A5017349373- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Signaling Pathways in Disease
- CAR-T cell therapy research
- Viral gastroenteritis research and epidemiology
- HIV/AIDS drug development and treatment
- Monoclonal and Polyclonal Antibodies Research
Eli Lilly (United States)
2022
La Jolla Institute for Immunology
2015-2016
International AIDS Vaccine Initiative
2015
Natural infections expose the immune system to escalating antigen and inflammation over days weeks, whereas nonlive vaccines are single bolus events. We explored whether responds optimally kinetics most similar replicating infections, rather than a dose. Using HIV antigens, we found that administering given total dose of adjuvant 1-2 wk through repeated injections or osmotic pumps enhanced humoral responses, with exponentially increasing (exp-inc) dosing profiles eliciting >10-fold increases...
ABSTRACT Generating neutralizing antibodies (nAbs) is a major goal of many current HIV-1 vaccine efforts. To be practical value, these nAbs must both potent and cross-reactive in order to capable preventing the transmission highly diverse generally neutralization resistant (Tier-2) strains that are circulation. The envelope glycoprotein (Env) spike only target for nAbs. explore whether Tier-2 can induced by Env proteins, we immunized conventional mice with soluble BG505 SOSIP.664 trimers...
Abstract Follicular CD4+ Th (Tfh) cells provide B cell help in germinal center reactions that support class switching, somatic hypermutation, and the generation of high-affinity Abs. In this article, we show deficiency NFAT1 NFAT2 T leads to impaired upon viral infection because reduced Tfh differentiation defective expression proteins involved T/B interactions help, including ICOS, PD-1, SLAM family receptors. Genome-wide chromatin immunoprecipitation data suggest NFAT likely directly...
Abstract The pathobiology of rheumatoid inflammatory diseases, including arthritis (RA) and psoriatic arthritis, involves the interplay between innate adaptive immune components resident synoviocytes. Single-cell analyses patient samples relevant mouse models have characterized many cellular subsets in RA. However, impact interactions cell types is not fully understood. In this study, we temporally profiled murine arthritic synovial isolates at single-cell level to identify perturbations...
SAP (SH2D1A) is required intrinsically in CD4 T cells to generate germinal center responses and long-term humoral immunity. binds SLAM family receptors, including SLAM, CD84, Ly108 enhance cytokine secretion sustained cell:B cell adhesion, which both improve follicular helper (Tfh) aid (GC) B cells. To understand the overlapping roles of multiple receptors responses, Slamf1Δ/Δ Slamf5Δ/Δ Slamf6Δ/Δ triple gene disruption (Slamf1,5,6Δ/Δ) mice were generated using CRISPR-Cas9 editing eliminate...
Abstract Follicular helper T (Tfh) cells provide B cell help in germinal center reactions that support class switching, somatic hypermutation, and the generation of high-affinity antibodies. Here we show deficiency NFAT1 NFAT2 CD4+ leads to impaired upon viral infection due reduced Tfh differentiation defective expression proteins involved T:B interactions help, including ICOS, PD-1, SLAM family receptors. ChIP-seq data suggest NFAT likely directly participate regulation genes important for...