A5050453074- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Insect-Plant Interactions and Control
- Plant and Biological Electrophysiology Studies
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Cocoa and Sweet Potato Agronomy
- Plant Parasitism and Resistance
- Advances in Cucurbitaceae Research
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Micronutrient Interactions and Effects
- Plant Genetic and Mutation Studies
Agricultural Research Organization
2009-2022
The objective of the project was to study salinity‐induced effects on essential oil, pigments and salts accumulation in sweet basil ( Ocimum basilicum , cultivar Perrie) relation alteration plant morphological development yield production. Hydroponically grown plants were exposed one six NaCl concentrations (1, 25, 50, 75, 100 130 mM NaCl). Inhibitory salinity biomass production shoot root, area individual leaves apparent already under cultivation with 25 NaCl. Elevation from 1 induced 63%...
Salinity inhibits growth and development of most plants. The response to salinity is complex varies between plant organs stages development. It involves challenges ion toxicities deficiencies as well osmotic oxidative stresses. range functions affected by the stress reflected in elaborate changes transcriptome. mechanisms involved developmental-stage specificity inhibitory responses are not fully understood. present study took advantage characterized developmental progression that exists...
Deeply conserved plant microRNAs (miRNAs) function as pivotal regulators of development. Nevertheless, in the model crop Solanum lycopersicum (tomato) several miRNAs are still poorly annotated and knowledge about their functions is lacking. Here, tomato miR171 family was functionally analyzed. We found that genome contains at least 11 SlMIR171 genes differentially expressed along Downregulation sly-miR171 successfully achieved by transgenic expression a short tandem target mimic construct...
RNA-dependent RNA polymerase 1 (RDR1) plays a crucial role in plant defence against viruses. In this study, it was observed that cucumber, Cucumis sativus, uniquely encodes small gene family of four RDR1 genes. The cucumber genes (CsRDR1a, CsRDR1b and duplicated CsRDR1c1/c2) shared 55%-60% homology their encoded amino acid sequences. healthy plants, RDR1a RDR1b transcripts were expressed at higher levels than RDR1c1/c2, which barely detectable. expression all CsRDR1 induced by virus...
Abstract During tobamovirus–host coevolution, tobamoviruses developed numerous interactions with host susceptibility factors and exploited these for replication movement. The plant‐encoded TOBAMOVIRUS MULTIPLICATION (TOM) proteins interact the tobamovirus replicase allow formation of viral complex. Here CRISPR/Cas9‐mediated mutagenesis allowed exploration roles SlTOM1a , SlTOM1b SlTOM3 in systemic infection tomato. Knockouts both sltom1a/sltom3 plants resulted an asymptomatic response to...
DICER-like 1 (DCL1) is a major player in microRNA (miRNA) biogenesis and accordingly, its few known loss-of-function mutants are either lethal or display arrested development. Consequently, generation of dcl1 by reverse genetics functional analysis DCL1 late-developing organs challenging. Here, these challenges were resolved through the unique use trans-activated RNA interference. Global, as well organ-specific tomato (SlDCL1) silencing was induced crossing generated responder line...
The HAIRY MERISTEM (HAM) genes function in meristem maintenance but play minor roles the morphogenesis of a simple leaf that is determinate. Here, we functionally analyzed HAM tomato and uncovered their involvement compound morphogenesis. Tomato encodes three homologs, which SlHAM SlHAM2 (SlHAMs) are guided for cleavage by microRNA171 abundant shoot floral meristems as well primordia. We found SlHAMs silencing led to overproliferation cells periphery where localized. As meristems,...
Powdery mildew (PM) diseases may severely limit the production of various crops, including members family Cucurbitaceae. Successful PM infection relies on Mildew Resistance Locus O (MLO) plant gene family, which encodes susceptibility factors essential for fungus penetration into host cell. In cucumber (Cucumis sativus), natural mutations in CsaMLO8 confer resistance to pathogen Podosphaera xanthii. Here, we used CRISPR/Cas9-mediated mutagenesis generate susceptible cultivar Ilan. Two...