A5030004637- Traumatic Brain Injury and Neurovascular Disturbances
- Metabolomics and Mass Spectrometry Studies
- S100 Proteins and Annexins
- Mitochondrial Function and Pathology
- Asthma and respiratory diseases
- Metabolism and Genetic Disorders
- Heart Rate Variability and Autonomic Control
- Traumatic Brain Injury Research
- Diet and metabolism studies
- Ion Channels and Receptors
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Respiratory and Cough-Related Research
- Eicosanoids and Hypertension Pharmacology
- Muscle metabolism and nutrition
- Pulmonary Hypertension Research and Treatments
- Immunotherapy and Immune Responses
- Multiple Sclerosis Research Studies
- Neurological Disease Mechanisms and Treatments
- Gastrointestinal motility and disorders
- Animal testing and alternatives
- Fatty Acid Research and Health
- Neurobiology and Insect Physiology Research
- Immunotoxicology and immune responses
- Biochemical Acid Research Studies
- Probiotics and Fermented Foods
University of South Florida
2022-2024
Roskamp Institute
2019-2023
James A. Haley Veterans' Hospital
2019-2022
The Open University
2022
Abstract Background Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex illness which disproportionally affects females. This associated with immune and metabolic perturbations that may be influenced by lipid metabolism. We therefore hypothesized plasma lipids from ME/CFS patients will provide unique biomarker signature of disturbances in immune, inflammation processes ME/CFS. Methods Lipidomic analyses were performed on cohort 50 controls (50% males similar age...
Gulf War Illness (GWI), affecting 30% of veterans from the 1991 (GW), is a multi-symptom illness with features similar to those patients autoimmune diseases. The objective current work determine if exposure GW-related pesticides, such as permethrin (PER), activates peripheral and central nervous system (CNS) adaptive immune responses. In study, we focused on PER metabolite, 3-phenoxybenzoic acid (3-PBA), this common metabolite previously shown form adducts endogenous proteins. We observed...
The differential diagnosis between mild Traumatic Brain Injury (mTBI) sequelae and Post-Traumatic Stress Disorder (PTSD) is challenging due to their symptomatic overlap co-morbidity. As such, there a need develop biomarkers which can help with of these two conditions. Studies from our group others suggest that blood brain lipids are chronically altered in both mTBI PTSD. Therefore, examining presents minimally invasive cost-effective approach identify promising Using liquid...
Transient receptor potential ankyrin 1 (TRPA1) is a polymodal cation channel that activated by electrophilic irritants, oxidative stress, cold temperature, and GPCR signaling. TRPA1 expression has been primarily identified in subsets of nociceptive sensory afferents considered target for future analgesics. Nevertheless, implicated other cell types including keratinocytes, epithelium, enterochromaffin cells, endothelium, astrocytes, CNS neurons. Here, we developed knock-in mouse expresses the...
Most vagal sensory afferents innervating the lower airways are activated by noxious stimuli including irritants (e.g. TRPV1 agonist capsaicin) and inflammatory mediators, causing nociceptive cardiorespiratory reflexes cough, bronchospasm, changes in respiratory drive heart rate). Vagal ganglia comprised of embryologically distinct nodose jugular neurons, but little is known their specific contribution to reflexes. Using a novel TRPV1Flp mouse combination with P2X2Cre, Tac1Cre, intersectional...
Currently approved blood biomarkers detect intracranial lesions in adult patients with mild to moderate traumatic brain injury (TBI) acutely post-injury. However, are still needed help a differential diagnosis of TBI (mTBI) and post-traumatic stress disorder (PTSD) at chronic post-injury time points. Owing the association between phospholipid (PL) dysfunction consequences TBI, we hypothesized that examining bioactive PL metabolites (oxylipins ethanolamides) would identify long-term lipid...
With age the apolipoprotein E ( APOE ) E4 allele (involved in lipid homeostasis) is associated with perturbation of bioenergetics pathways Alzheimer’s disease (AD). We therefore hypothesized that aging mice genotype would affect L-carnitine system (central to bioenergetics), brain and periphery. Using liquid chromatography-mass spectrometry, levels metabolites: γ-butyrobetaine (GBB), crotonobetaine, as well acylcarnitines, were evaluated at 10-, 25-, 50-weeks, periphery, a targeted...
Diet and commercially available supplements can significantly impact the gut microbial composition; however, effects of often lack scientific data demonstrating on healthy diseased individuals. Hence, it was investigated, whether a frequently used supplement in humans, Candida rugosa lipase (CRL), gets delivered active beyond stomach intestinal tract C57BL/6 J mice its community environment. We showed for first time movement CRL an state through mouse digestive by determination activity free...
Abstract Repetitive mild traumatic brain injury (r-mTBI) is the most widespread type of trauma worldwide. The cumulative effect triggers long-lasting pathological and molecular changes that may increase risk chronic neurodegenerative diseases. R-mTBI also characterized by in proteome, where majority molecules altered early post-TBI are different from those at more phases. This differentiation contribute to heterogeneity available data on potential therapeutic targets present an obstacle...
Activation of airway sensory nerves causes respiratory and autonomic reflexes. The majority the afferents are only sensitive to noxious stimuli, such as inflammation, irritants, pollutants. these nociceptive evokes protective mechanisms apnea, cough, bradycardia. These reflexes may contribute disease morbidity when excessively activated. Airway nociceptors, largely projecting from vagal ganglia (nodose jugular ganglion), heterogeneous with respect gene expression neuroanatomy, our objective...
Abstract Repetitive mild traumatic brain injury (r-mTBI) is the most widespread type of traumas worldwide. The cumulative effect triggers long-lasting pathological and molecular changes that may increase a risk chronic neurodegenerative diseases. R-mTBI also characterized by in proteome, where majority molecules altered early post-TBI are different from those at more phases. This differentiation contribute to heterogeneity available data on potential therapeutic targets present an obstacle...
Activation of airway sensory nerves causes respiratory and autonomic reflexes. The majority the are only sensitive to noxious stimuli, such as inflammation, infection, irritants pollutants. these nociceptive evokes protective mechanisms apnea, cough bradycardia. However, reflexes may also contribute disease morbidity when excessively or inappropriately activated. Airway nerves, which largely projected from vagal ganglia (nodose jugular ganglion), heterogeneous with respect gene expression...
Activation of airway sensory nerves causes respiratory and autonomic reflexes. The majority the are only sensitive to noxious stimuli, such as inflammation, infection, irritants pollutants. these nociceptive evokes protective mechanisms apnea, cough bradycardia that can contribute disease morbidity. Airway heterogeneous with respect gene expression neuroanatomy, our objective is characterize specific reflexes evoked by activation afferent subsets. To selectively activate vagal subpopulations...
Background: The apolipoprotein E (APOE) ε4 allele, involved in fatty acid (FA) metabolism, is a major genetic risk factor for Alzheimer’s disease (AD). This study examined the influence of APOE genotypes on blood and brain markers L-carnitine system, necessary oxidation (FAO), their collective clinical pathological outcomes AD.Methods: L-carnitine, its metabolites γ-butyrobetaine (GBB) trimethylamine-n-oxide (TMAO), esters (acylcarnitines) were analyzed from community/clinic-based...