A5100754255- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Plant Stress Responses and Tolerance
- Plant Physiology and Cultivation Studies
- Studies on Chitinases and Chitosanases
- Plant tissue culture and regeneration
- Peptidase Inhibition and Analysis
- Plant Molecular Biology Research
- Enzyme Production and Characterization
- Plant responses to water stress
- interferon and immune responses
- Weed Control and Herbicide Applications
- Protein Hydrolysis and Bioactive Peptides
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
Howard Hughes Medical Institute
2019-2023
Brigham and Women's Hospital
2019-2023
Harvard University
2019-2023
Hunan University of Humanities, Science and Technology
2022
China Agricultural University
2008
The N-terminal residue influences protein stability through N-degron pathways. We used profiling of the human N-terminome to uncover multiple additional features In addition uncovering extended specificities UBR E3 ligases, we characterized two related Cullin-RING ligase complexes, Cul2ZYG11B and Cul2ZER1, that act redundantly target glycine. glycine degrons are depleted at native N-termini but strongly enriched caspase cleavage sites, suggesting roles for substrate adaptors ZYG11B ZER1 in...
The ubiquitin-proteasome system plays a critical role in biology by regulating protein degradation. Despite their importance, precise recognition specificity is known for few of the 600 E3s. Here, we establish two-pronged strategy identifying and mapping residues internal degrons on proteome-scale HEK-293T cells. We employ global stability profiling combined with machine learning to identify 15,800 peptides likely contain sequence-dependent degrons. combine this scanning mutagenesis define...
Chitooligosaccharides (COS), a high-value chitosan derivative, have many applications in food, pharmaceuticals, cosmetics and agriculture owing to their unique biological activities. Chitosanase, which catalyzes the hydrolysis of chitosan, can cleave β-1,4 linkages produce COS. In this study, chitosanase-producing Bacillus paramycoides BP-N07 was isolated from marine mud samples. The chitosanase enzyme (BpCSN) activity 2648.66 ± 20.45 U/mL at 52 h able effectively degrade chitosan. molecular...
The ubiquitin-proteasome system (UPS) is a complex regulatory network involving around 600 E3 ligases that collectively govern the stability of human proteome by targeting thousands proteins for degradation. Understanding this requires integrating vast amounts information on gene and protein interactions scattered across unstructured literature. Historically, manual curation has been gold standard transforming such data into structured databases, but process time-consuming, prone to error,...