A5030036328- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacterial biofilms and quorum sensing
- Neuroscience and Neuropharmacology Research
- Parasitic Infections and Diagnostics
- Receptor Mechanisms and Signaling
- Infectious Diseases and Mycology
- Plant tissue culture and regeneration
- Amoebic Infections and Treatments
- Plant Pathogens and Fungal Diseases
- Antimicrobial Resistance in Staphylococcus
- Hormonal and reproductive studies
- Parasites and Host Interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Antibiotics Pharmacokinetics and Efficacy
- Neutropenia and Cancer Infections
- Infective Endocarditis Diagnosis and Management
- Legionella and Acanthamoeba research
- Nail Diseases and Treatments
- Ion channel regulation and function
- Viral gastroenteritis research and epidemiology
- Mycotoxins in Agriculture and Food
- Patient Satisfaction in Healthcare
- Vector-borne infectious diseases
- Wheat and Barley Genetics and Pathology
Oklahoma Medical Research Foundation
2024
Dartmouth–Hitchcock Medical Center
2019-2023
University of Glasgow
2004-2020
Portsmouth Hospitals NHS Trust
2020
Glasgow Royal Infirmary
2009-2018
NHS Greater Glasgow and Clyde
2011-2018
Oregon Health & Science University
2014-2016
Portland VA Medical Center
2014
Stobhill Hospital
2013
The University of Texas at San Antonio
2009
Aspergillus fumigatus is often isolated from the lungs of cystic fibrosis (CF) patients, but unlike in severely immunocompromised individuals, mortality rates are low. This suggests that competition bacteria within CF lung may be inhibitory. The purpose this study was to investigate how Pseudomonas aeruginosa influences A. conidial germination and biofilm formation. formation inhibited by direct contact with P. aeruginosa, had no effect on preformed biofilm. A secreted heat-stable soluble...
A 12 month survey of candidaemia in Scotland, UK, which every Scottish hospital laboratory submitted all blood isolates yeasts for identification, strain typing and susceptibility testing, provided 300 from 242 patients, generating incidence data 4.8 cases per 100 000 population year 5.9 acute occupied bed days; 27.9 % occurred intensive care units. More than half the patients with had an underlying disease involving abdomen, 78 indwelling intravenous catheter, 62 suffered a bacterial...
ABSTRACT This study investigated the phase-dependent expression and activity of efflux pumps in Aspergillus fumigatus treated with voriconazole. Fourteen strains were shown to become increasingly resistant 12-h (16- 128-fold) 24-h (>512-fold) phases compared 8-h germlings. An Ala-Nap uptake assay demonstrated a significant increase pump ( P < 0.0001). The germling cells was also significantly induced by voriconazole 0.001) after 24 h treatment. Inhibition competitive substrate...
Candida albicans infections have become increasingly recognised as being biofilm related. Recent studies shown that there is a relationship between formation and poor clinical outcomes in patients infected with proficient strains. Here we investigated panel of isolates an attempt to evaluate their phenotypic transcriptional properties differentiate define levels formation.Biofilm was be heterogeneous; defined either high or low formers (LBF HBF) based on different biomass quantification....
Aspergillus fumigatus undergoes morphological transition throughout its growth and development. These changes have direct implications for the effectiveness of antifungal treatment. Here we report in vitro activity voriconazole, amphotericin B caspofungin against three specific phases multicellular development A. fumigatus. conidia were propagated 8, 12 24 h prior to challenge. The resultant agents tested was determined using an XTT reduction assay assess both endpoint time–kill...
Vulvovaginal candidiasis (VVC) is a global health problem affecting ∼75% of women at least once in their lifetime. Here we examined the epidemiology VVC patient cohort to identify causative organisms associated with VVC. Biofilm-forming capacity and antifungal sensitivity profiles were also assessed. We report shifting prevalence Candida species heterogeneous biofilm-forming capacity, which altered drug sensitivity.
This study provide an up-to-date overview of the epidemiology and risk factors for Candida bloodstream infection in Scotland 2012/2013, antifungal susceptibility isolates from blood cultures 11 National Health Service boards within Scotland. were identified by chromogenic agar confirmed MALDI-TOF methods. Survival associated patients stratified as albicans non-albicans cases assessed. Information on spectrum antifungals used was collected summarised. The sensitivity to different tested broth...
Biofilms formed by Candida albicans bloodstream isolates on catheters are an important clinical problem. Devising chemotherapeutic strategies to treat these in situ is attractive option. We report here that liposomal amphotericin effectively kills C. biofilms rapidly (12 h) and (>90%) a dose-dependent manner, whereas caspofungin displays inverse concentration-dependent effect. This study has implications for considering the effective doses of antifungal agents used catheter lock therapy.
Abstract Candida albicans biofilm formation is an important virulence factor in the pathogenesis of disease, a characteristic which has been shown to be heterogeneous clinical isolates. Using unbiased computational approach we investigated central metabolic pathways driving heterogeneity. Transcripts from high (HBF) and low (LBF) forming isolates were analysed by RNA sequencing, with 6312 genes identified expressed these two phenotypes. With dedicated validated significantly differentially...
The purpose of this study was to investigate the performance non-invasive diagnostic tests such as galactomannan enzyme immunoassay and quantitative PCR in early diagnosis invasive aspergillosis (IA), how these are impacted upon by use different classes antifungal agents an in-vivo model IA.A standardised rat inhalation IA used examine effects azole, posaconazole, a polyene, amphotericin B echinocandin caspofungin. Daily blood samples were collected for subsequent analysis using commercially...
Carbohydrate derived fulvic acid (CHD-FA) is a heat stable low molecular weight, water soluble, cationic, colloidal material with proposed therapeutic properties. The aim of this study was to evaluate the antifungal activity CHD-FA against Candida albicans, and characterise its mode action. A panel C. albicans isolates (n=50) from range clinical specimens were grown planktonically as biofilms, minimum inhibitory concentrations (MICs) determined. Scanning electron microscopy performed examine...
This is the first report to characterize genotypes and subtypes of Cryptosporidium species infecting a geographically isolated population feral Soay sheep (Ovis aries) on Hirta, St. Kilda, Scotland, during two distinct periods: (i) prior crash (ii) as host numbers increased. DNA was extracted by freeze-thawing immunomagnetically separated (IMS) bead-oocyst complexes, were identified following nested-PCR-restriction fragment length polymorphism (RFLP)/PCR sequencing at 18S rRNA loci. Two...