Single-stranded DNA constrained on a graphene surface is effectively protected from enzymatic cleavage by DNase I. Various spectroscopy studies suggest that the single-stranded promptly adsorbed onto forming strong molecular interactions. Constraint of probe improves specificity its response to complementary DNA. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted...

Abstract Carbon nanodots (C‐dots) show great potential as an important material for biochemical sensing, energy conversion, photocatalysis, and optoelectronics because of their water solubility, chemical inertness, low toxicity, photo‐ electronic properties. Numerous methods have been proposed the preparation C‐dots. However, complex procedures strong acid treatments are often required, as‐prepared C‐dots tend to be quality, in particular, a efficiency photoluminescence. Herein, facile...

High quality carbon dots (C-dots) with down- and up-conversion fluorescence have been synthesized through low-temperature carbonization using sweet pepper as the source. The C-dots a quantum yield (QY) of 19.3% exhibit superior photophysical properties, for example, narrow symmetric emission spectra, large stock shifts, resistance to photobleaching, excitation-dependent behavior. excellent serve useful fluorescent probes hypochlorite (ClO−) detection by both fluorescence. Two consecutive...

Exosomes, as novel noninvasive biomarkers for disease prediction and diagnosis, have shown fascinating prospects in monitoring cancer-linked public health issues. Herein, a unique Cy3 labeled CD63 aptamer (Cy3-CD63 aptamer)/Ti3C2 MXenes nanocomplex was constructed self-standard ratiometric fluorescence resonance energy transfer (FRET) nanoprobe quantitative detection of exosomes. The Cy3-CD63 can be selectively adsorbed onto the Ti3C2 MXene nanosheets by hydrogen bond metal chelate...

Biological thiols play a critical role in biological processes and are involved variety of diseases. The discrimination detection is increasing importance clinical diagnosis. In this paper, novel nanosensor was developed to discriminate cysteine (Cys) from homocysteine (Hcy) glutathione (GSH) with multiple signals: colorimetric, photoluminescence (PL), up-conversional (UCP). (NC-dots/AuNPs) constructed by nitrogen-doped carbon dots (NC-dots) gold nanoparticles (AuNPs) through assembling...

In this paper, a new strategy for the construction of multifunctional electrochemical detection platforms based on Michael addition/Schiff base reaction polydopamine modified reduced graphene oxide was first proposed. Inspired by mussel adhesion proteins, 3,4-dihydroxyphenylalanine (DA) selected as reducing agent to simultaneously reduce and self-polymerize obtain polydopamine-reduced (PDA-rGO). The PDA-rGO then functionalized with thiols amines thiol/amino groups quinine via reaction....

Abstract Studying numerous biologically important species simultaneously is crucial to understanding cellular functions and the root causes of related diseases. Direct visualization endogenous biothiols in biological systems great value their roles. Herein, a novel multi‐signal fluorescent probe was rationally designed exploited for simultaneous sensing homocysteine (Hcy), cysteine (Cys), glutathione (GSH) using different emission channels. This successfully applied discrimination between...

Three-dimensional graphite carbon nitride (3D-CN) has received tremendous research interest due to its unique structural features, fascinating physicochemical properties, and widespread potential applications. Most syntheses of 3D-CN mainly focus on developing g-C3N4 (2.7 eV) using soft-templating hard-templating strategies. Nevertheless, the excellent photocatalytic activity is related small band gap, which relevant structure nitrogen (N) content. Meanwhile, it still a challenge prepare...

Alzheimer's disease (AD) and Parkinson's (PD) are chronic neurodegenerative diseases with high morbidity mortality. Homocysteine (Hcy), cysteine (Cys), glutathione (GSH) closely related to AD PD. However, the dynamics of Hcy, Cys, GSH in brain tissues potential pathogenesis between Cys/Hcy/GSH PD remain unclear. Herein, a novel fluorescent probe 1 multiple binding sites was rationally designed exploited for direct quantification serum total Hcy Cys along superior optical properties....

An electrochemiluminescence (ECL) sensor provides a sensitive and convenient method for early diagnosis of diseases; however, it is still challenge to develop simple sensing platforms based on efficient ECL signals luminophore groups. Porphyrin-based metal-organic frameworks (MOFs) show great potential in sensing; the mechanism structure-activity relationship, as well application, are rarely reported. Herein, hydrothermal reactions obtained porphyrin Zr-MOFs (PCN-222) with different specific...

Here, a sensitive and selective strategy for the detection of nitrite via colorimetry test strips was developed based on Fe-single-atom catalysts (Fe SACs) through oxidation–reduction diazotization reactions. Fe SACs possess excellent oxidase-like (OXD) catalytic activity, which can catalyze oxidation colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue TMBox with appearance an obvious absorbance peak at 652 nm in presence oxygen. With addition (NO2–), reactions between TMB/TMBox induce...

Cuproptosis is a new type of copper-induced cell death that characterized by the aggregation lipoylated tricarboxylic acid (TCA) cycle proteins. However, its role in hepatocellular carcinoma (HCC) remains unclear. The goal this research to develop cuproptosis-related signature predicting prognosis HCC.The genes were defined using Pearson correlation coefficients. LASSO-Cox regression analysis was used evaluate prognostic values construct model. immune microenvironment performed "ssGSEA"...

Cerebral ischemia–reperfusion injury (CIRI), a cause of cerebral dysfunction during infarction treatment, is closely associated with mitochondrial viscosity and hydrogen peroxide (H2O2). However, the accurate measurement H2O2 levels in CIRI challenging because lack sufficient selectivity blood–brain barrier (BBB) penetration existing monitoring tools related to CIRI, hampering exploration role CIRI. To address this issue, we designed an activatable fluorescent probe, mitochondria-targeting...

Cytokines are essential components of the immune system and recognized as significant biomarkers. However, detection a single cytokine is not precise reliable enough to satisfy requirements for diagnosis. Herein, we developed pattern recognition-based method multiplexed sensing cytokines, which involves three-color-emitting boronic acid-decorated carbon dots (BCDs) arginine-modified titanium carbide (Ti3C2 MXenes) sensor array. Initially, fluorescence signals three BCDs were quenched by...

Thermoresponsive microcontainers (see Figure) based on crosslinked poly(N-isopropylacrylamide) shells undergo a reversible swelling transition upon changing the temperature of system. This has considerable influence permeability these microcontainers, which makes systems highly attractive for encapsulation and release biomolecules such as enzymes, proteins, or DNA.

Sensitive, rapid, and simple detection methods for the screening of extensively used organophosphorus pesticides highly toxic nerve agents are in urgent demand. A novel label-free silicon quantum dots (SiQDs)-based sensor was designed ultrasensitive pesticides. This sensing strategy involves reaction acetylcholine chloride (ACh) with acetylcholinesterase (AChE) to form choline that is turn catalytically oxidized by oxidase (ChOx) produce betaine H2O2 which can quench photoluminescence (PL)...

We demonstrate a multivalent recognition and highly selective aptamer signal amplification strategy for electrochemical cytosensing dynamic cell surface N-glycan expression evaluation by the combination of concanavalin A (Con A), mannose binding protein, as model, conjugated poly(amidoamine) dendrimer on chemically reduced graphene oxide (rGO–DEN) interface, aptamer- horseradish peroxidase-modified gold nanoparticles (HRP–aptamer–AuNPs) nanoprobes. In this strategy, rGO–DEN can not only...