A5054151882- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Animal Behavior and Reproduction
- Fish Ecology and Management Studies
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Neuroscience and Neuropharmacology Research
- Genomics and Rare Diseases
- Career Development and Diversity
- Metabolism and Genetic Disorders
- Signaling Pathways in Disease
- Reproductive Health and Technologies
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Coronary Artery Anomalies
- Congenital Heart Disease Studies
- Hair Growth and Disorders
- Angiogenesis and VEGF in Cancer
- Vasculitis and related conditions
- Nitric Oxide and Endothelin Effects
- Dermatology and Skin Diseases
- Pediatric Hepatobiliary Diseases and Treatments
- Genetic Neurodegenerative Diseases
- Renal Diseases and Glomerulopathies
- Genetic factors in colorectal cancer
Natera (United States)
2023
Bay Path University
2020-2022
Children's Hospital of Los Angeles
2010-2022
University of Minnesota
2005-2020
Pediatrics and Genetics
2019
Christopher Newport University
2019
Calgary Laboratory Services
2019
Surdna Foundation
2019
Institut thématique Génétique, génomique et bioinformatique
2015
University of Southern California
2010
A zebrafish heart can fully regenerate after amputation of up to 20% its ventricle. During this process, newly formed coronary blood vessels revascularize the regenerating tissue. The formation during regeneration likely recapitulates embryonic vessel development, which involves activation and proliferation epicardium, followed by an epithelial-to-mesenchymal transition. molecular cellular mechanisms underlying these processes are not well understood. We examined role PDGF signaling in...
Abstract Minnesota became the fourth state to begin newborn screening (NBS) for X‐linked adrenoleukodystrophy (X‐ALD) in 2017. As there is limited retrospective data available on NBS X‐ALD, we analyzed Minnesota's results from first year of screening. C26:0 lysophosphatidylcholine (C26:0‐LPC) 67,836 infants and confirmatory testing ( ABCD1 gene serum VLCFA analysis) screen positives were obtained. Fourteen (nine males, five females) screened positive X‐ALD all subsequently confirmed have...
Glutamatergic synapses switch from nonspiny to become dendritic spines during early neuronal development. Here, we report that the lack of sufficient Rac1, a small RhoGTPase, contributes absence spinogenesis in immature neurons. The overexpression green fluorescence protein-tagged wild-type Rac1 initiated formation cultured dissociated hippocampal neurons younger than 11 d vitro , indicating is likely one missing pieces responsible for also induced clustering AMPA receptors (AMPARs) and...
Clinical laboratories have adopted next generation sequencing (NGS) as a gold standard for the diagnosis of hereditary disorders because its analytic accuracy, high throughput, and potential cost-effectiveness. We describe implementation single broad-based NGS assay to meet genetic testing needs at University Minnesota. A hybrid capture library preparation was used each test ordered, data informatically blinded clinically-ordered genes, identified variants were reviewed classified by...
In the United States, persistence for women and ethnic minorities in science, technology, engineering, math (STEM) careers is strongly impacted by affective factors such as science identity, agency, sense of belonging. Policies aimed at increasing diversity national STEM student population workforce have recently focused on fostering inclusive learning environments that can positively impact experiences underrepresented (URMs) STEM, thus their retention. While research inclusion higher...
Background Platelet-derived growth factor receptor β (PDGFRβ) is a tyrosine kinase known to affect vascular development. The zebrafish an excellent model study specific regulators of development, yet the role PDGF signaling has not been determined in early embryos. Furthermore, mural cells, which PDGFRβ functions cell autonomously other systems, have identified embryos younger than 72 hours post fertilization. Methodology/Principal Findings In order investigate we cloned highly conserved...
In contrast to efforts focusing on improving inclusion in STEM classrooms from kindergarten through undergraduate (K–16), improve scientific meetings and conferences, important hubs of culture, are more recent. Markers that sometimes overlooked at these events can include the composition panels, how workshops run, affordability various other mechanisms maintain pre-existing hierarchies norms limit participation early-career researchers individuals minoritized cultural, linguistic, economic...
Endothelial cells emerge from the atrioventricular canal to form coronary blood vessels in juvenile zebrafish hearts. We find that pdgfrb is first expressed epicardium around and later becomes localized mainly mural cells. mutant fish show severe defects cell recruitment vessel development. Single-cell RNA sequencing analyses identified pdgfrb+ as epicardium-derived (EPDCs) Mural associated with arteries also express cxcl12b smooth muscle markers. Interestingly, these remain even absence of...
Abstract Alopecia areata (AA), a complex autoimmune hair loss condition, affects approximately 2.1% of the population. Individuals with AA have increased susceptibility to diseases such as atopy and disorders, but little is known about first‐degree relatives' risk develop associated conditions. Genetic counseling for multifactorial conditions, including disease complex, potentially valuable. Anecdotally we know patients ask medical providers recurrence family members well question whether...
The ATP-binding cassette transporter member A3 (ABCA3) is a lipid with critical function in pulmonary surfactant biogenesis. Biallelic loss-of-function mutations ABCA3 result severe deficiency leading to neonatal respiratory failure death the first year of life. Herein, we describe newborn distress at birth progressing requiring transplant. This patient was found have maternally inherited frameshift mutation and paternally synonymous variant predicted create cryptic splice site. Additional...
Zebrafish ( Danio rerio ) share a considerable amount of biological similarity with mammals, including identical or homologous gene expression pathways, neurotransmitters, hormones, and cellular receptors. also display complex social behaviors like shoaling schooling, making them an attractive model for investigating normal behavior as well exploring impaired function conditions such autism spectrum disorders. Newly-formed established shoals exhibit distinct patterns inter-member...
Abstract Zebrafish ( Danio rerio ) share a considerable amount of biological similarity with mammals, including identical or homologous gene expression pathways, neurotransmitters, hormones, and cellular receptors. also display complex social behaviors like shoaling schooling, making them an attractive model for investigating normal behavior as well exploring impaired function conditions such autism spectrum disorders. Newly-formed established shoals exhibit distinct patterns inter-member...
X-linked adrenoleukodystrophy (X-ALD) is the most common peroxisomal disorder, with an estimated incidence in USA of ~1:17,000 all births (male and female) 1:21,000 male births. X-ALD caused by mutations X chromosome gene ABCD1, which encodes membrane protein, ATP-binding cassette sub-family D member one, also known as or ALDP. ALDP transports very long chain fatty acids (VLCFA) from cytosol into peroxisome where VLCFA are metabolized beta-oxidation. Pathogenic ABCD1 cause absent abnormally...
Background: As broad-based genomics assays, such as whole exome and genome sequencing, are adopted clinical diagnostic tools, the identification reporting of incidental findings becomes a salient issue. The estimated chance identifying highly penetrant, pathogenic finding is between 1%-5% in individuals seeking sequencing. We elicited patient preferences regarding return developed methods for group …
ABSTRACT Endothelial cells emerge from the atrioventricular canal (AVC) to form nascent coronary blood vessels in juvenile zebrafish heart. We found that pdgfrβ is first expressed epicardium around AVC and later becomes localized mainly mural cells. mutant fish display severe defects cell recruitment vessel development. pdgfrβ+ are heterogeneous those associated with arteries also express cxcl12b . Mural positive for both transgenic reporters had elevated expression of smooth muscle genes....