- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Plant Pathogens and Resistance
- Nematode management and characterization studies
- Plant Pathogenic Bacteria Studies
- Allelopathy and phytotoxic interactions
- Transgenic Plants and Applications
- Forest Insect Ecology and Management
- Molecular Biology Techniques and Applications
- Fungal Plant Pathogen Control
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Management Techniques
- Insect symbiosis and bacterial influences
- Agriculture, Plant Science, Crop Management
- Parasite Biology and Host Interactions
- Microbial Metabolites in Food Biotechnology
- Potato Plant Research
- Pesticide Exposure and Toxicity
- Irrigation Practices and Water Management
- Research in Cotton Cultivation
- Genetically Modified Organisms Research
Institute of Plant Protection
2013-2024
ORCID
2023
Weatherford College
2021
Institute of Plant Protection
2012-2020
MRC Laboratory of Molecular Biology
2008-2014
Potato mop‐top virus (PMTV; genus Pomovirus ; family Virgaviridae ) is transmitted by the soil‐borne Spongospora subterranea f.sp. , a protoctist that causes powdery scab on potato. PMTV distributed widely in potato growing areas South and North America, Japan northwestern Europe. This article reviews current knowledge detection, distribution control of with focus Baltic Sea region. Since 1980s, has caused great economic losses to production Nordic countries (Norway, Sweden, Denmark...
Nowadays, plant protection against viral diseases is one of the most challenging tasks faced by modern agriculture. One possible ways utilization systemic acquired resistance phenomenon. In this approach biological or chemical factors interact with plants and stimulate their immune system infections before infection occurs. This paper presents 11 ionic derivatives benzo[1.2.3]thiadiazole-7-carboxy-S-methyl ester, a synthetic inducer, counterions specifically selected to modify physical...
Three isolates of Tomato torrado virus (ToTV) were found in Poland. The characterized on the basis their symptomatology plant species, serological reactions, electron microscopy, and nucleotide amino acid sequence analyses coat protein subunit genes. In comparative tests, Polish ToTV shown to be closely related each other also isolate from Spain.
Plant resistance induction is one of the most promising ways to support plants in fights against pathogens, especially viruses, due fact there are no plant protection agents acting directly on them. Certain chemicals, including benzo[1.2.3]thiadiazole-7-carbothioic acid, S-methyl ester (BTH) and its derivatives, were discovered as effective inducers immunity. In this article, new BTH form organic salts composed cations based inducer anions introduced order modify physical (solubility water,...
Positive-sense single-stranded [ss(+)] plant RNA viruses are obligate intracellular parasites that infect many agriculturally important plants, leading to severe losses of crops. All known characterized by small genomes encoding a limited number multifunctional viral proteins. Viral pathogens considered be absolutely dependent on host. To facilitate genomic replication, must recruit numerous host proteins and other factors. The replication process depends the virus – protein-protein,...
The first biologically active infectious clones of tomato torrado virus (ToTV) were generated and delivered into Nicotiana benthamiana Solanum lycopersicum plants via Agrobacterium tumefaciens. engineered constructs consisted PCR-amplified complementary DNAs derived from the ToTV RNA1 RNA2 components, individually inserted an pGreen binary vector between CaMV 35S promoter nopaline synthase terminator. These introduced plant hosts by means A. tumefaciens-mediated infiltration. In presence...
Peanut stunt virus (PSV), which belongs to the Cucumovirus genus, is a pathogen of legumes. Certain PSV strains associated with satellite RNA (satRNA) modify symptoms infected plants and interfere plant metabolism. We used PSV-P genomic transcripts (GTs) without satRNA comparative proteomic 2D-DIGE/MS study assess their effects on Nicotiana benthamiana infection. When proteomes transcripts-infected (no present) mock-inoculated were compared 29 differentially regulated proteins found....
