A5086658186- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Lung Cancer Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Renal and related cancers
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Cutaneous Melanoma Detection and Management
- Colorectal Cancer Treatments and Studies
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Lung Cancer Research Studies
- Single-cell and spatial transcriptomics
- Renal cell carcinoma treatment
- Antimicrobial Peptides and Activities
- Health, Environment, Cognitive Aging
- Glycosylation and Glycoproteins Research
- Liver Disease Diagnosis and Treatment
- Breast Cancer Treatment Studies
- Cancer Research and Treatments
- Fungal Infections and Studies
- Ferroptosis and cancer prognosis
- Immune cells in cancer
The University of Texas at Austin
2020-2025
Vanderbilt University
2006-2023
Vanderbilt-Ingram Cancer Center
2023
University School of Nashville
2023
Vanderbilt University Medical Center
2008-2013
University of Colorado Denver
2011
New York University
2010
University of Pittsburgh
2009-2010
University of Chicago
2009-2010
Johns Hopkins University
2010
Bacterial infection often results in the formation of tissue abscesses, which represent primary site interaction between invading bacteria and innate immune system. We identify host protein calprotectin as a neutrophil-dependent factor expressed inside Staphylococcus aureus abscesses. Neutrophil-derived inhibited S. growth through chelation nutrient Mn2+ Zn2+: an activity that reprogramming bacterial transcriptome. The abscesses mice lacking were enriched metal, staphylococcal proliferation...
Imaging MS (IMS) is an emerging technology that permits the direct analysis and determination of distribution molecules in tissue sections. Biological such as proteins, peptides, lipids, xenobiotics, metabolites can be analyzed a high-throughput manner with molecular specificity not readily achievable through other means. Tissues are intact thus spatial localization within preserved. Several studies presented focus on unique types information obtainable by IMS, Abeta isoform distributions...
MALDI imaging mass spectrometry (IMS) has become a valuable tool for the investigation of content and distribution molecular species in tissue specimens. Numerous methodological improvements have been made to optimize section preparation matrix deposition protocols, as well MS data acquisition processing. In particular proteomic analyses, washing sections before proven useful improve spectral qualities by increasing ion yields number signals observed. We systematically explore here effects...
To identify molecular markers of pathologic response to neoadjuvant paclitaxel/radiation treatment, protein and gene expression profiling were done on pretreatment biopsies.Patients with high-risk, operable breast cancer treated three cycles paclitaxel followed by concurrent paclitaxel/radiation. Tumor tissue from biopsies was obtained 19 the 38 patients enrolled in study. Protein serial sections that achieved a complete (pCR) compared those residual disease, non-pCR (NR).Proteomic...
Most platforms used for the molecular reconstruction of tumor–immune microenvironment (TIME) a solid tumor fail to explore spatial context three-dimensional (3D) space at single-cell resolution, and thus lack information about cell–cell or cell–extracellular matrix (ECM) interactions. To address this issue, pipeline which integrated multiplex spatially resolved multi-omics was developed identify crosstalk signaling networks among various cell types ECM in 3D TIME two FFPE (formalin-fixed...
Differentiating Spitz nevus (SN) from Spitzoid malignant melanoma (SMM) is one the most difficult problems in dermatopathology.To identify differences on proteomic level between SN and SMM.We performed Imaging Mass Spectrometry analysis formalin-fixed, paraffin-embedded tissue samples to SMM. The diagnosis of SMM was based histopathologic criteria, clinical features, follow-up data, which confirmed that none lesions diagnosed as recurred or metastasized. melanocytic component (tumor) tumor...
Nonalcoholic fatty liver disease (NAFLD) occurs frequently in a setting of obesity, dyslipidemia and insulin resistance, but the etiology disease, particularly events favoring progression to nonalcoholic steatohepatitis (NASH) as opposed simple steatosis (SS), are not fully understood. Based on known zonation patterns protein, glucose lipid metabolism, coupled with evidence that phosphatidylcholine may play role NASH pathogenesis, we hypothesized phospholipid exists specific abundance...
