A5013791617- Fish Ecology and Management Studies
- Genetic diversity and population structure
- Aquatic Invertebrate Ecology and Behavior
- Genetic and phenotypic traits in livestock
- Fish Biology and Ecology Studies
- Reproductive biology and impacts on aquatic species
- Environmental DNA in Biodiversity Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Marine Bivalve and Aquaculture Studies
- Parasite Biology and Host Interactions
- Chromosomal and Genetic Variations
- Identification and Quantification in Food
- Wildlife Ecology and Conservation
- Isotope Analysis in Ecology
- Glycosylation and Glycoproteins Research
- Marine Ecology and Invasive Species
- Lipid metabolism and disorders
- Sperm and Testicular Function
- Algal biology and biofuel production
- Cytomegalovirus and herpesvirus research
- Genomics and Phylogenetic Studies
- Marine Biology and Environmental Chemistry
- Marine Biology and Ecology Research
- Invertebrate Immune Response Mechanisms
- Evolution and Genetic Dynamics
Mie University
2015-2024
Nagoya University
2016
Kōchi University
1990-2009
Japanese Red Cross Kobe Hospital
2009
Japanese Red Cross Society, Japan
2009
Japan Fisheries Research and Education Agency
1998-2005
Dokkyo Medical University Saitama Medical Center
1994-2005
Dokkyo University
1994-2005
Yokohama National University
2002
Kirin (Japan)
1997
A novel hepatoma-derived growth factor was purified from the conditioned medium of human cell line, HuH-7, by assay [3H]thymidine incorporation into Swiss 3T3 cells. Molecular cloning a complementary DNA cDNA library HuH-7 cells done on basis N-terminal amino acid sequence. This protein acid- and heat-labile heparin-binding inactivated reducing condition. The is 2.4 kilobase pairs long, deduced sequence contained 240 acids without signal peptide-like hydrophobic Heparin column-eluted...
A lectin was isolated from the homogenate of tunicate Polyandrocarpa misakiensis by heat treatment, ammonium sulfate fractionation, gel filtration, and high-performance ion-exchange chromatography. Analytical filtration on Superose 12 sodium dodecyl sulfate-polyacrylamide electrophoresis showed that is a monomeric protein with molecular mass approximately 15 kDa. The bound to an immobilized D-galactose column in presence calcium ion threshold 500 microM eluted completely 5 mM EDTA. It did...
Abstract The bluegill sunfish, Lepomis macrochirus , is a notorious exotic species in many freshwater ecosystems, currently expanding its distribution worldwide. In 1960, small group of bluegills captured the Mississippi River at Guttenberg Iowa were imported to Japan as gift from mayor Chicago Japanese government. offspring these fish released into wild and also Korea. Over 40 years after this first introduction, L. now occupies all ecosystems both countries. We compared invading...
Bitterling (Teleostei: Acheilognathinae) are small cyprinid fishes with a discrete distribution in East Asia and Europe. We used complete mitochondrial cytochrome b sequence (1141 bp) from 49 species or subspecies three genera (Tanakia, Rhodeus, Acheilognathus), sampled across the major part of their distribution, to elucidate phylogeny biogeography, focusing particularly on origin dispersal. Based high support value, monophyletic Acheilognathinae separated into two clades, Acheilognathus...
Sperm of the Japanese bitterling Tanakia limbata that had been cryopreserved with 5 or 10% methanol plus 95 90% foetal bovine serum (FBS) showed higher percentage and longer duration motility than those FBS DMSO, glycerol, N,N‐dimethylacetamide N, N‐dimethylformamide. Foetal serum, used as extender, some cryoprotective effects when spermatozoa were cooled either without methanol. Spermatozoa, to −40° C then immersed in liquid nitrogen, greater post‐thaw −20, −60, −80° C. The motile increased...
Hermaphroditic freshwater clams in the genus Corbicula produce non-reductional spermatozoa. The DNA content of spermatozoa was almost identical with that somatic cells C. leana from Mie Prefecture, Japan. aff. fluminea Saga Prefecture and Taiwan also On other hand, dioecious sandai had half found cells. Analysis chromosome numbers suggests (3n = 54 18 meiotic cells) (2n 36 gills bivalents are triploids diploids, respectively. leana, fluminea, may lack either first or second meiosis,...
