A5074730124- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Insect symbiosis and bacterial influences
- Plant and Fungal Interactions Research
- Cocoa and Sweet Potato Agronomy
- Marine and coastal ecosystems
- Aquatic Ecosystems and Biodiversity
Mikocheni Agricultural Research Institute
2018-2024
University of Dar es Salaam
2022
In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with MinION and MinIT mobile sequencing devices Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The extracts using a cocktail of thermophilic proteinases cell wall-degrading enzymes, thermo-responsive extractor cartridges temperature control unit. This closed system delivers purified no cross-contamination. is newly released data...
Abstract Background Cassava leaf samples degrade quickly during storage and transportation from distant areas. Proper sampling efficient, low-cost methods are critical for obtaining sufficient quality DNA RNA plant virus epidemiology improving disease control understanding. This is useful when collected remote areas far a laboratory or in developing countries where money materials diagnostics scarce. Results The effect of sample duration on nucleic acid (N.A.) detection was investigated this...
<ns4:p>Crop losses due to viral diseases and pests are major constraints on food security income for millions of households in sub-Saharan Africa (SSA). Such can be reduced if plant correctly diagnosed identified early. Currently, accurate diagnosis definitive identification viruses their vectors SSA mostly relies standard PCR next generation sequencing technologies (NGS). However, it take up 6 months before results generated using these approaches available. The long time taken detect or...
Abstract In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with MinION and MinIT mobile sequencing devices Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The extracts using a cocktail of thermophilic proteinases cell wall degrading enzymes, thermo-responsive extractor cartridges temperature control unit. This single-step closed system delivers purified no cross contamination. is...
Abstract The United Nations has listed Zero Hunger as one of the 17 global sustainable development goals to end extreme poverty by 2030. Plant viruses are a major constraint crop production globally causing an estimated $30 billion in damage 1 leaving millions people food insecure 2 . In Africa, agriculture employs up 50% workforce, yet only contributes 15% GDP on average 3 , suggesting that there is low productivity and limited value addition. This can be addressed through continued...
Virus-related degeneration constrains production of quality sweet potato seed, especially under open field conditions. Once in the open, virus-indexed seed is prone to virus infection leading decline performance. Insect-proof net tunnels have been proven reduce researcher management. However, their effectiveness farmer-multiplier management not known. This study investigated ability Infection and were assessed for two cultivars, Kabode Polista, grown fields at sites with varying pressures....
Harmful algal blooms (HABs) pose a significant threat to aquatic ecosystems and human health due the production of toxins. The identification quantification these toxins are crucial for water quality management decisions. This study used DNA analysis (PCR techniques) identify toxin-producing strains liquid-chromatography-tandem mass spectrometry (LC-MS/MS) quantify microcystins in samples from Mindu Nyumba ya Mungu Dams Tanzania. results showed that HABs were detected both dams. BLAST...
<title>Abstract</title> <bold>Background:</bold> Cassava leaf samples can degrade quickly during storage and transportation from distant areas. Proper sampling the use of efficient cheap methods are critical to obtain sufficient quality DNA RNA for plant virus epidemiology improve understanding on disease control. This is practical when collected isolated zones a laboratory, or in developing countries that lack supply money materials diagnostics. <bold>Results: </bold>The effect sample...