Intra-tumor heterogeneity (ITH) of human tumors is important for tumor progression, treatment response, and drug resistance. However, the spatial distribution ITH remains incompletely understood. Here, we present analysis in lung adenocarcinomas from 147 patients using multi-region mass spectrometry >5,000 regions, single-cell copy number sequencing ∼2,000 single cells, cyclic immunofluorescence >10 million cells. We identified two distinct patterns among tumors, termed clustered random...
Impairments in carbohydrate, lipid, and amino acid metabolism drive features of plaque instability. However, where these impairments occur within the atheroma remains largely unknown. Therefore, we sought to characterize spatial distribution metabolites stable unstable atherosclerosis both fibrous cap necrotic core.
Glutathione S-transferases (GSTs) are involved in many stress responses plants, for example, participating the detoxification of xenobiotics and limiting oxidative damage. Studies examining regulation this gene family diverse plant species have focused primarily on RNA expression. A proteomics method was developed to identify GSTs expressed Arabidopsis seedlings determine how abundance these proteins changed response copper, a promoter stress, benoxacor, herbicide safener. Eight were...
Abstract Early detection may help improve survival from lung cancer. In this study, our goal was to derive and validate a signature the proteomic analysis of bronchial lesions that could predict diagnosis Using previously published studies tissues, we selected nine matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) mass-to-charge ratio features build prediction model diagnostic The based on MALDI MS signal intensity score) tissue specimens 2005 cohort 51 patients....
Shotgun proteomics is a powerful analytic method to characterize complex protein mixtures in combination with multidimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS). We used this platform for proteomic characterization of apoptotic bodies an effort define the found primary cultures human intrahepatic biliary epithelial cells (HiBEC), renal proximal tubular cells, bronchial isolated from explanted cirrhosis (PBC), and control liver using total 24 individual samples....
Differentiating ulcerative colitis (UC) from Crohn's (CC) can be difficult and may lead to inaccurate diagnoses in up 30% of inflammatory bowel disease (IBD) patients. Much the diagnostic uncertainty arises overlap clinical histologic features. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) permits a histology-directed cellular protein analysis tissues. As pilot study, we evaluated ability MALDI-MS determine proteomic patterns for potential differences between CC UC...
Abstract Background: We hypothesized that a serum proteomic profile predictive of survival benefit in non–small cell lung cancer patients treated with epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) reflects tumor EGFR dependency regardless site origin or class therapeutic agent. Methods: Pretreatment plasma from 230 cetuximab, EGFR-TKIs, chemotherapy for recurrent/metastatic head and neck squamous carcinoma (HNSCC) colorectal (CRC) were analyzed by mass spectrometry....
Although Crohn's colitis (CC) and ulcerative (UC) share several clinical features, they have different causes, mechanisms of tissue damage, treatment options. Therefore, the accurate diagnosis is paramount importance in terms medical care. The distinction between CC/UC made on basis clinical, radiologic, endoscopic, pathologic interpretations but cannot be differentiated up to 15% inflammatory bowel disease patients. Correct management this "indeterminate colitis" depends accuracy future,...
Objective: To identify and image protein biomarker candidates in the synovial tissue of patients with rheumatoid arthritis (RA) osteoarthritis (OA).Methods: A novel matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) technique was applied to analysis tissue. Patients were classified according American College Rheumatology (ACR) criteria for RA. Frozen sections stained obtain morphological data. Serial desiccated, spotted matrix MALDI analysis. Ions generated...
No AccessJournal of UrologyInvestigative Urology1 Mar 2013Imaging the Clear Cell Renal Carcinoma Proteome Todd M. Morgan, Erin H. Seeley, Oluwole Fadare, Richard Caprioli, and Peter E. Clark MorganTodd Morgan Department Urologic Surgery, Vanderbilt University Medical Center, Nashville, Tennessee , SeeleyErin Seeley Biochemistry, Mass Spectrometry Research FadareOluwole Fadare Pathology, CaprioliRichard Caprioli ClarkPeter View All Author...