A5057912428- Salivary Gland Disorders and Functions
- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Ocular Surface and Contact Lens
- Fibroblast Growth Factor Research
- Corneal Surgery and Treatments
- Axon Guidance and Neuronal Signaling
- Mesenchymal stem cell research
- Pancreatic function and diabetes
- dental development and anomalies
- Neonatal Respiratory Health Research
- Wnt/β-catenin signaling in development and cancer
- Connective tissue disorders research
- Nerve injury and regeneration
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Cleft Lip and Palate Research
- Craniofacial Disorders and Treatments
- Corneal surgery and disorders
- Cancer, Hypoxia, and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
- Osteoarthritis Treatment and Mechanisms
- Nasolacrimal Duct Obstruction Treatments
- Advanced biosensing and bioanalysis techniques
University of California, San Francisco
2015-2025
National Institutes of Health
2007-2015
Morpho (United States)
2011-2015
National Institute of Dental and Craniofacial Research
2007-2015
UNSW Sydney
2001-2008
University of Minnesota
2005-2007
University of Alberta
2005
Icahn School of Medicine at Mount Sinai
2002-2003
The University of Texas Southwestern Medical Center
2003
Commonwealth Scientific and Industrial Research Organisation
2001-2002
The maintenance of a progenitor cell population as reservoir undifferentiated cells is required for organ development and regeneration. However, the mechanisms by which epithelial are maintained during organogenesis poorly understood. We report that removal parasympathetic ganglion in mouse explant culture decreased number morphogenesis keratin 5-positive cells. These effects were rescued with an acetylcholine analog. demonstrate signaling, via muscarinic M1 receptor epidermal growth factor...
Organogenesis requires the complex interactions of multiple cell lineages that coordinate their expansion, differentiation, and maturation over time. Here, we profile types within epithelial mesenchymal compartments murine pancreas across developmental time using a combination single-cell RNA sequencing, immunofluorescence, in situ hybridization, genetic lineage tracing. We identify previously underappreciated cellular heterogeneity developing mesenchyme reconstruct potential relationships...
Heparan sulfate proteoglycans are essential for biological processes regulated by fibroblast growth factors (FGFs). (HS) regulates the activity of FGFs acting as a coreceptor at cell surface, enhancing FGF-FGFR affinity, and being storage reservoir in extracellular matrix (ECM). Here we demonstrate critical role heparanase during mouse submandibular gland (SMG) branching morphogenesis. Heparanase, an endoglycosidase, colocalized with perlecan basement membrane epithelial clefts SMGs....
Research Article15 January 2018Open Access Transparent process Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement Elaine Emmerson orcid.org/0000-0002-5902-3368 Program in Craniofacial Biology, Department of Cell and Tissue University California, San Francisco, CA, USA Search for more papers by this author Alison J May Lionel Berthoin Noel Cruz-Pacheco Sara Nathan Aaron Mattingly Jolie L Chang Otolaryngology, William R Ryan D Tward...
Acinar cells play an essential role in the secretory function of exocrine organs. Despite this requirement, how acinar are generated during organogenesis is unclear. Using acini-ductal network developing human and murine salivary gland, we demonstrate unexpected for SOX2 parasympathetic nerves generating lineage that has broad implications epithelial morphogenesis. being expressed by progenitors give rise to both duct cells, genetic ablation results a failure establish acini but not ducts....
Salivary proteins are essential for maintaining health in the oral cavity and proximal digestive tract, they serve as potential diagnostic markers monitoring human disease. However, their precise organ origins remain unclear. Through transcriptomic analysis of major adult fetal salivary glands integration with saliva proteome, blood plasma transcriptomes 28+ organs, we link to source, identify salivary-gland-specific genes, uncover fetal- adult-specific gene repertoires. Our results also...
Human basement membrane heparan sulfate proteoglycan (HSPG) perlecan binds and activates fibroblast growth factor (FGF)-2 through its (HS) chains. Here we show that perlecans immunopurified from three cellular sources possess different HS structures subsequently FGF-2 binding activating capabilities. Perlecan isolated human umbilical arterial endothelial cells (HUAEC) a continuous cell line (C11 STH) bound similar amounts of either alone or complexed with FGFRα1-IIIc FGFR3α-IIIc. Both...
Perlecan is a large multidomain proteoglycan that essential for normal cartilage development. In this study, perlecan was localized in the pericellular matrix of hypertrophic chondrocytes developing human rudiments. immunopurified from medium conditioned by cultured fetal found to be substituted with heparan sulfate (HS), chondroitin (CS), and keratan (KS). Ligand carbohydrate engagement (LACE) assays demonstrated chondrocyte-derived formed HS-dependent ternary complexes fibroblast growth...
The aim of this study was to immunolocalize perlecan in human fetal, postnatal, and mature hyaline cartilages determine information on the structure function chondrocyte perlecan. Perlecan is a prominent component fetal (12-14 week) finger, toe, knee, elbow cartilages; it localized diffusely interterritorial extracellular matrix, densely pericellular matrix around chondrocytes, small blood vessels joint capsules perichondrium. Aggrecan had more intense distribution marginal regions rudiments...
The tear producing lacrimal gland is a tubular organ that protects and lubricates the ocular surface. While possesses many features make it an excellent model to understand tubulogenesis, cell types lineage relationships drive formation are unclear. Using single sequencing other molecular tools, we reveal novel identities epithelial dynamics underlie development. We show from its earliest developmental stages composed of multiple subpopulations immune, epithelial, mesenchymal lineages....
ABSTRACT The critical cellular transitions that govern human pancreas development are largely unknown. We performed large-scale single-cell RNA-sequencing (scRNA-Seq) to interrogate fetal from 8-20 weeks post conception. identified 103 distinct cell types, including four novel endocrine progenitor subtypes displaying unique transcriptional features and differentiation potency. Integration with single-nucleus Assay for Transposase Accessible Chromatin Sequencing (snATAC-Seq) candidate...
How novel gene functions evolve is a fundamental question in biology. Mucin proteins, functionally but not evolutionarily defined group of allow the study convergent evolution function. By analyzing genomic variation mucins across wide range mammalian genomes, we propose that exonic repeats and their copy number contribute substantially to de novo new functions. integrating bioinformatic, phylogenetic, proteomic, immunohistochemical approaches, identified 15 undescribed instances...
The lacrimal gland (LG) secretes aqueous tears. Previous studies have provided insights into the cell lineage relationships during tissue morphogenesis. However, little is known about types composing adult LG and their progenitors. Using scRNAseq, we established first comprehensive atlas of mouse to investigate hierarchy, its secretory repertoire, sex differences. Our analysis uncovered complexity stromal landscape. Epithelium subclustering revealed myoepithelial cells, acinar subsets, two...
Determination and differentiation of cells in the skeletal muscle lineage is positively regulated by cell–cell contact. Cell-surface proteins proposed to mediate this effect include both classical cadherins Ig superfamily members; potential interactions between promyogenic activities these classes protein, however, are unknown. We show here that CDO BOC, two members bind each other a cis fashion, form complexes with N- M-cadherin. These contain β-catenin enriched at sites contact myoblasts....