A5103017236- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Analog and Mixed-Signal Circuit Design
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Phytochemicals and Antioxidant Activities
- Plant Virus Research Studies
- Biofuel production and bioconversion
- Plant pathogens and resistance mechanisms
- Insect Resistance and Genetics
- Bacteriophages and microbial interactions
- Crop Yield and Soil Fertility
- Plant Micronutrient Interactions and Effects
- Genetics and Plant Breeding
- Transgenic Plants and Applications
- Analytical Chemistry and Sensors
- GABA and Rice Research
- CRISPR and Genetic Engineering
Beijing Academy of Agricultural and Forestry Sciences
2015-2025
Peking University
2023-2025
Northwest A&F University
2021-2023
Zhongshan Hospital
2019-2022
Biotechnology Research Center
2004-2020
Yangtze University
2015-2019
Fudan University
2019
Sun Yat-sen University
2019
The First Affiliated Hospital, Sun Yat-sen University
2019
Biocon (Switzerland)
2019
Summary Plant roots respond to environmental stresses or the exogenous plant hormone abscisic acid (ABA) by undergoing marked physiological and morphological changes. We show here that PERK4 , a gene encodes member of Arabidopsis thaliana proline‐rich extensin‐like receptor kinase family, plays an important role in ABA responses. Mutation T‐DNA insertion decreased sensitivity with respect seed germination, seedling growth primary root tip growth. The effect on was due enhanced cell...
Current gene delivery methods for maize are limited to specific genotypes and depend on time-consuming labor-intensive tissue culture techniques. Here, we report a new method transfect that is culture-free genotype independent. To enhance efficiency of DNA entry maintain high pollen viability 32%-55%, transfection was performed at cool temperature using pretreated open the germination aperture (40%-55%). Magnetic nanoparticles (MNPs) coated with encoding either red fluorescent protein (RFP),...
Abstract Plants can grow in soils containing highly variable amounts of mineral nutrients, like Ca2+ and Mn2+, though the mechanisms adaptation are poorly understood. Here, we report first genetic study to determine vivo functions a pump plants. Homozygous mutants Arabidopsis harboring T-DNA disruption ECA1 showed 4-fold reduction endoplasmic reticulum-type calcium activity. Surprisingly, phenotype mutant plants was indistinguishable from wild type when grown on standard nutrient medium 1.5...
Abstract Ca2+ is required for protein processing, sorting, and secretion in eukaryotic cells, although the particular roles of transporters involved secretory system plants are obscure. One endomembrane-type Ca-ATPase from Arabidopsis (Arabidopsis thaliana), AtECA3, diverges AtECA1, AtECA2, AtECA4 sequence; yet, AtECA3 appears similar transport activity to endoplasmic reticulum (ER)-bound AtECA1. Expression a yeast (Saccharomyces cerevisiae) mutant defective its endogenous pumps conferred...
Abstract Purification of abscisic acid (ABA)-binding proteins is considered to constitute a major step toward isolating ABA receptors. We report here that an ABA-binding protein was for the first time, our knowledge, purified from epidermis broad bean (Vicia faba) leaves via affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, isoelectric focusing and focusing/sodium electrophoresis two-dimensional all identified single band with molecular mass 42 kD point...
Members of the R2R3-MYB transcription factor superfamily have been implicated in plant development, improved disease resistance, and defense responses to several types stresses. To study function TaMYB29 factor-a member superfamily-in response an avirulent race stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), we identified cloned gene from wheat cultivar (cv.) AvS+Yr10 following infection with Pst. The protein, comprising 261 amino acids, contains two highly conserved MYB...
The maize (Zea mays L.) glycosyltransferase family 1 comprises many uridine diphosphate (UGT) members. However, UGT activities and biochemical functions have seldom been revealed. In this study, the genes of two flavonoid di-O-glycosyltransferases ZmUGT84A1 ZmUGT84A2 were cloned from plant expressed in Escherichia coli. Phylogenetic analysis showed that enzymes homologous to AtUGT84A1 AtUGT84A3. recombinant a high conversion rate luteolin its glucosides, mainly 4′,7-di-O-glucoside minorly...
