A5045546872- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Metabolism and Genetic Disorders
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Pediatric Hepatobiliary Diseases and Treatments
- Pancreatic function and diabetes
- Liver physiology and pathology
- Metal complexes synthesis and properties
- Diet and metabolism studies
- Liver Diseases and Immunity
- Neonatal Health and Biochemistry
- Mitochondrial Function and Pathology
- Coenzyme Q10 studies and effects
- Magnetism in coordination complexes
- Crystallography and molecular interactions
- Organ Transplantation Techniques and Outcomes
- Peroxisome Proliferator-Activated Receptors
- Biochemical Acid Research Studies
- Mycotoxins in Agriculture and Food
- Adipose Tissue and Metabolism
- Liver Disease and Transplantation
- Metabolomics and Mass Spectrometry Studies
- Diabetes Treatment and Management
- Amino Acid Enzymes and Metabolism
Düsseldorf University Hospital
2016-2025
Heinrich Heine University Düsseldorf
2016-2025
University of Pittsburgh
2022
Children's Clinical University Hospital
2021
University Hospital Schleswig-Holstein
2017
University of Lübeck
2017
Helios Klinikum Krefeld
2017
Pediatrics and Genetics
2015
Boston Children's Hospital
2014
TU Dortmund University
2009-2012
Colorectal cancer (CRC) patients have been shown to possess an altered gut microbiome. Diet is a well-established modulator of the microbiome, and thus, dietary interventions might beneficial effect on CRC. An attenuating ketogenic diet (KD) CRC cell growth has previously observed, however role microbiome in driving this remains unknown. Here, we describe reduced colonic tumor burden upon KD consumption mouse model with humanized Importantly, demonstrate causal relationship through...
This paper reports a detailed theoretical study of the interaction between central low-spin d(8) nickel ion and two N,N-coordinating diiminobenzosemiquinonate(1-) ligands in square planar arrangement. Such complexes have recently attracted much attention due to their unusual bonding patterns, structures, optical, magnetic properties. Geometry optimizations using various levels density functional theory (DFT) result excellent agreement with experimentally determined structure particular...
Two series of square planar, diamagnetic, neutral complexes nickel(II), palladium(II), and platinum(II) containing two N,N-coordinated o-diiminobenzosemiquinonate(1-) π radical ligands have been synthesized characterized by UV−vis 1H NMR spectroscopy: [MII(2LISQ)2], M = Ni (1), Pd (2), Pt (3), [MII(3LISQ)2] (4), (5), (6). H2[2LPDI] represents 3,5-di-tert-butyl-o-phenylenediamine H2[3LPDI] is N-phenyl-o-phenylenediamine; (LISQ)1- the o-diiminobenzosemiquinonate anion, (LIBQ)0...
The bidentate ligands N-phenyl-o-phenylenediamine, H(2)((2)L(N)IP), or its analogue 2-(2-trifluoromethyl)anilino-4,6-di-tert-butylphenol, ((4)L(N)IP), react with [Co(II)(CH(3)CO(2))(2)]4H(2)O and triethylamine in acetonitrile the presence of air yielding square-planar, four-coordinate species [Co((2)L(N))(2)] (1) [Co((4)L(O))(2)] (4) an S=1/2 ground state. corresponding nickel complexes [Ni((4)L(O))(2)] (8) cobaltocene reduced form [Co(III)(Cp)(2)][Ni((4)L(O))(2)] (9) have also been...
<h3>Objective</h3> Cholestatic liver diseases in humans as well bile acid (BA)-feeding and common duct ligation (CBDL) rodents trigger hyperplasia of cholangiocytes within the portal fields. Furthermore, elevation BA levels enhances proliferation invasiveness cholangiocarcinoma (CCA) cells animal models, thus promoting tumour progression. TGR5 is a G-protein coupled receptor, which highly expressed postulated to mediate proliferative effects BA. <h3>Design</h3> BA-dependent cholangiocyte was...
Retinoid-storing hepatic stellate cells (HSCs) have recently been described as a liver-resident mesenchymal stem cell (MSC) population; however, it is not clear whether these contribute to liver regeneration or serve progenitor population with hepatobiliary characteristics. Here, we purified HSCs retinoid-dependent fluorescence-activated sorting from eGFP-expressing rats and transplanted GFP+ into wild-type (WT) that had undergone partial hepatectomy in the presence of 2-acetylaminofluorene...
Hepatic failure is commonly associated with anemia, which may result from gastrointestinal bleeding, vitamin deficiency, or liver‐damaging diseases, such as infection and alcohol intoxication. At least in theory, anemia during hepatic accelerated clearance of circulating erythrocytes. Here we show that bile duct ligation (BDL) mice leads to severe despite increased reticulocyte numbers. Bilirubin stimulated suicidal death human Mechanistically, bilirubin triggered rapid Ca 2+ influx,...
The ligand 2-mercapto-3,5-di-tert-butylaniline, H[LAP], an o-aminothiophenol, reacts with metal(II) salts of Ni and Pd in CH3CN or C2H5OH the presence NEt3 under strictly anaerobic conditions formation beige to yellow cis-[MII(LAP)2] (M = (1), (2)) where (LAP)1- represents o-aminothiophenolate(1-) form. crystal structure cis-[PdII(LAP)2][HN(C2H5)3][CH3CO2] has been determined by X-ray crystallography. In air same reaction produces dark blue solutions from which mixtures neutral complexes...
Abstract In the present study, we report application of LC‐MS based on two different systems to mycotoxin analysis. The mycotoxins were extracted with an ACN/water/acetic acid mixture and directly injected into a LC‐MS/MS system without any dilution procedure. First, sensitive reliable HPLC‐ESI‐MS/MS method using selected reaction monitoring triple quadrupole mass spectrometer (TSQ Quantum Ultra AM) has been developed for determining 32 in crude extracts wheat maize. This was operated both...
