A5007236317- Sirtuins and Resveratrol in Medicine
- Biochemical effects in animals
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Cancer-related molecular mechanisms research
- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Toxin Mechanisms and Immunotoxins
- Adipose Tissue and Metabolism
- Tea Polyphenols and Effects
- Antimicrobial Resistance in Staphylococcus
- RNA and protein synthesis mechanisms
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
Fort Lewis College
2019
It's a SIRT Intense attention has focused on the SIRT1 deacetylase as possible target for anti-aging drugs. But unexpected complications in assays of activity have made it unclear whether compounds thought to be sirtuin-activating (STACs) are really direct regulators enzyme. Further exploration these effects by Hubbard et al. (p. 1216 ; see Perspective Yuan and Marmorstein ) revealed that interaction with certain substrates allows activation STACs identified critical amino acids required...
SIRT1, the founding member of mammalian family seven NAD(+)-dependent sirtuins, is composed 747 amino acids forming a catalytic domain and extended N- C-terminal regions. We report design characterization an engineered human SIRT1 construct (mini-hSIRT1) containing minimal structural elements required for lysine deacetylation activation by small molecule sirtuin-activating compounds (STACs). Using this construct, we solved crystal structure mini-hSIRT1-STAC complex, which revealed...
Abstract Backbone amide hydrogen exchange rates can be used to describe the dynamic properties of a protein. Amide in native protein may vary from milliseconds (ms) several years. Ideally, all hydrogens analyte determined individually. To achieve this goal, monitoring wider time window is critical, addition high sequence coverage and resolution. Significant improvements have been made hydrogen/deuterium mass spectrometry methods past decade for better higher On other hand, little effort has...
Understanding antigen–antibody interactions at the sub‐molecular level is of particular interest for scientific, regulatory, and intellectual property reasons, especially with increasing demand monoclonal antibody therapeutic agents. Although various techniques are available determination an epitope, there no widely applicable, high‐resolution, reliable method available. Here, a combination approach using amide hydrogen/deuterium exchange coupled proteolysis mass spectrometry (HDX‐MS)...
TNFα (tumour necrosis factor α) is an early mediator in the systemic inflammatory response to infection and therefore a therapeutic target sepsis. AZD9773 ovine-derived, polyclonal anti-TNFα Fab fragment derived from pool of serum currently being developed as treatment for severe sepsis septic shock. In present study, we show that although has modest affinity binding assay, Ki cell-based assay approximately four orders magnitude lower. We using SEC (size exclusion chromatography) maximum...
Human Cytomegalovirus (HCMV) is a ubiquitous herpes virus that widespread and usually asymptomatic among healthy individuals. Congenital HCMV infections are the leading cause of virally induced birth defects. also causes severe disease in immunocompromised patients. Previous work our laboratory has shown CMV encodes large noncoding RNA called RNA5.0. Using an animal model, homologous murine model RNA7.2 been to help persist host salivary glands, but it unclear how this works at molecular...