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a catastrophic disease that threatens global wheat yield. Yr10 race-specific all-stage resistance gene in wheat. However, the mechanism of poorly characterized. Therefore, to elucidate potential molecular mediated Yr10, transcriptomic sequencing was performed at 0, 18, and 48 h post-inoculation (hpi) compatible Avocet S (AvS) incompatible near-isogenic line (NIL) AvS + inoculated with Pst race CYR32. Respectively, 227, 208,...
Monolepta hieroglyphica, in view of its wide-ranging host and highly polyphagous characteristics, has become an important agricultural pest East Southeast Asian countries. To better understand biology develop control strategies, we present a high-quality chromosome-level genome assembly M. with contig N50 18.62 Mb scaffold 241.916 Mb. Using 156x Hi-C data, anchored total length 2,313 (91.9% the genome) to nine chromosomes. We predicted 46,561 genes using integrated approach. The completeness...
Abstract Drought tolerance is a comprehensive quantitative trait that being understood further at the molecular genetic level. Abscisic acid (ABA) main drought‐induced hormone regulates expression of many genes related to drought responses. 9‐cis‐epoxycarotenoid dioxygenase (NCED3) thought be key enzyme in ABA biosynthesis. In this paper, we measured content increase under stress, and sequenced compared sequence AtNCED 3 among 22 Arabidopsis thaliana accessions. The results showed fold...
Abstract Purpose To analyze the complete genome sequence of Brevibacterium frigoritolerans ZB201705, a strain was isolated from maize rhizosphere in drought- and salt-stressed soil, activity under simulated drought high salt conditions assessed. Methods We used combination PacBio RS Illumina sequencing platforms to obtain B. ZB201705. Results The consists 5,475,560 bp linear chromosome with no gaps, 4,391 protein-coding sequences, 39 ribosomal RNAs, 81 transfer RNAs. analysis revealed many...
Maize (Zea mays L.) silk contains high levels of flavonoids and is widely used to promote human health. Isoorientin, a natural C-glycoside flavone abundant in maize silk, has attracted considerable attention due its potential value. Although different classes flavonoid have been well characterized plants, the genes involved biosynthesis isoorientin are largely unknown. Here, we targeted metabolic profiling on silks an association panel consisting 294 inbred lines. We identified gene ZmCGT1...
Flavonoids are characteristic in maize and have diverse biological functions. C-Glycosylflavones neuroprotective against β-amyloid-induced tau hyperphosphorylation neurotoxicity SH-SY5Y cells, which is relevant to Alzheimer's disease prevention treatment. The content of the flavonoids eriodictyol, luteolin, isoorientin, maysin varied pollens, silks, tassels, seeds among five varieties. Eriodictyol was high (51-322 ng/g dw) while luteolin low (0.2-106 all four tissues. isoorientin...
Saline soil is a serious problem worldwide, and it necessary to improve the salt tolerance of plants so as avoid progressive deterioration saline soil. Here we report that over-expression AtNHX1 improves in transgenic tall fescue. The gene driven with CaMV35S promoter was constructed into plant expression vector pGreen0229, introduced embryonic calli hypocotyls fescue (Festuca arundinacea ) by particle bombardment. Regenerated plantlets were obtained screening herbicide (PPT, 2 mg/L),...
Basic helix-loop-helix (bHLH) transcription factors are widely distributed in eukaryotes, and plants, they regulate many biological processes, such as cell differentiation, development, metabolism, stress responses. Few studies have focused on the roles of bHLH regulating growth, responses maize (
Whether endovascular treatment (EVT) is safe and effective for vertebrobasilar artery occlusion (VBAO) yet incompletely understood. Two RCTs, the versus standard medical (BEST) trail Basilar Artery International Cooperation Study (BASICS), concentrating on this field were recently reported.We use real-world registry data of VBAO to compare outcome EVT inside outside inclusion exclusion criteria BEST BASICS study testify feasibility selection paradigms in these trials.Consecutive patients...