Two shell color types of the exotic bivalve Corbicula fluminea were collected in Kyoto city, Japan. DNA microfluorometry revealed that both diploids with non-reductional spermatozoa. Maternal chromosomes found to be extruded as two polar bodies at first meiosis, and second meiosis could not observed. Only male pronucleus was present egg cytoplasm became metaphase mitosis. The study indicates diploid C. Japan has same mode androgenetic reproduction triploid leana.
The Japanese rose bitterling, Rhodeus ocellatus kurumeus, is an endangered cyprinid species. main reason of drastic diminution R. o. kurumeus has been suggested to be due hybridization between this subspecies and introduced from China. Both RFLP analysis mitochondrial DNA RAPD-PCR genomic were performed elucidate the genetic introgression into kurumeus. two distinguished in D-loop region mtDNA by six restriction endonucleases. had eleven subspecies-specific RAPD markers two. Except some...
The mangrove killifish Kryptolebias marmoratus, and its close relative hermaphroditus, are the only vertebrate species known to reproduce by self-fertilization due functional ovotestis development. To improve our understanding of their genomes, we constructed a genetic map. First, single F1 fish was made artificial fertilization between K. marmoratus hermaphroditus strains. F2 progeny were then obtained fish. We used RAD-seq query genomic DNAs from two parental strains, individual 49...
In Mytilus mussels, paternal mitochondrial DNA (M type) from sperm is known to be transmitted offspring. This phenomenon called doubly uniparental inheritance (DUI). Under DUI, it has been reported that female mussels generally have only maternal mtDNA (F type). this study, we examined the mode of transmission in galloprovincialis using M and F type-specific primer sets. The ratio types were measured each sample by SNaPshot. type was detected adductor muscle gonad all females. unfertilized...
Abstract Climate oscillations during the Pleistocene had profound effects on evolutionary history of freshwater fishes now distributed across northern temperate regions. The extent continental glaciation western side North Pacific, including areas East Asia, was more limited as compared with regions America, Europe and high‐latitude Pacific. Therefore, climate might have influenced species in dissimilar ways depending species' distribution. We used mitochondrial DNA (mtDNA) microsatellite...
In Mytilus mussels, paternal mitochondrial DNA (mtDNA) from sperm is known to be transmitted offspring. This phenomenon called doubly uniparental inheritance (DUI). Under DUI, mtDNA (M type) inherited only by males. Female mussels receive maternal (F type). However, in our previous study, we showed female and unfertilized eggs have both F M types. We hypothesized that the two types were To test hypothesis, examined number of type haplotypes mature M. galloprovincialis . The larvae was...
We investigated the phylogeography of Asian brackish water clam, Corbicula japonica, to clarify its demographic history using partial mitochondrial COII gene sequences (990 bp) from 283 individuals collected around Japanese archipelago and adjacent areas. Phylogenetic analyses revealed presence two major groups within our samples: monophyletic Group I comprising Lineages A-E C. japonica paraphyletic II consisting sp. A-C were distributed in Japan Sakhalin Island, D, E, Korean Peninsula....
W e have cloned a human cDNA encoding for novel transmembrane protein from bladder carcinoma cell line (HT-1376) library.Sequence analysis of this clone revealed an open reading frame 2,691 bases 896 amino acids.Sequence comparison showed that has significant homology to desmocollins, intercellular adhesion molecules belonging the cadherin superfamily.This consists signal peptide 30 acids, precursor segment 105 and mature 761 acids.W expressed in COSl cells found predicted molecule remained...
The Japanese rosy bitterling, Rhodeus ocellatus kurumeus, has been affected not only by the invasion of another subspecies, R. o. ocellatus, from China, but also habitat fragmentation. In this study, effects fragmentation on fitness kurumeus were investigated. Owing to exclusion in Honshu and Shikoku disappeared entirely, except for small populations isolated man-made ponds Osaka Kagawa. Kyushu it still occupies open water systems, into which recently invaded. Meristic genetic data show that...