatures that represent the cumulative effects of DNA damage and defects in repair processes. Knowledge how specific originate could have a major impact on diagnosis prevention. One approach to address this question is reproduce experimental systems by genetic engineering then match those found naturally occurring cancers. Drost et al. used CRISPR-Cas9 delete certain enzymes from human colon organoids. In...
A highly complex network of intrinsic enteric neurons is required for the digestive and homeostatic functions gut. Nevertheless, genetic molecular mechanisms that regulate their assembly into functional neuronal circuits are currently unknown. Here we report planar cell polarity (PCP) genes Celsr3 Fzd3 during murine embryogenesis to specifically control guidance growth projections relative longitudinal radial gut axes. Ablation these disrupts normal organization nascent projections, leading...
Abstract In this unit, we describe a protocol for regulated gene expression in primary endodermal organoid culture using retroviral vectors. The study of function epithelia such as those lining the stomach, small intestine, and colon has so far mainly relied on generation transgenic mouse lines. Establishing animal models is laborious, expensive, time‐consuming. Ever‐expanding organoids, grown an vitro 3‐D epithelial system, faithfully recapitulate vivo counterpart represent sustainable...
Stem cell-based therapy has recently been explored for the treatment of disorders enteric nervous system (ENS). Pluripotent embryonic stem (ES) cells represent an attractive cell source; however, little or no information is currently available on how ES will respond to gut environment. In this study, we investigated ability environmental cues derived from ENS and related tissues, both in vitro vivo.Neurospheres were generated mouse (ES-NS) co-cultured with organotypic preparations tissue...