A5026593378- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Cancer therapeutics and mechanisms
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Telomeres, Telomerase, and Senescence
- Histone Deacetylase Inhibitors Research
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Brain Metastases and Treatment
- Protein Degradation and Inhibitors
- Effects of Radiation Exposure
- Protein Kinase Regulation and GTPase Signaling
- Drug Transport and Resistance Mechanisms
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- RNA Interference and Gene Delivery
- Polyomavirus and related diseases
- Melanoma and MAPK Pathways
- Chemotherapy-induced organ toxicity mitigation
University Medical Center of the Johannes Gutenberg University Mainz
2016-2025
Johannes Gutenberg University Mainz
2016-2025
Klinik und Poliklinik für Psychosomatische Medizin und Psychotherapie
2015-2023
University Hospital and Clinics
2022
University Medical Center
2012-2022
University of Cagliari
2017
Cancer Research UK Manchester Institute
2015
Heinrich Heine University Düsseldorf
2013
University of Applied Sciences Mainz
1997-2011
École Polytechnique Fédérale de Lausanne
2010
Small GTPases of the Rho family are involved in regulation a variety cellular processes, such as organization microfilamental network, cell-cell contact and malignant transformation. To address question whether proteins carcinogenesis man, we compared their expression tumors from colon, breast lung with that corresponding normal tissue originating same patient. As shown by Rho-specific 32P-ADP-ribosylation, well Western-blot analysis, amount RhoA protein was largely increased all 3 types...
Apoptosis, autophagy, necrosis and cellular senescence are key responses of cells that were exposed to genotoxicants. The types DNA damage triggering these their interrelationship largely unknown. Here we studied in glioma treated with the methylating agent temozolomide (TMZ), which is a first-line chemotherapeutic for this malignancy. We show upon TMZ treatment undergo apoptosis specific time-dependent manner. Necrosis was only marginally induced. All effects completely abrogated isogenic...
In the present study, we addressed question of a putative relevance Rho proteins in tumour progression by analysing their expression on protein and mRNA level breast tumours. We show that RhoA, RhoB, Rac1 Cdc42 is largely enhanced all samples analysed (n=15) as compared to normal tissues originating from same individual. The true for 32P-ADP-ribosylation which catalysed Clostridium botulinum exoenzyme C3. Also amount Rho-GDI ERK2 well overall 32P-GTP binding acvitity was tumour-specific...
Abstract Temozolomide (TMZ) is a methylating agent which prolongs survival when administered during and after radiotherapy in the first‐line treatment of glioblastoma also has significant activity recurrent disease. O 6 ‐methylguanine DNA methyltransferase (MGMT) repair enzyme attributed role cancer cell resistance to ‐alkylating agent‐based chemotherapy. Using panel 12 human glioma lines, we here defined sensitivity TMZ acute cytotoxicity clonogenic assays relation MGMT, mismatch p53 status...
“Replicative stress” is one of the main factors underlying neoplasia from its early stages. Genes involved in DNA synthesis may therefore represent an underexplored source potential prognostic markers for cancer. To this aim, we generated gene expression profiles two independent cohorts (France, n = 206; United Kingdom, 117) patients with previously untreated primary breast cancers. We report here that among 13 human nuclear polymerase genes, Polymerase θ ( POLQ ) only significantly...
Abstract Artesunate is a semisynthetic derivative from artemisinin, natural product the Chinese herb Artemisia annua L. It exerts antimalarial activity, and, additionally, artemisinin and its derivatives are active against cancer cells. The moiety an endoperoxide bridge. Its cleavage leads to formation of reactive oxygen species carbon-centered radicals. These highly molecules target several proteins in Plasmodia, which thought result killing microorganism. DNA damage induced by artemisinins...
Ras-homologous (Rho) GTPases play a pivotal role in the regulation of numerous cellular functions associated with malignant transformation and metastasis. Rho are localized at membranes become activated upon stimulation cell surface receptors. In their GTP-bound (= active) state, proteins bind to effector proteins, thereby triggering specific responses. Members family small key regulators actin reorganization, motility, cell-cell cell-extracellular matrix (ECM) adhesion as well cycle...