Green fluorescent protein (GFP)-tagged viruses are basic research tools widely applied in studies concerning molecular determinants of disease during virus infection. Here, we described a new generation genetically stable infectious clones tomato torrado isolate Kra (ToTVpJL-Kra) that could infect Nicotiana benthamiana and Solanum lycopersicum. Importantly, modified variant the viral RNA2-with inserted sGFP (forming, together with RNA1, into ToTVpJL-KraGFP)-was engineered as well. RNA2...
Abstract The genus D itylenchus contains more than 80 recognized nematode species with a very wide host range. most serious are dipsaci and destructor . Populations of complex were collected from A llium cepa , C ichorium endivia P hlox paniculata in oland. gigas population was V icia faba minor populations S olanum tuberosum spp. Analyses the r DNA sequences spanning both ITS 1 2 fragment regions carried out on populations. obtained compared those deposited G en B ank isolated other...
Potato mop-top virus (PMTV) is a serious pathogen occurring in Northern Europe, North and South America, Asia that significantly reduces potato (Solanum tuberosum) production. PMTV transmitted by Spongospora subterranea, the casual agent of powdery scab, causes characteristic brown arcs circles (spraing symptoms) tubers, stunting stems, shortening internodes, mosaic patterns (V-shaped) on leaves as well leaf necrosis (2). S. subterranea are mainly associated with cool, humid environments....
Peanut stunt virus (PSV) is a common legume pathogen present worldwide. It also infectious for many other plants including peanut and some vegetables. Viruses of this species are classified at into three subgroups based on their serology nucleotide homology. Some them may carry an additional subviral element - satellite RNA. Analysis the full genome sequence Polish strain PSV-P associated with satRNA was performed showed that it be as derivative subgroup I sharing 83.9-87.9% homology members...
Abstract The genome sequences of two Polish Kra and Ros isolates Tomato torrado virus (To TV ) were determined compared with data previously described To other Torradovirus members. Whole‐genome sequence comparisons revealed 97.0–99.6% nucleotide identities close relatedness, known isolates. high homology between Kra, Wal'03 s is likely responsible for the similar symptoms observed on infected plants. However, differed in intensity various host specificity. We report that caused a milder...
Colorado potato beetle (CPB) (Leptinotarsa decemlineata (Say in Journal of the Academy Natural Sciences Philadelphia 3: 298–331, 1824)) (Coleoptera: Chrysomelidae) is one most serious pests. It has been reported worldwide, from North America to Europe and Asia. In this study we analyzed genetic diversity a mitochondrial DNA marker – second subunit cytochrome oxidase (mtCOII) Polish CPB populations assess possible changes gene sequence over time country, influencing intra-specific variability...
Abstract Globodera rostochiensis is one of the most important plant parasitic nematodes, worldwide. As a quarantine pest solanaceous crops, species often subjected to morphological and genetic analysis as well biological tests. They constitute basis for this nematode detection control. This paper presents results study on variability 16 populations golden potato cyst from Poland – producers using molecular techniques. The cluster based sequencing rDNA fragment showed slight differences among...
Bradysia species, commonly known as fungus gnats, are ubiquitous in greenhouses, nurseries of horticultural plants, and commercial mushroom houses, causing significant economic losses. Moreover, the insects from genus have a well-documented role plant pathogenic fungi transmission. Here, study on potential impatiens to acquire transmit peanut stunt virus (PSV) was undertaken. Four-day-old larvae B. were exposed PSV-P strain by feeding virus-infected leaves Nicotiana benthamiana then...
Abstract Tomato torrado virus (ToTV), a member of the Torradovirus genus, primarily infects tomatoes. Previous analyses revealed high heterogeneity in 3′ untranslated region (3′ UTR) RNA1 isolate ToTV‐Kra. In addition to full‐length UTR (a wild‐type, wt), four truncated versions (var2, var3, var4 and var5) were identified. Here, we investigated biological importance this phenomenon by assessing whether such defective genomes are infectious individually, how length influences disease...