A5058015770- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Pluripotent Stem Cells Research
- Systemic Lupus Erythematosus Research
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Sperm and Testicular Function
- Microtubule and mitosis dynamics
- Assisted Reproductive Technology and Twin Pregnancy
- Renal and related cancers
- Reproductive Health and Technologies
- Nuclear Structure and Function
- Dupuytren's Contracture and Treatments
- Retinoids in leukemia and cellular processes
- Animal Genetics and Reproduction
- Tendon Structure and Treatment
- Demographic Trends and Gender Preferences
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Inflammatory mediators and NSAID effects
- Bone health and treatments
- Ovarian function and disorders
- Skin and Cellular Biology Research
- Endometriosis Research and Treatment
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
Medical University of Sofia
2003-2024
BACKGROUND: Meiotic abnormalities are thought to be a major causal factor of low embryo development rates, for embryos developed from in vitro-matured oocytes. A new non-human primate model, the common marmoset, is being facilitate investigation mechanisms involved. METHODS: Oocytes were dissected antral follicles three size classes. They allowed mature vitro only 24 h, order focus on rapidly maturing Chromosome spreads visualized with Giemsa staining, and spindles /chromosomes fluorescently...
Three types of defects preimplantation embryogenesis contribute to the developmental arrest cleaving human embryos: blastomere fragmentation, abnormal nuclear status and chromosomal disorders. Data concerning relation succession these abnormalities during first mitotic cycles zygote are controversial mainly empirical at present. In this study we have performed simultaneous evaluation apoptotic changes ploidy four chromosomes (1, 5, 19 X or 18, 21, Y) in 193 embryos. Another group 28 embryos...
This study was aimed at elucidating the fate of three important nuclear envelope components - lamins B and A/C nucleoporin Nup160, during meiotic maturation mouse oocytes. These proteins were localized by epifluorescence confocal microscopy using specific antibodies in oocytes different stages from prophase I (germinal vesicle) to metaphase II. In immature germinal vesicle oocytes, all detected periphery. II, lamin co-localized with spindle, found a diffuse halo surrounding spindle lesser...
Summary The tubulin cytoskeleton is vital for maintenance and dynamics of eukaryotic cells molecular defects in its components can lead to serious conditions. So far, mutations genes alpha-, beta- gamma-tubulin, motor proteins the kinesin dynein family, microtubule-associated centrosomal have been found cause disorders humans. Most phenotypic effects are on nervous system, leading abnormal brain development (e.g. lissencephaly microcephaly) or neurodegeneration later life amyotrophic lateral...
Although a number of studies have shown that the occurrence and progression osteoarthritis (OA) is related to endocrine system dysfunction, there limited evidence about what roles sex hormones play. The aim present study was examine capacity 17β-estradiol (ED) follicle stimulating hormone (FSH) alter differentiation bone marrow (BM) cells in arthritic mice. experiments were conducted collagenase-induced Cartilage degradation observed by safranin toluidine blue staining. Flow cytometry used...
The effects of prostaglandin F2α on the cytoskeleton and membrane organelles oocytes was investigated by culturing ovulated mouse in its presence (50 or 100 ng/ml) for 3 h. Tubulin, fibrillar actin, membranes chromatin were visualized specific antibodies, phalloidin, lipophilic dye DiOC6 Hoechst 33342, respectively. Control characterized a meiotic spindle with chromosomes aligned at equator, cortical layer microfilaments an actin cap. Intracellular localized mostly central region metaphase I...
We studied apoptotic fragmentation in ovulated mouse oocytes.Some cells were fixed immediately after isolation, while others cultured for 3 hours with or without prostaglandin F2 alpha.Membrane organelles, fibrillar actin and DNA stained DiOC6, TRITC-phalloidin Hoechst 33342, respectively.While fragmented generally rare, most of them samples treated F2α, revealing it as a potential inducer apoptosis.The chromatin had interphase appearance, indicating exit from meiosis arrest at germinal...
Known as a degenerative joint disorder of advanced age affecting predominantly females, osteoarthritis can develop in younger and actively working people because activities involving loading injuries joints. Collagenase-induced (CIOA) mouse model allowed us to investigate for the first time its effects on key cytoskeletal structures (meiotic spindles actin distribution) ovulated oocytes. Their meiotic spindles, caps, chromatin were analyzed by immunofluorescence. A total 193 oocytes from...
Chromatin Quality as a Crucial Factor for the Success of Fluorescent in Situ Hybridization Analyses Unfertilized Oocytes, Polar Bodies and Arrested Zygotes Material that is supernumerary or unsuitable vitro fertilization (IVF) procedures used basic IVF-related research. Despite disadvantages such cells, they have contributed much to our understanding mechanisms prevalence different abnormalities. Fifty-four human unfertilized oocytes, 34 arrested bipronuclear zygotes 15 polar bodies were...
Summary The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – soluble, cytoskeletal NM-IF. results showed that some patients, but not control sera, react either the soluble fraction or NM-IF fraction. proteins most likely directed against surface...
In eukaryotic cells during interphase, the centrosome is positioned close to nucleus by a yet unidentified mechanism and determines basic cell polarity. The aim of this study was trace dynamics centriolar satellite protein PCM-1 in newborn mouse ovaria. Ovarian sections mice were subjected immunofluorescent analysis. intracellular localization prophase I oocytes compared between 1- 2-day-old mice. from 1-day-old ovaria, double or broader single discrete aggregates with perinuclear observed...
Investigation of the effect collagenase-induced osteoarthritis (CIOA) on mouse oocyte diameter and meiotic spindle defects showed significantly larger size higher proportion in CIOA oocytes compared to control group: detached fibers or fully disorganized spindles microtubule asters at poles were combined with a oocytes.