A5070163609- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- RNA modifications and cancer
- MicroRNA in disease regulation
- Probiotics and Fermented Foods
- Cancer-related molecular mechanisms research
- Protein Structure and Dynamics
- Circular RNAs in diseases
- Bacterial biofilms and quorum sensing
Centro de Investigaciones Biológicas Margarita Salas
2018-2025
Consejo Superior de Investigaciones Científicas
2018-2025
Universidad Complutense de Madrid
2017
Cytokinesis is a fundamental process for bacterial survival and proliferation, involving the formation of ring by filaments GTPase FtsZ, spatio-temporally regulated through coordinated action several factors. The mechanisms this regulation remain largely unsolved, but inhibition FtsZ polymerization nucleoid occlusion factor SlmA filament stabilization widely conserved cross-linking protein ZapA are known to play key roles. It was recently described that its target DNA sequences (SlmA-binding...
The division of an E. coli cell into two daughter cells with equal genomic information and similar size requires duplication segregation the chromosome subsequent scission envelope by a protein ring, Z-ring. MatP is DNA binding that contributes both to positioning Z-ring at midcell temporal control nucleoid segregation. Our integrated in vivo vitro analysis provides evidence can interact lipid membranes reproducing phospholipid mixture inner membrane, without concomitant recruitment short...
Dynamic biomolecular condensates formed by liquid–liquid phase separation can regulate the spatial and temporal organization of proteins, thus modulating their functional activity in cells. Previous studies showed that cell division protein FtsZ from Escherichia coli dynamic phase-separated with nucleoprotein complexes containing regulator SlmA under crowding conditions, potential implications for condensate-mediated spatiotemporal control division. In present study, we assessed formation...
Successful bacterial proliferation relies on the spatial and temporal precision of cytokinesis its regulation by systems that protect integrity nucleoid. In Escherichia coli , one these protectors is SlmA protein, which binds to specific DNA sites around nucleoid helps shield from inappropriate bisection cell division septum. Here, we discovered not only interacts with septum-associated proteins but also directly cytomimetic lipid membranes, adding a novel putative mechanism for regulating...
Abstract Macromolecular condensation resulting from biologically regulated liquid-liquid phase transitions is emerging as a mechanism to organize the intracellular space in eukaryotic systems, with broad implications cell physiology and pathology. Here we show that FtsZ, central element of division ring most bacteria, forms condensates when complex SlmA, protein preventing septal assembly nearby chromosome E. coli . The formation promoted by crowding enhanced sequence-specific binding SlmA...
ABSTRACT An increasing number of proteins involved in bacterial cell cycle events have been recently shown to undergo phase separation. The resulting biomolecular condensates play an important role protein function and may be development persister cells tolerant antibiotics. Here we report that the E. coli chromosomal Ter macrodomain organizer MatP, a division site selection implicated coordination chromosome segregation with division, forms cytomimetic systems. These are favored by crowding...
ABSTRACT Division ring formation at midcell is controlled by various mechanisms in Escherichia coli , one of them being the linkage between chromosomal Ter macrodomain and Z-ring mediated MatP, a DNA binding protein that organizes this contributes to prevention premature chromosome segregation. Here we show that, during cell division, just before splitting daughter cells, MatP seems localize close cytoplasmic membrane, suggesting might interact with lipids. To test hypothesis, investigated...
Biomolecular condensation through phase separation may be a novel mechanism to regulate bacterial processes, including cell division. Previous work revealed FtsZ, protein essential for cytokinesis in most bacteria, and the E. coli division site selection factor SlmA form FtsZ∙SlmA biomolecular condensates. The absence of condensates composed solely FtsZ under conditions used that study suggested this was restricted nucleoid occlusion or SlmA-containing bacteria. Here we report alone can...