Significance Ammonia metabolism in the liver is critical to prevent serious clinical conditions, such as hepatic encephalopathy. It was hypothesized that Gln synthetase (GS) can metabolize ammonia with high affinity perivenous region of liver. However, vivo relevance this metabolic pathway remains unclear view other intra- and extrahepatic metabolizing pathways. Here, we show by creating a conditional GS KO mouse specific deletion results increased levels blood, induction oxidative stress...
Reversible ligand-centered one-electron oxidation of square-planar neutral complexes palladium(II) and platinum(II) [M(bpy)(L)] (bpy=bipyridine, L: see picture) leads to the paramagnetic [M(bpy)(L.)]+. Thus, existence S,S-coordinated o-dithiobenzosemiquinonate(1−) π radical ions has been proven unequivocally.
Abstract Mesenchymal stem cells (MSC) have the potential to differentiate into multiple cell lineages and their therapeutic has become obvious. In liver, MSC are represented by stellate which hepatocytes after stimulation with growth factors. Since bile acids can promote liver regeneration, influence on liver-resident bone marrow-derived was investigated. Physiological concentrations of such as tauroursodeoxycholic acid were able initiate hepatic differentiation via farnesoid X receptor...
We previously reported that inactivation of the transmembrane taurine transporter (TauT or solute carrier 6a6) causes early retinal degeneration in mice. Compatible with taurine's indispensability for cell volume homeostasis, protein stabilization, cytoprotection, antioxidation, and immuno- neuromodulation, mice develop multisystemic dysfunctions (hearing loss; liver fibrosis; behavioral, heart, skeletal muscle abnormalities) later on. Here, by genetic, biologic, vivo1H-magnetic resonance...
The DNA binding of cationic complexes the type [(η5-C5Me5)Ir(Aa)(dppz)](CF3SO3)n (dppz = dipyrido[3,2-a:2′,3′-c]phenazine; n 1, Aa AccysOH 7; 2, AcmetOMe 4, H2cysOMe 8; 3, H2metOMe 5) containing S-coordinated amino acids (HmetOH methionine, HcysOH cysteine) has been studied by UV-vis titration, 2D-NOESY and gel electrophoresis. observed steady decrease in absorbance at maxima between 350 400 nm on titration with CT bathochromic shifts these absorption are consistent stable intercalative for...
In non-alcoholic fatty liver disease (NAFLD) caused by ectopic lipid accumulation, lipotoxicity is a crucial molecular risk factor. Mechanisms to eliminate overflow can prevent the from functional complications. This may involve increased secretion of lipids or metabolic adaptation ß-oxidation in lipid-degrading organelles such as mitochondria and peroxisomes. addition dietary factors, plasma acid levels be due triglyceride synthesis, lipolysis, well de novo synthesis (DNL) liver. present...
Importance Perinatal stress and fetal growth restriction increase the risk of neonatal hypoglycemia. The underlying pathomechanism is poorly understood. In a sheep model, elevated catecholamine concentrations were found to suppress intrauterine insulin secretion, followed by hyperresponsive secretion once adrenergic stimulus subsided. Objective To determine whether neonates with factors for hypoglycemia have higher in umbilical cord blood (UCB) and/or amniotic fluid (AF) catecholamines are...
The coordination chemistry of 6-amino-2,4-di-tert-butylthiophenol (H[LSAP]) and 2-anilino-4,6-di-tert-butylphenol (H[LOAP]) with cobalt(II) ions was investigated in the presence absence dioxygen. It shown that both compounds were redox-non-innocent ligands, existing different protonation oxidation levels as: (i) o-aminophenolates(1−) [(LXAP)1−], (ii) o-imidophenolates(2−) [(LXIP)2−], (iii) one-electron oxidized forms o-iminobenzosemiquinonates(1−) [(LXISQ)1−], which are π-radicals (X = S, O...
The DNA binding of cationic complexes the type [(η6-arene)Ru(Aa)(dppz)] (CF3SO3)n (arene = C6H6, Me3C6H3, C6Me6; dppz dipyrido[3,2-a:2′,3′-c]phenazine; n 1, Aa AcH−1cysOH 4–6; 2, AcmetOH 7–9; 3, H2metOMe 10–12) containing S-coordinated amino acids (HcysOH L-cysteine, HmetOH L-methionine) has been studied by UV-vis titration and 2D-NOESY. Stable intercalative is indicated for these their steady decrease in absorbance at maxima between 350 390 nm on with CT bathochromic shifts absorption...
Abstract The metabolic profile of polar (methanol) and non‐polar (hexane) extracts Curcuma domestica , a widely used medicinal plant, was established using various different analytical techniques, including GC‐FID, GC‐MS, HR‐GC‐MS HPLC‐ESI‐MS/MS by means LTQ‐Orbitrap technology. major non‐volatile curcuminoids curcumin, demethoxycurcumin bisdemethoxycurcumin were identified when their chromatographic precursor ion masses compared with those authentic standard compounds. In this paper we...
Very long-chain acyl-CoA dehydrogenase (VLCAD)-deficiency is the most common fatty acid oxidation disorder presenting with heterogeneous phenotypes. Similar to many patients VLCADD, VLCAD-deficient mice (VLCAD−/−) remain asymptomatic over a long period of time. In order identify involved compensatory mechanisms, wild-type and VLCAD−/− were fed one year either normal diet or in which medium-chain triglycerides (MCT) replaced triglycerides, as approved intervention VLCADD. The expression...